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Characterization of plaque variants and the involvement of Quasi-Species in a population of EV-A71

Viral plaque morphologies in human cell lines are markers for growth capability and they have been used to assess the viral fitness and selection of attenuated mutants for live-attenuated vaccine development. In this study, we investigate whether the naturally occurring plaque size variation reflec...

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Main Authors: Mandary, M. B. *, Masomian, Malihe *, Ong, Seng Kai *, Poh, Chit Laa *
Format: Article
Language:English
Published: MDPI 2020
Subjects:
QR355 Virology
Online Access:http://eprints.sunway.edu.my/1320/1/Poh%20Chit%20Laa%20Characterization%20of%20plaque%20variants.pdf
http://eprints.sunway.edu.my/1320/
http://doi.org/10.3390/v12060651
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spelling my.sunway.eprints.13202020-07-02T03:26:09Z http://eprints.sunway.edu.my/1320/ Characterization of plaque variants and the involvement of Quasi-Species in a population of EV-A71 Mandary, M. B. * Masomian, Malihe * Ong, Seng Kai * Poh, Chit Laa * QR355 Virology Viral plaque morphologies in human cell lines are markers for growth capability and they have been used to assess the viral fitness and selection of attenuated mutants for live-attenuated vaccine development. In this study, we investigate whether the naturally occurring plaque size variation reflects the virulence of the variants of EV-A71. Variants of two different plaque sizes (big and small) from EV-A71 sub-genotype B4 strain 41 were characterized. The plaque variants displayed different in vitro growth kinetics compared to the parental wild type. The plaque variants showed specific mutations being present in each variant strain. The big plaque variants showed four mutations I97L, N104S, S246P and N282D in the VP1 while the small plaque variants showed I97T, N237T and T292A in the VP1. No other mutations were detected in the whole genome of the two variants. The variants showed stable homogenous small plaques and big plaques, respectively, when re-infected in rhabdomyosarcoma (RD) and Vero cells. The parental strain showed faster growth kinetics and had higher viral RNA copy number than both the big and small plaque variants. Homology modelling shows that both plaque variants have differences in the structure of the VP1 protein due to the presence of unique spontaneous mutations found in each plaque variant This study suggests that the EV-A71 sub-genotype B4 strain 41 has at least two variants with different plaque morphologies. These differences were likely due to the presence of spontaneous mutations that are unique to each of the plaque variants. The ability to maintain the respective plaque morphology upon passaging indicates the presence of quasi-species in the parental population. MDPI 2020-06-17 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/1320/1/Poh%20Chit%20Laa%20Characterization%20of%20plaque%20variants.pdf Mandary, M. B. * and Masomian, Malihe * and Ong, Seng Kai * and Poh, Chit Laa * (2020) Characterization of plaque variants and the involvement of Quasi-Species in a population of EV-A71. Viruses, 12 (6). p. 651. ISSN 1999-4915 http://doi.org/10.3390/v12060651 doi:10.3390/v12060651
institution Sunway University
building Sunway Campus Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Sunway University
content_source Sunway Institutional Repository
url_provider http://eprints.sunway.edu.my/
language English
topic QR355 Virology
spellingShingle QR355 Virology
Mandary, M. B. *
Masomian, Malihe *
Ong, Seng Kai *
Poh, Chit Laa *
Characterization of plaque variants and the involvement of Quasi-Species in a population of EV-A71
description Viral plaque morphologies in human cell lines are markers for growth capability and they have been used to assess the viral fitness and selection of attenuated mutants for live-attenuated vaccine development. In this study, we investigate whether the naturally occurring plaque size variation reflects the virulence of the variants of EV-A71. Variants of two different plaque sizes (big and small) from EV-A71 sub-genotype B4 strain 41 were characterized. The plaque variants displayed different in vitro growth kinetics compared to the parental wild type. The plaque variants showed specific mutations being present in each variant strain. The big plaque variants showed four mutations I97L, N104S, S246P and N282D in the VP1 while the small plaque variants showed I97T, N237T and T292A in the VP1. No other mutations were detected in the whole genome of the two variants. The variants showed stable homogenous small plaques and big plaques, respectively, when re-infected in rhabdomyosarcoma (RD) and Vero cells. The parental strain showed faster growth kinetics and had higher viral RNA copy number than both the big and small plaque variants. Homology modelling shows that both plaque variants have differences in the structure of the VP1 protein due to the presence of unique spontaneous mutations found in each plaque variant This study suggests that the EV-A71 sub-genotype B4 strain 41 has at least two variants with different plaque morphologies. These differences were likely due to the presence of spontaneous mutations that are unique to each of the plaque variants. The ability to maintain the respective plaque morphology upon passaging indicates the presence of quasi-species in the parental population.
format Article
author Mandary, M. B. *
Masomian, Malihe *
Ong, Seng Kai *
Poh, Chit Laa *
author_facet Mandary, M. B. *
Masomian, Malihe *
Ong, Seng Kai *
Poh, Chit Laa *
author_sort Mandary, M. B. *
title Characterization of plaque variants and the involvement of Quasi-Species in a population of EV-A71
title_short Characterization of plaque variants and the involvement of Quasi-Species in a population of EV-A71
title_full Characterization of plaque variants and the involvement of Quasi-Species in a population of EV-A71
title_fullStr Characterization of plaque variants and the involvement of Quasi-Species in a population of EV-A71
title_full_unstemmed Characterization of plaque variants and the involvement of Quasi-Species in a population of EV-A71
title_sort characterization of plaque variants and the involvement of quasi-species in a population of ev-a71
publisher MDPI
publishDate 2020
url http://eprints.sunway.edu.my/1320/1/Poh%20Chit%20Laa%20Characterization%20of%20plaque%20variants.pdf
http://eprints.sunway.edu.my/1320/
http://doi.org/10.3390/v12060651
_version_ 1672613443589373952
score 13.211869

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