Staff View: Pathogenic fungi of Rhodotorula Dairenensis is linked with colorectal cancer patients in Malaysia

Pathogenic fungi of Rhodotorula Dairenensis is linked with colorectal cancer patients in Malaysia

Background Colorectal cancer (CRC) is the second most lethal disease with about 1.9 million new cases and 0.9 million fatalities worldwide in 2020. It is expected that the CRC prevalence to rise steadily each year. Several studies have linked the gut microbiome to CRC, particularly emphasizing the p...

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Main Authors:	Aisyah, Yunus, Norfilza, Mohd Mokhtar, Raja Affendi, Raja Ali *, Siti Maryam, Ahmad Kendong, Nielsen, Dennis Sandris, Hajar, Fauzan Ahmad
Format:	Article
Published:	BMJ Publishing Group 2023
Subjects:	QR Microbiology RC Internal medicine
Online Access:	http://eprints.sunway.edu.my/2799/ https://doi.org/10.1136/gutjnl-2023-IDDF.116
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spelling	my.sunway.eprints.27992024-07-12T03:19:58Z http://eprints.sunway.edu.my/2799/ Pathogenic fungi of Rhodotorula Dairenensis is linked with colorectal cancer patients in Malaysia Aisyah, Yunus Norfilza, Mohd Mokhtar Raja Affendi, Raja Ali * Siti Maryam, Ahmad Kendong Nielsen, Dennis Sandris Hajar, Fauzan Ahmad QR Microbiology RC Internal medicine Background Colorectal cancer (CRC) is the second most lethal disease with about 1.9 million new cases and 0.9 million fatalities worldwide in 2020. It is expected that the CRC prevalence to rise steadily each year. Several studies have linked the gut microbiome to CRC, particularly emphasizing the prokaryotic communities’ functions. However, it is unclear how other gut microbiota components, such as fungal communities, could be related to the pathogenesis of CRC. Hence, we aimed to explore the role of opportunistic fungal pathogens and the host’s phenotypes among CRC patients. Methods Biopsy samples were obtained during colonoscopy sessions from 64 individuals. Of which, 32 are colorectal cancer patients comprising the early-onset CRC, and late-onset CRC groups, 22 are diagnosed with polyps during colonoscopy and the remaining are rectal swabs from normal individuals without any previous disease history. Informed consents were obtained from all patients before collecting their biopsy samples. The g DNA were extracted using Ultra Deep Microbiome Prep Kit. Prior to sequencing, the amplicons of microbial genome libraries were by targeting the ITS1 regions. Finally, the microbial genomic data were analysed using state-of-art bioinformatic tools. Results A total of 6,477,706 read counts were generated, representing 1,364 amplicon sequence variants of fungi. At phyla, Ascomycota, Basidiomycota, Mortierellomycota, and Chytridiomycota were mainly found in both early and late-onset CRC patients. Moreover, the alpha-diversity showed significant differences between early and late-onset CRC patients, polyps, and normal individuals; Chao1 diversity (p-value = 0.0017509). Based on Linear discriminant analysis Effect Size analysis, the species Rhodotorula dairenensis was found to have a positive correlation for both early and late-onset colorectal cancer patients. Conclusions Our findings imply the correlation between the presence of opportunistic fungal species Rhodotorula among CRC patients in Malaysia. Previous studies reported that cancer patients are at higher risk for Rhodotorula infection. However, further study is needed in order to elucidate the role of the opportunistic pathogen during disease progression. BMJ Publishing Group 2023 Article PeerReviewed Aisyah, Yunus and Norfilza, Mohd Mokhtar and Raja Affendi, Raja Ali * and Siti Maryam, Ahmad Kendong and Nielsen, Dennis Sandris and Hajar, Fauzan Ahmad (2023) Pathogenic fungi of Rhodotorula Dairenensis is linked with colorectal cancer patients in Malaysia. Gut, 72. ISSN 1468-3288 https://doi.org/10.1136/gutjnl-2023-IDDF.116 10.1136/gutjnl-2023-IDDF.116
institution	Sunway University
