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CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma

Subpopulations of nasopharyngeal carcinoma (NPC) contain cells with differential tumourigenic properties. Our study evaluates the tumourigenic potential of CD24, CD44, EpCAM and combination of EpCAM/CD44 cells in NPC. CD44br and EpCAMbr cells enriched for higher S-phase cell content, fastergrowing t...

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Main Authors: Hoe, Susan Ling Ling, Tan, Lu Ping, Abdul Aziz, Norazlin, Liew, Kitson, Teow, Sin Yeang *, Abdul Razak, Fazlyn Reeny, Chin, Yoon Ming, Mohamed Shahrehan, Nurul Ashikin, Chu, Tai Lin, Mohd Kornain, Noor Kaslina, Peh, Suat Cheng *, Koay, Cheng Eng, Lo, Kwok-Wai, Ahmad, Munirah, Ng, Ching-Ching, Khoo, Alan Soo Beng
Format: Article
Language:English
Published: Nature Publishing Group 2017
Subjects:
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:http://eprints.sunway.edu.my/830/1/Ronald%20Teow%20CD24%20CD44.pdf
http://eprints.sunway.edu.my/830/
http://doi.org/10.1038/s41598-017-12045-8
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spelling my.sunway.eprints.8302019-05-13T08:42:32Z http://eprints.sunway.edu.my/830/ CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma Hoe, Susan Ling Ling Tan, Lu Ping Abdul Aziz, Norazlin Liew, Kitson Teow, Sin Yeang * Abdul Razak, Fazlyn Reeny Chin, Yoon Ming Mohamed Shahrehan, Nurul Ashikin Chu, Tai Lin Mohd Kornain, Noor Kaslina Peh, Suat Cheng * Koay, Cheng Eng Lo, Kwok-Wai Ahmad, Munirah Ng, Ching-Ching Khoo, Alan Soo Beng RC0254 Neoplasms. Tumors. Oncology (including Cancer) Subpopulations of nasopharyngeal carcinoma (NPC) contain cells with differential tumourigenic properties. Our study evaluates the tumourigenic potential of CD24, CD44, EpCAM and combination of EpCAM/CD44 cells in NPC. CD44br and EpCAMbr cells enriched for higher S-phase cell content, fastergrowing tumourigenic cells leading to tumours with larger volume and higher mitotic figures. Although CD44br and EpCAMbr cells significantly enriched for tumour-initiating cells (TICs), all cells could retain self-renewal property for at least four generations. Compared to CD44 marker alone, EpCAM/CD44dbr marker did not enhance for cells with faster-growing ability or higher TIC frequency. Cells expressing high CD44 or EpCAM had lower KLF4 and p21 in NPC subpopulations. KLF4-overexpressed EpCAMbr cells had slower growth while Kenpaullone inhibition of KLF4 transcription increased in vitro cell proliferation. Compared to non-NPC, NPC specimens had increased expression of EPCAM, of which tumours from advanced stage of NPC had higher expression. Together, our study provides evidence that EpCAM is a potentially important marker in NPC. Nature Publishing Group 2017-09-28 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/830/1/Ronald%20Teow%20CD24%20CD44.pdf Hoe, Susan Ling Ling and Tan, Lu Ping and Abdul Aziz, Norazlin and Liew, Kitson and Teow, Sin Yeang * and Abdul Razak, Fazlyn Reeny and Chin, Yoon Ming and Mohamed Shahrehan, Nurul Ashikin and Chu, Tai Lin and Mohd Kornain, Noor Kaslina and Peh, Suat Cheng * and Koay, Cheng Eng and Lo, Kwok-Wai and Ahmad, Munirah and Ng, Ching-Ching and Khoo, Alan Soo Beng (2017) CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma. Scientific Reports, 7 (1). p. 12372. ISSN 2045-2322 http://doi.org/10.1038/s41598-017-12045-8 doi:10.1038/s41598-017-12045-8
institution Sunway University
building Sunway Campus Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Sunway University
content_source Sunway Institutional Repository
url_provider http://eprints.sunway.edu.my/
language English
topic RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Hoe, Susan Ling Ling
Tan, Lu Ping
Abdul Aziz, Norazlin
Liew, Kitson
Teow, Sin Yeang *
Abdul Razak, Fazlyn Reeny
Chin, Yoon Ming
Mohamed Shahrehan, Nurul Ashikin
Chu, Tai Lin
Mohd Kornain, Noor Kaslina
Peh, Suat Cheng *
Koay, Cheng Eng
Lo, Kwok-Wai
Ahmad, Munirah
Ng, Ching-Ching
Khoo, Alan Soo Beng
CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma
description Subpopulations of nasopharyngeal carcinoma (NPC) contain cells with differential tumourigenic properties. Our study evaluates the tumourigenic potential of CD24, CD44, EpCAM and combination of EpCAM/CD44 cells in NPC. CD44br and EpCAMbr cells enriched for higher S-phase cell content, fastergrowing tumourigenic cells leading to tumours with larger volume and higher mitotic figures. Although CD44br and EpCAMbr cells significantly enriched for tumour-initiating cells (TICs), all cells could retain self-renewal property for at least four generations. Compared to CD44 marker alone, EpCAM/CD44dbr marker did not enhance for cells with faster-growing ability or higher TIC frequency. Cells expressing high CD44 or EpCAM had lower KLF4 and p21 in NPC subpopulations. KLF4-overexpressed EpCAMbr cells had slower growth while Kenpaullone inhibition of KLF4 transcription increased in vitro cell proliferation. Compared to non-NPC, NPC specimens had increased expression of EPCAM, of which tumours from advanced stage of NPC had higher expression. Together, our study provides evidence that EpCAM is a potentially important marker in NPC.
format Article
author Hoe, Susan Ling Ling
Tan, Lu Ping
Abdul Aziz, Norazlin
Liew, Kitson
Teow, Sin Yeang *
Abdul Razak, Fazlyn Reeny
Chin, Yoon Ming
Mohamed Shahrehan, Nurul Ashikin
Chu, Tai Lin
Mohd Kornain, Noor Kaslina
Peh, Suat Cheng *
Koay, Cheng Eng
Lo, Kwok-Wai
Ahmad, Munirah
Ng, Ching-Ching
Khoo, Alan Soo Beng
author_facet Hoe, Susan Ling Ling
Tan, Lu Ping
Abdul Aziz, Norazlin
Liew, Kitson
Teow, Sin Yeang *
Abdul Razak, Fazlyn Reeny
Chin, Yoon Ming
Mohamed Shahrehan, Nurul Ashikin
Chu, Tai Lin
Mohd Kornain, Noor Kaslina
Peh, Suat Cheng *
Koay, Cheng Eng
Lo, Kwok-Wai
Ahmad, Munirah
Ng, Ching-Ching
Khoo, Alan Soo Beng
author_sort Hoe, Susan Ling Ling
title CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma
title_short CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma
title_full CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma
title_fullStr CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma
title_full_unstemmed CD24, CD44 and EpCAM enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma
title_sort cd24, cd44 and epcam enrich for tumour-initiating cells in a newly established patient-derived xenograft of nasopharyngeal carcinoma
publisher Nature Publishing Group
publishDate 2017
url http://eprints.sunway.edu.my/830/1/Ronald%20Teow%20CD24%20CD44.pdf
http://eprints.sunway.edu.my/830/
http://doi.org/10.1038/s41598-017-12045-8
_version_ 1644324412104638464
score 13.211869

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