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Pteropine orthoreovirus of Bat Origin: An Emerging Pathogen Associated with Cases of Acute Respiratory Infections

Purpose Pteropine orthoreovirus (PRV) is a bat-borne virus classified under the Reoviridae family. It is a recently emerging virus in Southeast Asia and has been associated with sporadic respiratory infections in humans. Despite its epidemic potential, PRV is often neglected during diagnosis for ac...

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Main Authors:	Chan, Po Qhuan, Chook, Jack Bee *, Sam, I-Ching, Chan, Kok Gan, Voon, Kenny, Tee, Kok Keng
Format:	Article
Published:	Elsevier 2022
Subjects:	QL Zoology RA Public aspects of medicine RC Internal medicine
Online Access:	http://eprints.sunway.edu.my/2966/ https://doi.org/10.1016/j.ijid.2021.12.213
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spelling	my.sunway.eprints.29662024-08-04T02:35:05Z http://eprints.sunway.edu.my/2966/ Pteropine orthoreovirus of Bat Origin: An Emerging Pathogen Associated with Cases of Acute Respiratory Infections Chan, Po Qhuan Chook, Jack Bee * Sam, I-Ching Chan, Kok Gan Voon, Kenny Tee, Kok Keng QL Zoology RA Public aspects of medicine RC Internal medicine Purpose Pteropine orthoreovirus (PRV) is a bat-borne virus classified under the Reoviridae family. It is a recently emerging virus in Southeast Asia and has been associated with sporadic respiratory infections in humans. Despite its epidemic potential, PRV is often neglected during diagnosis for acute respiratory infection (ARI) and may be accountable for cases of unexplained ARI. The study aims to assess the molecular prevalence and analyze the genetic diversity of PRV in patients with ARI in an urban population. Methods & Materials A total of 3,935 nasopharyngeal swabs were collected from outpatients experiencing ARI symptoms in Kuala Lumpur, Malaysia from 2012 to 2014. Specimens were screened for all established respiratory viruses using a multiplex molecular assay. PCR-negative swabs were then screened for the sigma 1/A gene (major inner capsid) of PRV. Molecular epidemiological investigation and phylogenetic analysis were conducted using the global PRV strains isolated from humans and non-human hosts. Results Multiplex respiratory virus detection was negative for 49% of the patients. Among a subset of these patients (n=632), fourteen (2.2%) were tested positive for PRV. Patients (median age: 50.5, range 25-70 years old) were presented with mild to severe influenza-like illnesses. Phylogenetic analysis based on the sigma 1/A gene revealed thirteen (92.9%) patients infected with the Melaka virus (MelV)-like PRV. Limited inter-patient diversity of MelV-like PRV was estimated, suggesting a strong founding effect. Interestingly, a close ancestral relationship among MelV-like PRV strains isolated from the human and non-human (bats and monkey) hosts was observed. In addition, one patient was infected with the Pulau virus (PulV) previously found in fruit bats, indicating the first documented case of PulV-like PRV infection in human. Conclusion PRV of bat origin is a common causative agent of underdiagnosed ARI in the urban population. The study highlights the molecular prevalence of PRV among patients with ARI and the genetic diversity of PRV in the urban population of Kuala Lumpur, Malaysia. Elsevier 2022 Article PeerReviewed Chan, Po Qhuan and Chook, Jack Bee * and Sam, I-Ching and Chan, Kok Gan and Voon, Kenny and Tee, Kok Keng (2022) Pteropine orthoreovirus of Bat Origin: An Emerging Pathogen Associated with Cases of Acute Respiratory Infections. International Journal of Infectious Diseases, 116. ISSN 1878-3511 https://doi.org/10.1016/j.ijid.2021.12.213 10.1016/j.ijid.2021.12.213
institution	Sunway University
building	Sunway Campus Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Sunway University
