Staff View: Gut microbiome profiling in systemic sclerosis: a metagenomic approach

Gut microbiome profiling in systemic sclerosis: a metagenomic approach

Objectives: The early gastrointestinal (GI) manifestation of systemic sclerosis (SSc) suggests a possible GI microbiota engagement in the pathophysiology and/or progression of SSc. Previous studies have revealed dysbiosis among Caucasian SSc patients. This study extends these findings to Asian SSc p...

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Main Authors:	Tan, Tze Chin, Chandrasekaran, Lakshmi, Leung, Ying Ying, Purbojati, Rikky, Pettersson, Sven *, Low, Andrea Hsiu Ling
Format:	Article
Published:	Clinical and Experimental Rheumatology 2023
Subjects:	QL Zoology RC Internal medicine RM Therapeutics. Pharmacology
Online Access:	http://eprints.sunway.edu.my/2832/ https://doi.org/10.55563/clinexprheumatol/jof7nx
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spelling	my.sunway.eprints.28322024-07-22T07:38:54Z http://eprints.sunway.edu.my/2832/ Gut microbiome profiling in systemic sclerosis: a metagenomic approach Tan, Tze Chin Chandrasekaran, Lakshmi Leung, Ying Ying Purbojati, Rikky Pettersson, Sven * Low, Andrea Hsiu Ling QL Zoology RC Internal medicine RM Therapeutics. Pharmacology Objectives: The early gastrointestinal (GI) manifestation of systemic sclerosis (SSc) suggests a possible GI microbiota engagement in the pathophysiology and/or progression of SSc. Previous studies have revealed dysbiosis among Caucasian SSc patients. This study extends these findings to Asian SSc patients. Methods: Adult SSc patients, stratified according to 1) on immunosuppressive (On-IS) drugs or 2) no immunosuppressive drugs (No-IS), and age-and-sex-matched healthy controls (HC) were recruited. Metagenomic sequencing of stool DNA was compared between SSc patients and HC, and between SSc (On-IS) and (No-IS) patients. Alpha and beta-diversity, taxonomic and functional profiling were evaluated. Results: Twenty-three female SSc patients (12 On-IS; 11 No-IS; 5 diffuse and 18 limited SSc subtype) and 19 female HC, with median age of 54 years and 56 years, respectively, were recruited. Median SSc disease duration was 3.3 years. Alpha diversity was significantly higher in SSc versus HC (p=0.014) and in SSc (No-IS) versus HC (p=0.006). There was no significant difference in beta diversity between SSc and HC (p=0.307). At the phyla level, there were significantly increased abundance of Firmicutes and Actinobacteria in SSc versus HC, and reduced abundance of Bacteroidetes (all p<0.001). At the species level, there were significantly increased abundance of several Lactobacillus, Bifidobacterium, and Coprococcus species in SSc, and increased abundance of Odoribacter, Bacteroides and Prevotella species in HC. KEGG pathway analysis demonstrated distinct differences between SSc versus HC, and between SSc (No-IS) and SSc (On-IS). Conclusions: Using metagenomic sequencing, our study further underlines distinct alterations in microbiota profiling among Asian SSc patients. Clinical and Experimental Rheumatology 2023 Article PeerReviewed Tan, Tze Chin and Chandrasekaran, Lakshmi and Leung, Ying Ying and Purbojati, Rikky and Pettersson, Sven * and Low, Andrea Hsiu Ling (2023) Gut microbiome profiling in systemic sclerosis: a metagenomic approach. Clinical and Experimental Rheumatology, 41 (8). ISSN 0392-856x https://doi.org/10.55563/clinexprheumatol/jof7nx 10.55563/clinexprheumatol/jof7nx
institution	Sunway University
building	Sunway Campus Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Sunway University
content_source	Sunway Institutional Repository
url_provider	http://eprints.sunway.edu.my/
topic	QL Zoology RC Internal medicine RM Therapeutics. Pharmacology
spellingShingle	QL Zoology RC Internal medicine RM Therapeutics. Pharmacology Tan, Tze Chin Chandrasekaran, Lakshmi Leung, Ying Ying Purbojati, Rikky Pettersson, Sven * Low, Andrea Hsiu Ling Gut microbiome profiling in systemic sclerosis: a metagenomic approach
description	Objectives: The early gastrointestinal (GI) manifestation of systemic sclerosis (SSc) suggests a possible GI microbiota engagement in the pathophysiology and/or progression of SSc. Previous studies have revealed dysbiosis among Caucasian SSc patients. This study extends these findings to Asian SSc patients. Methods: Adult SSc patients, stratified according to 1) on immunosuppressive (On-IS) drugs or 2) no immunosuppressive drugs (No-IS), and age-and-sex-matched healthy controls (HC) were recruited. Metagenomic sequencing of stool DNA was compared between SSc patients and HC, and between SSc (On-IS) and (No-IS) patients. Alpha and beta-diversity, taxonomic and functional profiling were evaluated. Results: Twenty-three female SSc patients (12 On-IS; 11 No-IS; 5 diffuse and 18 limited SSc subtype) and 19 female HC, with median age of 54 years and 56 years, respectively, were recruited. Median SSc disease duration was 3.3 years. Alpha diversity was significantly higher in SSc versus HC (p=0.014) and in SSc (No-IS) versus HC (p=0.006). There was no significant difference in beta diversity between SSc and HC (p=0.307). At the phyla level, there were significantly increased abundance of Firmicutes and Actinobacteria in SSc versus HC, and reduced abundance of Bacteroidetes (all p<0.001). At the species level, there were significantly increased abundance of several Lactobacillus, Bifidobacterium, and Coprococcus species in SSc, and increased abundance of Odoribacter, Bacteroides and Prevotella species in HC. KEGG pathway analysis demonstrated distinct differences between SSc versus HC, and between SSc (No-IS) and SSc (On-IS). Conclusions: Using metagenomic sequencing, our study further underlines distinct alterations in microbiota profiling among Asian SSc patients.
format	Article
author	Tan, Tze Chin Chandrasekaran, Lakshmi Leung, Ying Ying Purbojati, Rikky Pettersson, Sven * Low, Andrea Hsiu Ling
author_facet	Tan, Tze Chin Chandrasekaran, Lakshmi Leung, Ying Ying Purbojati, Rikky Pettersson, Sven * Low, Andrea Hsiu Ling
author_sort	Tan, Tze Chin
title	Gut microbiome profiling in systemic sclerosis: a metagenomic approach
title_short	Gut microbiome profiling in systemic sclerosis: a metagenomic approach
title_full	Gut microbiome profiling in systemic sclerosis: a metagenomic approach
title_fullStr	Gut microbiome profiling in systemic sclerosis: a metagenomic approach
title_full_unstemmed	Gut microbiome profiling in systemic sclerosis: a metagenomic approach
title_sort	gut microbiome profiling in systemic sclerosis: a metagenomic approach
publisher	Clinical and Experimental Rheumatology
publishDate	2023
url	http://eprints.sunway.edu.my/2832/ https://doi.org/10.55563/clinexprheumatol/jof7nx
_version_	1805893820013871104
score	13.211869

Gut microbiome profiling in systemic sclerosis: a metagenomic approach

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