Staff View: Novel cationic cryptides in Penaeus vannamei demonstrate antimicrobial and anti-cancer activities

Novel cationic cryptides in Penaeus vannamei demonstrate antimicrobial and anti-cancer activities

Cryptides are a subfamily of bioactive peptides that exist in all living organisms. They are latently encrypted in their parent sequences and exhibit a wide range of biological activities when decrypted via in vivo or in vitro proteases. Cationic cryptides tend to be drawn to the negatively charged...

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Main Authors:	Amr Adel, Ahmed Abd El-Aal , Fairen, Angelin Jayakumar , Lahiri, Chandrajit , Tan, Kuan Onn , Kavita, Reginald *
Format:	Article
Language:	English
Published:	Nature Publishing Group 2023
Subjects:	QL Zoology RC Internal medicine RM Therapeutics. Pharmacology
Online Access:	http://eprints.sunway.edu.my/2684/1/Kavita%20Reginald_Novel%20cationic%20cryptides%20in%20Penaeus%20vannamei%20demonstrate.pdf http://eprints.sunway.edu.my/2684/ https://doi.org/10.1038/s41598-023-41581-9
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spelling	my.sunway.eprints.26842024-06-24T05:59:58Z http://eprints.sunway.edu.my/2684/ Novel cationic cryptides in Penaeus vannamei demonstrate antimicrobial and anti-cancer activities Amr Adel, Ahmed Abd El-Aal * Fairen, Angelin Jayakumar * Lahiri, Chandrajit * Tan, Kuan Onn * Kavita, Reginald * QL Zoology RC Internal medicine RM Therapeutics. Pharmacology Cryptides are a subfamily of bioactive peptides that exist in all living organisms. They are latently encrypted in their parent sequences and exhibit a wide range of biological activities when decrypted via in vivo or in vitro proteases. Cationic cryptides tend to be drawn to the negatively charged membranes of microbial and cancer cells, causing cell death through various mechanisms. This makes them promising candidates for alternative antimicrobial and anti-cancer therapies, as their mechanism of action is independent of gene mutations. In the current study, we employed an in silico approach to identify novel cationic cryptides with potential antimicrobial and anti-cancer activities in atypical and systematic strategy by reanalysis of a publicly available RNA-seq dataset of Pacific white shrimp (Penaus vannamei) in response to bacterial infection. Out of 12 cryptides identified, five were selected based on their net charges and potential for cell penetration. Following chemical synthesis, the cryptides were assayed in vitro to test for their biological activities. All five cryptides demonstrated a wide range of selective activity against the tested microbial and cancer cells, their anti-biofilm activities against mature biofilms, and their ability to interact with Gram-positive and negative bacterial membranes. Our research provides a framework for a comprehensive analysis of transcriptomes in various organisms to uncover novel bioactive cationic cryptides. This represents a significant step forward in combating the crisis of multi-drug-resistant microbial and cancer cells, as these cryptides neither induce mutations nor are influenced by mutations in the cells they target. Nature Publishing Group 2023 Article PeerReviewed text en cc_by_4 http://eprints.sunway.edu.my/2684/1/Kavita%20Reginald_Novel%20cationic%20cryptides%20in%20Penaeus%20vannamei%20demonstrate.pdf Amr Adel, Ahmed Abd El-Aal * and Fairen, Angelin Jayakumar * and Lahiri, Chandrajit * and Tan, Kuan Onn * and Kavita, Reginald * (2023) Novel cationic cryptides in Penaeus vannamei demonstrate antimicrobial and anti-cancer activities. Scientific Reports, 13. ISSN 2045-2322 https://doi.org/10.1038/s41598-023-41581-9 10.1038/s41598-023-41581-9
institution	Sunway University
building	Sunway Campus Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Sunway University
content_source	Sunway Institutional Repository
url_provider	http://eprints.sunway.edu.my/
language	English
topic	QL Zoology RC Internal medicine RM Therapeutics. Pharmacology
spellingShingle	QL Zoology RC Internal medicine RM Therapeutics. Pharmacology Amr Adel, Ahmed Abd El-Aal * Fairen, Angelin Jayakumar * Lahiri, Chandrajit * Tan, Kuan Onn * Kavita, Reginald * Novel cationic cryptides in Penaeus vannamei demonstrate antimicrobial and anti-cancer activities
description	Cryptides are a subfamily of bioactive peptides that exist in all living organisms. They are latently encrypted in their parent sequences and exhibit a wide range of biological activities when decrypted via in vivo or in vitro proteases. Cationic cryptides tend to be drawn to the negatively charged membranes of microbial and cancer cells, causing cell death through various mechanisms. This makes them promising candidates for alternative antimicrobial and anti-cancer therapies, as their mechanism of action is independent of gene mutations. In the current study, we employed an in silico approach to identify novel cationic cryptides with potential antimicrobial and anti-cancer activities in atypical and systematic strategy by reanalysis of a publicly available RNA-seq dataset of Pacific white shrimp (Penaus vannamei) in response to bacterial infection. Out of 12 cryptides identified, five were selected based on their net charges and potential for cell penetration. Following chemical synthesis, the cryptides were assayed in vitro to test for their biological activities. All five cryptides demonstrated a wide range of selective activity against the tested microbial and cancer cells, their anti-biofilm activities against mature biofilms, and their ability to interact with Gram-positive and negative bacterial membranes. Our research provides a framework for a comprehensive analysis of transcriptomes in various organisms to uncover novel bioactive cationic cryptides. This represents a significant step forward in combating the crisis of multi-drug-resistant microbial and cancer cells, as these cryptides neither induce mutations nor are influenced by mutations in the cells they target.
format	Article
author	Amr Adel, Ahmed Abd El-Aal * Fairen, Angelin Jayakumar * Lahiri, Chandrajit * Tan, Kuan Onn * Kavita, Reginald *
author_facet	Amr Adel, Ahmed Abd El-Aal * Fairen, Angelin Jayakumar * Lahiri, Chandrajit * Tan, Kuan Onn * Kavita, Reginald *
author_sort	Amr Adel, Ahmed Abd El-Aal *
title	Novel cationic cryptides in Penaeus vannamei demonstrate antimicrobial and anti-cancer activities
title_short	Novel cationic cryptides in Penaeus vannamei demonstrate antimicrobial and anti-cancer activities
title_full	Novel cationic cryptides in Penaeus vannamei demonstrate antimicrobial and anti-cancer activities
title_fullStr	Novel cationic cryptides in Penaeus vannamei demonstrate antimicrobial and anti-cancer activities
title_full_unstemmed	Novel cationic cryptides in Penaeus vannamei demonstrate antimicrobial and anti-cancer activities
title_sort	novel cationic cryptides in penaeus vannamei demonstrate antimicrobial and anti-cancer activities
publisher	Nature Publishing Group
publishDate	2023
url	http://eprints.sunway.edu.my/2684/1/Kavita%20Reginald_Novel%20cationic%20cryptides%20in%20Penaeus%20vannamei%20demonstrate.pdf http://eprints.sunway.edu.my/2684/ https://doi.org/10.1038/s41598-023-41581-9
_version_	1802980692992196608
score	13.211869

Novel cationic cryptides in Penaeus vannamei demonstrate antimicrobial and anti-cancer activities

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