Staff View: miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia

miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia

Objectives: Despite extensive research in breast cancer (BC) genomics, most studies are from Western countries, which do not reflect the multi-ethnic make-up of Malaysia. Hence, microribonucleic acid-21 (miRNA-21), which is known to be an oncogenic stimulator of BC will be investigated by comparing...

Full description

Saved in:

Bibliographic Details
Main Authors:	Wong, Sharon Rachel, Chong, Pei Pei, Mohd Tamrin, Mohd Islahuddin, Anita, Baghawi, Gew, Lai Ti *, Nallammai, Singaram, Lee, Sau Har
Format:	Article
Published:	Kare Publishing 2024
Subjects:	RC Internal medicine
Online Access:	http://eprints.sunway.edu.my/2630/ https://www.ejmo.org/10.14744/ejmo.2024.24156/
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.sunway.eprints.2630
record_format	eprints
spelling	my.sunway.eprints.26302024-05-20T02:31:22Z http://eprints.sunway.edu.my/2630/ miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia Wong, Sharon Rachel Chong, Pei Pei Mohd Tamrin, Mohd Islahuddin Anita, Baghawi Gew, Lai Ti * Nallammai, Singaram Lee, Sau Har RC Internal medicine Objectives: Despite extensive research in breast cancer (BC) genomics, most studies are from Western countries, which do not reflect the multi-ethnic make-up of Malaysia. Hence, microribonucleic acid-21 (miRNA-21), which is known to be an oncogenic stimulator of BC will be investigated by comparing its expression between breast tumour tissues and normal adjacent tissues excised from 67 BC patients, ethnic groups, age groups distribution, neo-adjuvant chemotherapy (NAC) treated and untreated patients, as well as BC subtypes. Methods: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to measure the distribution of miR-21 expression in the paired BC tissues. The expression of the tumour suppressors; PTEN and PDCD4 was also investigated via RT-qPCR and Western Blot for its gene and protein expressions. Results: The results only showed the significance of miR-21 and PTEN expression between the normal adjacent tissues and BC tissues (p<0.05). Additionally, there was a lack of correlation between gene expression of miR-21 against PTEN and PDCD4. Protein expression analysis did not show a significant difference in tumour suppressor proteins; PTEN and PDCD4 expression in both tissue types. Conclusion: miR-21 has a notable presence in BC and is a suitable biomarker to be evaluated further in patients of all ethnicities and age groups. Kare Publishing 2024 Article PeerReviewed Wong, Sharon Rachel and Chong, Pei Pei and Mohd Tamrin, Mohd Islahuddin and Anita, Baghawi and Gew, Lai Ti * and Nallammai, Singaram and Lee, Sau Har (2024) miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia. Eurasian Journal of Medicine and Oncology, 8 (1). pp. 59-73. ISSN 2587-196X https://www.ejmo.org/10.14744/ejmo.2024.24156/ 10.14744/ejmo.2024.24156
institution	Sunway University
building	Sunway Campus Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Sunway University
content_source	Sunway Institutional Repository
url_provider	http://eprints.sunway.edu.my/
topic	RC Internal medicine
spellingShingle	RC Internal medicine Wong, Sharon Rachel Chong, Pei Pei Mohd Tamrin, Mohd Islahuddin Anita, Baghawi Gew, Lai Ti * Nallammai, Singaram Lee, Sau Har miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia
description	Objectives: Despite extensive research in breast cancer (BC) genomics, most studies are from Western countries, which do not reflect the multi-ethnic make-up of Malaysia. Hence, microribonucleic acid-21 (miRNA-21), which is known to be an oncogenic stimulator of BC will be investigated by comparing its expression between breast tumour tissues and normal adjacent tissues excised from 67 BC patients, ethnic groups, age groups distribution, neo-adjuvant chemotherapy (NAC) treated and untreated patients, as well as BC subtypes. Methods: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to measure the distribution of miR-21 expression in the paired BC tissues. The expression of the tumour suppressors; PTEN and PDCD4 was also investigated via RT-qPCR and Western Blot for its gene and protein expressions. Results: The results only showed the significance of miR-21 and PTEN expression between the normal adjacent tissues and BC tissues (p<0.05). Additionally, there was a lack of correlation between gene expression of miR-21 against PTEN and PDCD4. Protein expression analysis did not show a significant difference in tumour suppressor proteins; PTEN and PDCD4 expression in both tissue types. Conclusion: miR-21 has a notable presence in BC and is a suitable biomarker to be evaluated further in patients of all ethnicities and age groups.
format	Article
author	Wong, Sharon Rachel Chong, Pei Pei Mohd Tamrin, Mohd Islahuddin Anita, Baghawi Gew, Lai Ti * Nallammai, Singaram Lee, Sau Har
author_facet	Wong, Sharon Rachel Chong, Pei Pei Mohd Tamrin, Mohd Islahuddin Anita, Baghawi Gew, Lai Ti * Nallammai, Singaram Lee, Sau Har
author_sort	Wong, Sharon Rachel
title	miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia
title_short	miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia
title_full	miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia
title_fullStr	miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia
title_full_unstemmed	miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia
title_sort	mir-21 expression and its correlation with demographics, subtypes, and tumour suppressor genes; pten and pdcd4 in breast cancer tissues in malaysia
publisher	Kare Publishing
publishDate	2024
url	http://eprints.sunway.edu.my/2630/ https://www.ejmo.org/10.14744/ejmo.2024.24156/
_version_	1800100329399779328
score	13.211869

miR-21 Expression and its Correlation with Demographics, Subtypes, and Tumour Suppressor Genes; PTEN and PDCD4 in Breast Cancer Tissues in Malaysia

Similar Items