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Applications of Polyaniline-Based Molybdenum Disulfide Nanoparticles against Brain-Eating Amoebae

Primary amoebic meningoencephalitis and granulomatous amoebic encephalitis are distressing infections of the central nervous system caused by brain-eating amoebae, namely, Naegleria fowleri and Acanthamoeba spp., respectively, and present mortality rates of over 90%. No single drug has been approved...

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Main Authors: Sumayah, Abdelnasir, Mungroo, Mohammad Ridwane, Chew, Jactty *, Siddiqui, Ruqaiyyah, Khan, Naveed Ahmed, Ahmad, Irfan, Shahabuddin, Syed, Ayaz, Anwar *
Format: Article
Language:English
Published: American Chemical Society 2023
Subjects:
QL Zoology
QP Physiology
RM Therapeutics. Pharmacology
Online Access:http://eprints.sunway.edu.my/2733/1/Chew%20Jactty_Applications%20of%20Polyaniline-Based%20Molybdenum%20Disulfide%20Nanoparticles.pdf
http://eprints.sunway.edu.my/2733/
https://doi.org/10.1021/acsomega.2c06050
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spelling my.sunway.eprints.27332024-07-04T06:31:12Z http://eprints.sunway.edu.my/2733/ Applications of Polyaniline-Based Molybdenum Disulfide Nanoparticles against Brain-Eating Amoebae Sumayah, Abdelnasir Mungroo, Mohammad Ridwane Chew, Jactty * Siddiqui, Ruqaiyyah Khan, Naveed Ahmed Ahmad, Irfan Shahabuddin, Syed Ayaz, Anwar * QL Zoology QP Physiology RM Therapeutics. Pharmacology Primary amoebic meningoencephalitis and granulomatous amoebic encephalitis are distressing infections of the central nervous system caused by brain-eating amoebae, namely, Naegleria fowleri and Acanthamoeba spp., respectively, and present mortality rates of over 90%. No single drug has been approved for use against these infections, and current therapy is met with an array of obstacles including high toxicity and limited specificity. Thus, the development of alternative effective chemotherapeutic agents for the management of infections due to brain-eating amoebae is a crucial requirement to avert future mortalities. In this paper, we synthesized a conducting polymer-based nanocomposite entailing polyaniline (PANI) and molybdenum disulfide (MoS2) and explored its anti-trophozoite and anti-cyst potentials against Acanthamoeba castellanii and Naegleria fowleri. The intracellular generation of reactive oxygen species (ROS) and ultrastructural appearances of amoeba were also evaluated with treatment. Throughout, treatment with the 1:2 and 1:5 ratios of PANI/MoS2 at 100 μg/mL demonstrated significant anti-amoebic effects toward A. castellanii as well as N. fowleri, appraised to be ROS mediated and effectuate physical alterations to amoeba morphology. Further, cytocompatibility toward human keratinocyte skin cells (HaCaT) and primary human corneal epithelial cells (pHCEC) was noted. For the first time, polymer-based nanocomposites such as PANI/MoS2 are reported in this study as appealing options in the drug discovery for brain-eating amoebae infections. American Chemical Society 2023 Article PeerReviewed text en cc_by_nc_nd_4 http://eprints.sunway.edu.my/2733/1/Chew%20Jactty_Applications%20of%20Polyaniline-Based%20Molybdenum%20Disulfide%20Nanoparticles.pdf Sumayah, Abdelnasir and Mungroo, Mohammad Ridwane and Chew, Jactty * and Siddiqui, Ruqaiyyah and Khan, Naveed Ahmed and Ahmad, Irfan and Shahabuddin, Syed and Ayaz, Anwar * (2023) Applications of Polyaniline-Based Molybdenum Disulfide Nanoparticles against Brain-Eating Amoebae. ACS Omega, 8. pp. 8237-8247. ISSN 2470-1343 https://doi.org/10.1021/acsomega.2c06050 10.1021/acsomega.2c06050
