Staff View: The effect of acute gamma radiation onto growth media for mother culture of a Volvariella mushroom

The effect of acute gamma radiation onto growth media for mother culture of a Volvariella mushroom

Potato Dextrose Agar (PDA) is a semicrystalline polymer made from amylopectin, agarose, and amylose, used in laboratories for Agar plate culturing for a multitude of fungal strains. The existing pretreatment method for the elimination of pathogenic bacteria, namely steam sterilization, has shown sev...

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Main Authors:	Almugren, K.S., Sani, S.F. A., M.S., Jaafar, Azhar, Mohamad, A.S, Siti Shafiqah, Bradley, D.A. *
Format:	Article
Published:	Elsevier 2023
Subjects:	QC Physics SB Plant culture TP Chemical technology
Online Access:	http://eprints.sunway.edu.my/2235/ https://doi.org/10.1016/j.radphyschem.2022.110549
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spelling	my.sunway.eprints.22352023-06-09T05:47:30Z http://eprints.sunway.edu.my/2235/ The effect of acute gamma radiation onto growth media for mother culture of a Volvariella mushroom Almugren, K.S. Sani, S.F. A. M.S., Jaafar Azhar, Mohamad A.S, Siti Shafiqah Bradley, D.A. * QC Physics SB Plant culture TP Chemical technology Potato Dextrose Agar (PDA) is a semicrystalline polymer made from amylopectin, agarose, and amylose, used in laboratories for Agar plate culturing for a multitude of fungal strains. The existing pretreatment method for the elimination of pathogenic bacteria, namely steam sterilization, has shown several drawbacks, including high cost, uneven heating due to temperature differentials and an inability to treat specimens volumetrically. These drawbacks are mitigated by gamma irradiation, being carried out in order to improve hygienic quality and germination control, retarding sprouting, also enhancing physical attributes of the food product. Present study investigates the growth and survival of pretreated potato dextrose agar (PDA) substrate with gamma irradiations, for doses ranging from 0 kGy (unirradiated) up to 20 kGy, particularly for Volvariella mushroom cultivation. The relative elemental composition of PDA substrate has been obtained through use of Energy Dispersive X-ray Analysis (EDX), identifying carbohydrate (53.35%), oxygen (45.29%) and 1.36% of mineral trace elements. Gamma irradiation doses to the PDA substrate of 0.5 kGy, 1 kGy and 1.5 kGy have been found to be associated with respective growth rates of 0.93 ± 0.03, 0.94 ± 0.02 and 0.89 ± 0.02 cm/day, the PDA becoming more amorphous and acidic with reduced viscosity at higher doses. The findings of this study could pave the way for a low-cost yet highly effective system for irradiation in mushroom, as well as offering a viable alternative to current conventional sterilizations, well suited to applications in food security. Elsevier 2023-01 Article PeerReviewed Almugren, K.S. and Sani, S.F. A. and M.S., Jaafar and Azhar, Mohamad and A.S, Siti Shafiqah and Bradley, D.A. * (2023) The effect of acute gamma radiation onto growth media for mother culture of a Volvariella mushroom. Radiation Physics and Chemistry, 202. ISSN 0969-806X https://doi.org/10.1016/j.radphyschem.2022.110549 10.1016/j.radphyschem.2022.110549
institution	Sunway University
building	Sunway Campus Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Sunway University
content_source	Sunway Institutional Repository
url_provider	http://eprints.sunway.edu.my/
topic	QC Physics SB Plant culture TP Chemical technology
spellingShingle	QC Physics SB Plant culture TP Chemical technology Almugren, K.S. Sani, S.F. A. M.S., Jaafar Azhar, Mohamad A.S, Siti Shafiqah Bradley, D.A. * The effect of acute gamma radiation onto growth media for mother culture of a Volvariella mushroom
description	Potato Dextrose Agar (PDA) is a semicrystalline polymer made from amylopectin, agarose, and amylose, used in laboratories for Agar plate culturing for a multitude of fungal strains. The existing pretreatment method for the elimination of pathogenic bacteria, namely steam sterilization, has shown several drawbacks, including high cost, uneven heating due to temperature differentials and an inability to treat specimens volumetrically. These drawbacks are mitigated by gamma irradiation, being carried out in order to improve hygienic quality and germination control, retarding sprouting, also enhancing physical attributes of the food product. Present study investigates the growth and survival of pretreated potato dextrose agar (PDA) substrate with gamma irradiations, for doses ranging from 0 kGy (unirradiated) up to 20 kGy, particularly for Volvariella mushroom cultivation. The relative elemental composition of PDA substrate has been obtained through use of Energy Dispersive X-ray Analysis (EDX), identifying carbohydrate (53.35%), oxygen (45.29%) and 1.36% of mineral trace elements. Gamma irradiation doses to the PDA substrate of 0.5 kGy, 1 kGy and 1.5 kGy have been found to be associated with respective growth rates of 0.93 ± 0.03, 0.94 ± 0.02 and 0.89 ± 0.02 cm/day, the PDA becoming more amorphous and acidic with reduced viscosity at higher doses. The findings of this study could pave the way for a low-cost yet highly effective system for irradiation in mushroom, as well as offering a viable alternative to current conventional sterilizations, well suited to applications in food security.
format	Article
author	Almugren, K.S. Sani, S.F. A. M.S., Jaafar Azhar, Mohamad A.S, Siti Shafiqah Bradley, D.A. *
author_facet	Almugren, K.S. Sani, S.F. A. M.S., Jaafar Azhar, Mohamad A.S, Siti Shafiqah Bradley, D.A. *
author_sort	Almugren, K.S.
title	The effect of acute gamma radiation onto growth media for mother culture of a Volvariella mushroom
title_short	The effect of acute gamma radiation onto growth media for mother culture of a Volvariella mushroom
title_full	The effect of acute gamma radiation onto growth media for mother culture of a Volvariella mushroom
title_fullStr	The effect of acute gamma radiation onto growth media for mother culture of a Volvariella mushroom
title_full_unstemmed	The effect of acute gamma radiation onto growth media for mother culture of a Volvariella mushroom
title_sort	effect of acute gamma radiation onto growth media for mother culture of a volvariella mushroom
publisher	Elsevier
publishDate	2023
url	http://eprints.sunway.edu.my/2235/ https://doi.org/10.1016/j.radphyschem.2022.110549
_version_	1769846263330635776
score	13.211869

The effect of acute gamma radiation onto growth media for mother culture of a Volvariella mushroom

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