building	Sunway Campus Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Sunway University
content_source	Sunway Institutional Repository
url_provider	http://eprints.sunway.edu.my/
topic	QR Microbiology RC Internal medicine
spellingShingle	QR Microbiology RC Internal medicine Aisyah, Yunus Norfilza, Mohd Mokhtar Raja Affendi, Raja Ali * Siti Maryam, Ahmad Kendong Nielsen, Dennis Sandris Hajar, Fauzan Ahmad Pathogenic fungi of Rhodotorula Dairenensis is linked with colorectal cancer patients in Malaysia
description	Background Colorectal cancer (CRC) is the second most lethal disease with about 1.9 million new cases and 0.9 million fatalities worldwide in 2020. It is expected that the CRC prevalence to rise steadily each year. Several studies have linked the gut microbiome to CRC, particularly emphasizing the prokaryotic communities’ functions. However, it is unclear how other gut microbiota components, such as fungal communities, could be related to the pathogenesis of CRC. Hence, we aimed to explore the role of opportunistic fungal pathogens and the host’s phenotypes among CRC patients. Methods Biopsy samples were obtained during colonoscopy sessions from 64 individuals. Of which, 32 are colorectal cancer patients comprising the early-onset CRC, and late-onset CRC groups, 22 are diagnosed with polyps during colonoscopy and the remaining are rectal swabs from normal individuals without any previous disease history. Informed consents were obtained from all patients before collecting their biopsy samples. The g DNA were extracted using Ultra Deep Microbiome Prep Kit. Prior to sequencing, the amplicons of microbial genome libraries were by targeting the ITS1 regions. Finally, the microbial genomic data were analysed using state-of-art bioinformatic tools. Results A total of 6,477,706 read counts were generated, representing 1,364 amplicon sequence variants of fungi. At phyla, Ascomycota, Basidiomycota, Mortierellomycota, and Chytridiomycota were mainly found in both early and late-onset CRC patients. Moreover, the alpha-diversity showed significant differences between early and late-onset CRC patients, polyps, and normal individuals; Chao1 diversity (p-value = 0.0017509). Based on Linear discriminant analysis Effect Size analysis, the species Rhodotorula dairenensis was found to have a positive correlation for both early and late-onset colorectal cancer patients. Conclusions Our findings imply the correlation between the presence of opportunistic fungal species Rhodotorula among CRC patients in Malaysia. Previous studies reported that cancer patients are at higher risk for Rhodotorula infection. However, further study is needed in order to elucidate the role of the opportunistic pathogen during disease progression.
format	Article
author	Aisyah, Yunus Norfilza, Mohd Mokhtar Raja Affendi, Raja Ali * Siti Maryam, Ahmad Kendong Nielsen, Dennis Sandris Hajar, Fauzan Ahmad
author_facet	Aisyah, Yunus Norfilza, Mohd Mokhtar Raja Affendi, Raja Ali * Siti Maryam, Ahmad Kendong Nielsen, Dennis Sandris Hajar, Fauzan Ahmad
author_sort	Aisyah, Yunus
title	Pathogenic fungi of Rhodotorula Dairenensis is linked with colorectal cancer patients in Malaysia
title_short	Pathogenic fungi of Rhodotorula Dairenensis is linked with colorectal cancer patients in Malaysia
title_full	Pathogenic fungi of Rhodotorula Dairenensis is linked with colorectal cancer patients in Malaysia
title_fullStr	Pathogenic fungi of Rhodotorula Dairenensis is linked with colorectal cancer patients in Malaysia
title_full_unstemmed	Pathogenic fungi of Rhodotorula Dairenensis is linked with colorectal cancer patients in Malaysia
title_sort	pathogenic fungi of rhodotorula dairenensis is linked with colorectal cancer patients in malaysia
publisher	BMJ Publishing Group
publishDate	2023
url	http://eprints.sunway.edu.my/2799/ https://doi.org/10.1136/gutjnl-2023-IDDF.116
_version_	1805893814127165440
score	13.211869

Pathogenic fungi of Rhodotorula Dairenensis is linked with colorectal cancer patients in Malaysia

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