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Optimization of surface sterilization method and initiation of Bitter Cassava Callus Culture

Surface sterilization is essential inexplant preparation for in vitro cultivation to reduce contamination risk. This study focused on optimizing surface sterilizing technique to initiate bitter cassava callus culture (Rayong cultivar)for starch production based on the effects of pl...

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Main Authors: Mohamad Puad, Noor Illi, Mohd Sofri, Nur Syazwani Nadhirah, Amid, Azura, Azmi, Azlin Suhaida
Format: Article
Language:English
Published: IIUM Press 2022
Subjects:
TP Chemical technology
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/98928/1/98928_Optimization%20of%20surface%20sterilization.pdf
http://irep.iium.edu.my/98928/
https://journals.iium.edu.my/bnrej/index.php/bnrej/issue/view/8
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spelling my.iium.irep.989282022-07-27T02:08:36Z http://irep.iium.edu.my/98928/ Optimization of surface sterilization method and initiation of Bitter Cassava Callus Culture Mohamad Puad, Noor Illi Mohd Sofri, Nur Syazwani Nadhirah Amid, Azura Azmi, Azlin Suhaida TP Chemical technology TP248.13 Biotechnology Surface sterilization is essential inexplant preparation for in vitro cultivation to reduce contamination risk. This study focused on optimizing surface sterilizing technique to initiate bitter cassava callus culture (Rayong cultivar)for starch production based on the effects of plant growth regulator (PGR). Bitter cassava is widely used in the food and non-food industries thus, its demand is forecasted to be high in the next several years. Farmers faced many challenges in large-scale cassava plantations such as lack of infrastructure, cassava diseases and poor climatic conditions. Plant tissue culture is proposed due to its advantages such as high yield in a shorter time compared to the traditional method and the cultivation can be done outside the plant’s season. Soaking time in 70% ethanol (min), concentration of sodium hypochlorite (%) and soaking time in sodium hypochlorite (min) were selected for optimization of surface sterilizing condition of cassava explants (leaf and stem). Face Centered Central Composite Design (FCCCD) under Response Surface Methodology (RSM) in Design-Expert v9.0.6.was used for designing experiments and optimization purpose to minimize the percentage of contamination. Next, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (5, 10, 15 and 20 mg/L) were supplemented to Murashige and Skoog (MS) medium to investigate their effect on callus initiation. Based on the results, soaking cassava explants for 5 minutes in 70% ethanol followed by 10 minutes in 5.75% in sodium hypochlorite gave the least percentage of contamination (16.67% for the leaf and 25% for the stem). The highest frequency of callus formation (41.67%) was achieved when using 5 mg/L of 2,4-D from stem explant. The results from this study can serve as a starting point in establishing bitter cassava callus culture for starch production. IIUM Press 2022-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/98928/1/98928_Optimization%20of%20surface%20sterilization.pdf Mohamad Puad, Noor Illi and Mohd Sofri, Nur Syazwani Nadhirah and Amid, Azura and Azmi, Azlin Suhaida (2022) Optimization of surface sterilization method and initiation of Bitter Cassava Callus Culture. Biological And Natural Resources Engineering Journal, 6 (1). pp. 47-59. E-ISSN 2637-0719 https://journals.iium.edu.my/bnrej/index.php/bnrej/issue/view/8
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP Chemical technology
TP248.13 Biotechnology
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Mohamad Puad, Noor Illi
Mohd Sofri, Nur Syazwani Nadhirah
Amid, Azura
Azmi, Azlin Suhaida
Optimization of surface sterilization method and initiation of Bitter Cassava Callus Culture
description Surface sterilization is essential inexplant preparation for in vitro cultivation to reduce contamination risk. This study focused on optimizing surface sterilizing technique to initiate bitter cassava callus culture (Rayong cultivar)for starch production based on the effects of plant growth regulator (PGR). Bitter cassava is widely used in the food and non-food industries thus, its demand is forecasted to be high in the next several years. Farmers faced many challenges in large-scale cassava plantations such as lack of infrastructure, cassava diseases and poor climatic conditions. Plant tissue culture is proposed due to its advantages such as high yield in a shorter time compared to the traditional method and the cultivation can be done outside the plant’s season. Soaking time in 70% ethanol (min), concentration of sodium hypochlorite (%) and soaking time in sodium hypochlorite (min) were selected for optimization of surface sterilizing condition of cassava explants (leaf and stem). Face Centered Central Composite Design (FCCCD) under Response Surface Methodology (RSM) in Design-Expert v9.0.6.was used for designing experiments and optimization purpose to minimize the percentage of contamination. Next, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (5, 10, 15 and 20 mg/L) were supplemented to Murashige and Skoog (MS) medium to investigate their effect on callus initiation. Based on the results, soaking cassava explants for 5 minutes in 70% ethanol followed by 10 minutes in 5.75% in sodium hypochlorite gave the least percentage of contamination (16.67% for the leaf and 25% for the stem). The highest frequency of callus formation (41.67%) was achieved when using 5 mg/L of 2,4-D from stem explant. The results from this study can serve as a starting point in establishing bitter cassava callus culture for starch production.
format Article
author Mohamad Puad, Noor Illi
Mohd Sofri, Nur Syazwani Nadhirah
Amid, Azura
Azmi, Azlin Suhaida
author_facet Mohamad Puad, Noor Illi
Mohd Sofri, Nur Syazwani Nadhirah
Amid, Azura
Azmi, Azlin Suhaida
author_sort Mohamad Puad, Noor Illi
title Optimization of surface sterilization method and initiation of Bitter Cassava Callus Culture
title_short Optimization of surface sterilization method and initiation of Bitter Cassava Callus Culture
title_full Optimization of surface sterilization method and initiation of Bitter Cassava Callus Culture
title_fullStr Optimization of surface sterilization method and initiation of Bitter Cassava Callus Culture
title_full_unstemmed Optimization of surface sterilization method and initiation of Bitter Cassava Callus Culture
title_sort optimization of surface sterilization method and initiation of bitter cassava callus culture
publisher IIUM Press
publishDate 2022
url http://irep.iium.edu.my/98928/1/98928_Optimization%20of%20surface%20sterilization.pdf
http://irep.iium.edu.my/98928/
https://journals.iium.edu.my/bnrej/index.php/bnrej/issue/view/8
_version_ 1739827892755365888
score 13.211869

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