Staff View: Biological Degradation of Natural Rubber Glove by Gram-negative Bacteria Klebsiella aerogenes

Biological Degradation of Natural Rubber Glove by Gram-negative Bacteria Klebsiella aerogenes

The increasing urgency and scope of global threat posed by rubber waste have led to efforts to find sustainable and efficient solutions. The biological degradation of rubber waste has gained interest in recent years as an alternative to conventional waste management practices and widely used chemica...

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Main Authors:	Chittella, Harika, Yoon, Li Wan *, Ramarad, Suganti, Lai, Zee-Wei
Format:	Article
Published:	Springer 2024
Subjects:	QH Natural history QP Physiology TD Environmental technology. Sanitary engineering
Online Access:	http://eprints.sunway.edu.my/2629/ https://doi.org/10.21203/rs.3.rs-3368330/v1
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spelling	my.sunway.eprints.26292024-05-20T02:14:40Z http://eprints.sunway.edu.my/2629/ Biological Degradation of Natural Rubber Glove by Gram-negative Bacteria Klebsiella aerogenes Chittella, Harika Yoon, Li Wan * Ramarad, Suganti Lai, Zee-Wei QH Natural history QP Physiology TD Environmental technology. Sanitary engineering The increasing urgency and scope of global threat posed by rubber waste have led to efforts to find sustainable and efficient solutions. The biological degradation of rubber waste has gained interest in recent years as an alternative to conventional waste management practices and widely used chemical methods. This study uses the gram-negative bacterium Klebsiella aerogenes for natural rubber glove (NRG) degradation. The study investigates the effect of temperature and pH on the growth of the bacteria and enzyme production. The biodegraded rubber was characterized by dry weight analysis, growth curve, cell viability, Schiff’s staining, and FTIR analysis. The conversion of rubber waste to enzymes was also investigated in this work. Dry weight analysis showed a maximum weight loss of 17% at pH 7 under 35 °C after 21 days. The growth curve analysis reported a maximum cell biomass of 4.12 g/L during the second week of the biodegradation process. Schiff’s staining analysis showed deep pink colouration, indicating aldehydes and ketone formation. Further confirmation of the presence of new aldehydes and ketones peaks was shown in FTIR analysis. Results also showed that the highest protein concentration of 657.02 µg/mL and enzyme laccase and manganese peroxidase (MnP) activities of 0.43 ± 0.05 IU and 0.26 ± 0.05 IU, respectively, were observed at week 2 of the biodegradation. While Klebsiella aerogenes degrades rubber waste effectively within a shorter duration than other similar studies, laccase and MnP enzymes were also significantly produced during biodegradation. Other aspects of this resource recovery pathway, such as its mechanism and technoeconomic analysis for rubber waste treatment, are worth of further exploring. Springer 2024 Article PeerReviewed Chittella, Harika and Yoon, Li Wan * and Ramarad, Suganti and Lai, Zee-Wei (2024) Biological Degradation of Natural Rubber Glove by Gram-negative Bacteria Klebsiella aerogenes. Environmental Processes, 11 (18). ISSN 2198-7505 https://doi.org/10.21203/rs.3.rs-3368330/v1 10.1007/s40710-024-00695-6
institution	Sunway University
building	Sunway Campus Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Sunway University
content_source	Sunway Institutional Repository
url_provider	http://eprints.sunway.edu.my/
topic	QH Natural history QP Physiology TD Environmental technology. Sanitary engineering
spellingShingle	QH Natural history QP Physiology TD Environmental technology. Sanitary engineering Chittella, Harika Yoon, Li Wan * Ramarad, Suganti Lai, Zee-Wei Biological Degradation of Natural Rubber Glove by Gram-negative Bacteria Klebsiella aerogenes
description	The increasing urgency and scope of global threat posed by rubber waste have led to efforts to find sustainable and efficient solutions. The biological degradation of rubber waste has gained interest in recent years as an alternative to conventional waste management practices and widely used chemical methods. This study uses the gram-negative bacterium Klebsiella aerogenes for natural rubber glove (NRG) degradation. The study investigates the effect of temperature and pH on the growth of the bacteria and enzyme production. The biodegraded rubber was characterized by dry weight analysis, growth curve, cell viability, Schiff’s staining, and FTIR analysis. The conversion of rubber waste to enzymes was also investigated in this work. Dry weight analysis showed a maximum weight loss of 17% at pH 7 under 35 °C after 21 days. The growth curve analysis reported a maximum cell biomass of 4.12 g/L during the second week of the biodegradation process. Schiff’s staining analysis showed deep pink colouration, indicating aldehydes and ketone formation. Further confirmation of the presence of new aldehydes and ketones peaks was shown in FTIR analysis. Results also showed that the highest protein concentration of 657.02 µg/mL and enzyme laccase and manganese peroxidase (MnP) activities of 0.43 ± 0.05 IU and 0.26 ± 0.05 IU, respectively, were observed at week 2 of the biodegradation. While Klebsiella aerogenes degrades rubber waste effectively within a shorter duration than other similar studies, laccase and MnP enzymes were also significantly produced during biodegradation. Other aspects of this resource recovery pathway, such as its mechanism and technoeconomic analysis for rubber waste treatment, are worth of further exploring.
format	Article
author	Chittella, Harika Yoon, Li Wan * Ramarad, Suganti Lai, Zee-Wei
author_facet	Chittella, Harika Yoon, Li Wan * Ramarad, Suganti Lai, Zee-Wei
author_sort	Chittella, Harika
title	Biological Degradation of Natural Rubber Glove by Gram-negative Bacteria Klebsiella aerogenes
title_short	Biological Degradation of Natural Rubber Glove by Gram-negative Bacteria Klebsiella aerogenes
title_full	Biological Degradation of Natural Rubber Glove by Gram-negative Bacteria Klebsiella aerogenes
title_fullStr	Biological Degradation of Natural Rubber Glove by Gram-negative Bacteria Klebsiella aerogenes
title_full_unstemmed	Biological Degradation of Natural Rubber Glove by Gram-negative Bacteria Klebsiella aerogenes
title_sort	biological degradation of natural rubber glove by gram-negative bacteria klebsiella aerogenes
publisher	Springer
publishDate	2024
url	http://eprints.sunway.edu.my/2629/ https://doi.org/10.21203/rs.3.rs-3368330/v1
_version_	1800100329251930112
score	13.211869

Biological Degradation of Natural Rubber Glove by Gram-negative Bacteria Klebsiella aerogenes

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