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MDMA-induced BV2 microglial cell activation in vitro

Background: 3,4-Methylenedioxymethamphetamine (MDMA) is a psychostimulant drug that induces neurotoxicity. Even though several psychostimulant substances activate microglia, little is known about MDMA's effects on these cells, and evidence of MDMA-induced microglial activation is equivocal....

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Main Authors: Mohamad, Nasir, Mustafa, Nor Suliana, Abu Bakar, Nor Hidayah, Musa, Ramli, Mohd Adnan, Liyana Hazwani, Ahmad, Nor Zidah, Mohd Daud, Mohd Nazri, Mohamed Hashim, Zulfa Izza
Format: Article
Language:English
Published: Shahid Beheshti University of Medical Sciences 2022
Subjects:
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Online Access:http://irep.iium.edu.my/98717/7/98717_MDMA-Induced%20BV2%20microglial%20cell%20activation%20in%20vitro.pdf
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spelling my.iium.irep.987172022-07-07T04:43:17Z http://irep.iium.edu.my/98717/ MDMA-induced BV2 microglial cell activation in vitro Mohamad, Nasir Mustafa, Nor Suliana Abu Bakar, Nor Hidayah Musa, Ramli Mohd Adnan, Liyana Hazwani Ahmad, Nor Zidah Mohd Daud, Mohd Nazri Mohamed Hashim, Zulfa Izza RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Background: 3,4-Methylenedioxymethamphetamine (MDMA) is a psychostimulant drug that induces neurotoxicity. Even though several psychostimulant substances activate microglia, little is known about MDMA's effects on these cells, and evidence of MDMA-induced microglial activation is equivocal. Materials and Methods: This study employed a murine microglial cell line, BV2, to examine the effects of MDMA on the microglia morphological changes and the survival of microglia in vitro. MDMA was incorporated into the media at the time of plating, and cell number and mitochondrial dehydrogenase activity (MTT) levels were determined in vitro. The level of pro-inflammatory cytokine TNF-α was also determined. Results: Treatment of BV2 cells with MDMA resulted in morphological changes, reduced cell viability after 24h incubation with the inhibitory concentration (IC50) value of 243.6 µg/mL, and increased TNF-α level in a dose-dependent manner. Conclusion: These findings proposed that MDMA could induce BV2 microglial cell activation in vitro and suggested that it has an essential role in developing MDMA use disorder. Shahid Beheshti University of Medical Sciences 2022 Article PeerReviewed application/pdf en http://irep.iium.edu.my/98717/7/98717_MDMA-Induced%20BV2%20microglial%20cell%20activation%20in%20vitro.pdf Mohamad, Nasir and Mustafa, Nor Suliana and Abu Bakar, Nor Hidayah and Musa, Ramli and Mohd Adnan, Liyana Hazwani and Ahmad, Nor Zidah and Mohd Daud, Mohd Nazri and Mohamed Hashim, Zulfa Izza (2022) MDMA-induced BV2 microglial cell activation in vitro. Journal of Cellular & Molecular Anesthesia (JCMA)., 7 (3). pp. 139-146. ISSN 2538-2462 E-ISSN 2476-5120 10.22037/jcma.v7i3.38360
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
spellingShingle RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Mohamad, Nasir
Mustafa, Nor Suliana
Abu Bakar, Nor Hidayah
Musa, Ramli
Mohd Adnan, Liyana Hazwani
Ahmad, Nor Zidah
Mohd Daud, Mohd Nazri
Mohamed Hashim, Zulfa Izza
MDMA-induced BV2 microglial cell activation in vitro
description Background: 3,4-Methylenedioxymethamphetamine (MDMA) is a psychostimulant drug that induces neurotoxicity. Even though several psychostimulant substances activate microglia, little is known about MDMA's effects on these cells, and evidence of MDMA-induced microglial activation is equivocal. Materials and Methods: This study employed a murine microglial cell line, BV2, to examine the effects of MDMA on the microglia morphological changes and the survival of microglia in vitro. MDMA was incorporated into the media at the time of plating, and cell number and mitochondrial dehydrogenase activity (MTT) levels were determined in vitro. The level of pro-inflammatory cytokine TNF-α was also determined. Results: Treatment of BV2 cells with MDMA resulted in morphological changes, reduced cell viability after 24h incubation with the inhibitory concentration (IC50) value of 243.6 µg/mL, and increased TNF-α level in a dose-dependent manner. Conclusion: These findings proposed that MDMA could induce BV2 microglial cell activation in vitro and suggested that it has an essential role in developing MDMA use disorder.
format Article
author Mohamad, Nasir
Mustafa, Nor Suliana
Abu Bakar, Nor Hidayah
Musa, Ramli
Mohd Adnan, Liyana Hazwani
Ahmad, Nor Zidah
Mohd Daud, Mohd Nazri
Mohamed Hashim, Zulfa Izza
author_facet Mohamad, Nasir
Mustafa, Nor Suliana
Abu Bakar, Nor Hidayah
Musa, Ramli
Mohd Adnan, Liyana Hazwani
Ahmad, Nor Zidah
Mohd Daud, Mohd Nazri
Mohamed Hashim, Zulfa Izza
author_sort Mohamad, Nasir
title MDMA-induced BV2 microglial cell activation in vitro
title_short MDMA-induced BV2 microglial cell activation in vitro
title_full MDMA-induced BV2 microglial cell activation in vitro
title_fullStr MDMA-induced BV2 microglial cell activation in vitro
title_full_unstemmed MDMA-induced BV2 microglial cell activation in vitro
title_sort mdma-induced bv2 microglial cell activation in vitro
publisher Shahid Beheshti University of Medical Sciences
publishDate 2022
url http://irep.iium.edu.my/98717/7/98717_MDMA-Induced%20BV2%20microglial%20cell%20activation%20in%20vitro.pdf
http://irep.iium.edu.my/98717/
_version_ 1738510130771656704
score 13.211869

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