content_source	Sunway Institutional Repository
url_provider	http://eprints.sunway.edu.my/
topic	QL Zoology RA Public aspects of medicine RC Internal medicine
spellingShingle	QL Zoology RA Public aspects of medicine RC Internal medicine Chan, Po Qhuan Chook, Jack Bee * Sam, I-Ching Chan, Kok Gan Voon, Kenny Tee, Kok Keng Pteropine orthoreovirus of Bat Origin: An Emerging Pathogen Associated with Cases of Acute Respiratory Infections
description	Purpose Pteropine orthoreovirus (PRV) is a bat-borne virus classified under the Reoviridae family. It is a recently emerging virus in Southeast Asia and has been associated with sporadic respiratory infections in humans. Despite its epidemic potential, PRV is often neglected during diagnosis for acute respiratory infection (ARI) and may be accountable for cases of unexplained ARI. The study aims to assess the molecular prevalence and analyze the genetic diversity of PRV in patients with ARI in an urban population. Methods & Materials A total of 3,935 nasopharyngeal swabs were collected from outpatients experiencing ARI symptoms in Kuala Lumpur, Malaysia from 2012 to 2014. Specimens were screened for all established respiratory viruses using a multiplex molecular assay. PCR-negative swabs were then screened for the sigma 1/A gene (major inner capsid) of PRV. Molecular epidemiological investigation and phylogenetic analysis were conducted using the global PRV strains isolated from humans and non-human hosts. Results Multiplex respiratory virus detection was negative for 49% of the patients. Among a subset of these patients (n=632), fourteen (2.2%) were tested positive for PRV. Patients (median age: 50.5, range 25-70 years old) were presented with mild to severe influenza-like illnesses. Phylogenetic analysis based on the sigma 1/A gene revealed thirteen (92.9%) patients infected with the Melaka virus (MelV)-like PRV. Limited inter-patient diversity of MelV-like PRV was estimated, suggesting a strong founding effect. Interestingly, a close ancestral relationship among MelV-like PRV strains isolated from the human and non-human (bats and monkey) hosts was observed. In addition, one patient was infected with the Pulau virus (PulV) previously found in fruit bats, indicating the first documented case of PulV-like PRV infection in human. Conclusion PRV of bat origin is a common causative agent of underdiagnosed ARI in the urban population. The study highlights the molecular prevalence of PRV among patients with ARI and the genetic diversity of PRV in the urban population of Kuala Lumpur, Malaysia.
format	Article
author	Chan, Po Qhuan Chook, Jack Bee * Sam, I-Ching Chan, Kok Gan Voon, Kenny Tee, Kok Keng
author_facet	Chan, Po Qhuan Chook, Jack Bee * Sam, I-Ching Chan, Kok Gan Voon, Kenny Tee, Kok Keng
author_sort	Chan, Po Qhuan
title	Pteropine orthoreovirus of Bat Origin: An Emerging Pathogen Associated with Cases of Acute Respiratory Infections
title_short	Pteropine orthoreovirus of Bat Origin: An Emerging Pathogen Associated with Cases of Acute Respiratory Infections
title_full	Pteropine orthoreovirus of Bat Origin: An Emerging Pathogen Associated with Cases of Acute Respiratory Infections
title_fullStr	Pteropine orthoreovirus of Bat Origin: An Emerging Pathogen Associated with Cases of Acute Respiratory Infections
title_full_unstemmed	Pteropine orthoreovirus of Bat Origin: An Emerging Pathogen Associated with Cases of Acute Respiratory Infections
title_sort	pteropine orthoreovirus of bat origin: an emerging pathogen associated with cases of acute respiratory infections
publisher	Elsevier
publishDate	2022
url	http://eprints.sunway.edu.my/2966/ https://doi.org/10.1016/j.ijid.2021.12.213
_version_	1806454420769079296
score	13.211869

Pteropine orthoreovirus of Bat Origin: An Emerging Pathogen Associated with Cases of Acute Respiratory Infections

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