institution Sunway University
building Sunway Campus Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Sunway University
content_source Sunway Institutional Repository
url_provider http://eprints.sunway.edu.my/
language English
topic QL Zoology
QP Physiology
RM Therapeutics. Pharmacology
spellingShingle QL Zoology
QP Physiology
RM Therapeutics. Pharmacology
Sumayah, Abdelnasir
Mungroo, Mohammad Ridwane
Chew, Jactty *
Siddiqui, Ruqaiyyah
Khan, Naveed Ahmed
Ahmad, Irfan
Shahabuddin, Syed
Ayaz, Anwar *
Applications of Polyaniline-Based Molybdenum Disulfide Nanoparticles against Brain-Eating Amoebae
description Primary amoebic meningoencephalitis and granulomatous amoebic encephalitis are distressing infections of the central nervous system caused by brain-eating amoebae, namely, Naegleria fowleri and Acanthamoeba spp., respectively, and present mortality rates of over 90%. No single drug has been approved for use against these infections, and current therapy is met with an array of obstacles including high toxicity and limited specificity. Thus, the development of alternative effective chemotherapeutic agents for the management of infections due to brain-eating amoebae is a crucial requirement to avert future mortalities. In this paper, we synthesized a conducting polymer-based nanocomposite entailing polyaniline (PANI) and molybdenum disulfide (MoS2) and explored its anti-trophozoite and anti-cyst potentials against Acanthamoeba castellanii and Naegleria fowleri. The intracellular generation of reactive oxygen species (ROS) and ultrastructural appearances of amoeba were also evaluated with treatment. Throughout, treatment with the 1:2 and 1:5 ratios of PANI/MoS2 at 100 μg/mL demonstrated significant anti-amoebic effects toward A. castellanii as well as N. fowleri, appraised to be ROS mediated and effectuate physical alterations to amoeba morphology. Further, cytocompatibility toward human keratinocyte skin cells (HaCaT) and primary human corneal epithelial cells (pHCEC) was noted. For the first time, polymer-based nanocomposites such as PANI/MoS2 are reported in this study as appealing options in the drug discovery for brain-eating amoebae infections.
format Article
author Sumayah, Abdelnasir
Mungroo, Mohammad Ridwane
Chew, Jactty *
Siddiqui, Ruqaiyyah
Khan, Naveed Ahmed
Ahmad, Irfan
Shahabuddin, Syed
Ayaz, Anwar *
author_facet Sumayah, Abdelnasir
Mungroo, Mohammad Ridwane
Chew, Jactty *
Siddiqui, Ruqaiyyah
Khan, Naveed Ahmed
Ahmad, Irfan
Shahabuddin, Syed
Ayaz, Anwar *
author_sort Sumayah, Abdelnasir
title Applications of Polyaniline-Based Molybdenum Disulfide Nanoparticles against Brain-Eating Amoebae
title_short Applications of Polyaniline-Based Molybdenum Disulfide Nanoparticles against Brain-Eating Amoebae
title_full Applications of Polyaniline-Based Molybdenum Disulfide Nanoparticles against Brain-Eating Amoebae
title_fullStr Applications of Polyaniline-Based Molybdenum Disulfide Nanoparticles against Brain-Eating Amoebae
title_full_unstemmed Applications of Polyaniline-Based Molybdenum Disulfide Nanoparticles against Brain-Eating Amoebae
title_sort applications of polyaniline-based molybdenum disulfide nanoparticles against brain-eating amoebae
publisher American Chemical Society
publishDate 2023
url http://eprints.sunway.edu.my/2733/1/Chew%20Jactty_Applications%20of%20Polyaniline-Based%20Molybdenum%20Disulfide%20Nanoparticles.pdf
http://eprints.sunway.edu.my/2733/
https://doi.org/10.1021/acsomega.2c06050
_version_ 1804069664563658752
score 13.211869

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