Staff View: Phytochemical constituents of aquilaria malaccensis leaf extract and their anti-Inflammatory activity against LPS/IFN-γ stimulated RAW 264.7 cell line

Phytochemical constituents of aquilaria malaccensis leaf extract and their anti-Inflammatory activity against LPS/IFN-γ stimulated RAW 264.7 cell line

This study aims to identify the major phytochemical constituents in Aquilaria malaccensis (Thymelaeaceae) ethanolic leaf extract (ALEX-M) and elucidate their ability to suppress nitric oxide (NO) production from a murine macrophage-like cell line (RAW 264.7) stimulated by lipopolysaccharide (LPS) an...

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Main Authors:	Eissa, Manar A., Hashim, Yumi Zuhanis Has-Yun, Syed Abdul Azziz, Saripah Salbiah, Mohd. Salleh, Hamzah, Md Isa, Muhammad Lokman, Abd Warif, Nor Malia, Abdullah, Fauziah, Ramadan, Eman, El-Kersh, Dina
Format:	Article
Language:	English
Published:	ACS Publication 2022
Subjects:	Q Science (General) QD Chemistry QK Botany R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/97814/1/acsomega.2c00439.pdf http://irep.iium.edu.my/97814/ https://pubs.acs.org/
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spelling	my.iium.irep.978142022-05-10T07:26:38Z http://irep.iium.edu.my/97814/ Phytochemical constituents of aquilaria malaccensis leaf extract and their anti-Inflammatory activity against LPS/IFN-γ stimulated RAW 264.7 cell line Eissa, Manar A. Hashim, Yumi Zuhanis Has-Yun Syed Abdul Azziz, Saripah Salbiah Mohd. Salleh, Hamzah Md Isa, Muhammad Lokman Abd Warif, Nor Malia Abdullah, Fauziah Ramadan, Eman El-Kersh, Dina Q Science (General) QD Chemistry QK Botany R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica This study aims to identify the major phytochemical constituents in Aquilaria malaccensis (Thymelaeaceae) ethanolic leaf extract (ALEX-M) and elucidate their ability to suppress nitric oxide (NO) production from a murine macrophage-like cell line (RAW 264.7) stimulated by lipopolysaccharide (LPS) and interferon-γ (IFN-γ). Dichloromethane (DCM) and ethyl acetate (EtOAc) fractions of ALEX-M were subjected to column chromatography. Eight known compounds were isolated for the first time from this species. Compounds were identified using spectroscopic techniques (IR, UV, HRESIMS, and 1D and 2D NMR). Anti-inflammatory activity of both extract and isolated compounds were investigated in vitro. The fractions offered the isolation of epifriedelanol (1), 5-hydroxy-7,4′-dimethoxyflavone (2), luteolin-7,3′,4′-trimethyl ether (3), luteolin-7,4′-dimethyl ether (4), acacetin (5), aquilarinenside E (6), iriflophenone-2-O-α-l-rhamnopyranoside (7), and iriflophenone-3-C-β-glucoside (8). The findings suggest the pharmacological potential of the crude extract (ALEX-M) and its isolates as natural anti-inflammatory agents, capable of suppressing NO production in RAW 264.7 cells stimulated by LPS/IFN-γ. ACS Publication 2022-04-13 Article PeerReviewed application/pdf en http://irep.iium.edu.my/97814/1/acsomega.2c00439.pdf Eissa, Manar A. and Hashim, Yumi Zuhanis Has-Yun and Syed Abdul Azziz, Saripah Salbiah and Mohd. Salleh, Hamzah and Md Isa, Muhammad Lokman and Abd Warif, Nor Malia and Abdullah, Fauziah and Ramadan, Eman and El-Kersh, Dina (2022) Phytochemical constituents of aquilaria malaccensis leaf extract and their anti-Inflammatory activity against LPS/IFN-γ stimulated RAW 264.7 cell line. ACS Omega. ISSN 2470-1343 (In Press) https://pubs.acs.org/
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	Q Science (General) QD Chemistry QK Botany R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica
spellingShingle	Q Science (General) QD Chemistry QK Botany R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica Eissa, Manar A. Hashim, Yumi Zuhanis Has-Yun Syed Abdul Azziz, Saripah Salbiah Mohd. Salleh, Hamzah Md Isa, Muhammad Lokman Abd Warif, Nor Malia Abdullah, Fauziah Ramadan, Eman El-Kersh, Dina Phytochemical constituents of aquilaria malaccensis leaf extract and their anti-Inflammatory activity against LPS/IFN-γ stimulated RAW 264.7 cell line
description	This study aims to identify the major phytochemical constituents in Aquilaria malaccensis (Thymelaeaceae) ethanolic leaf extract (ALEX-M) and elucidate their ability to suppress nitric oxide (NO) production from a murine macrophage-like cell line (RAW 264.7) stimulated by lipopolysaccharide (LPS) and interferon-γ (IFN-γ). Dichloromethane (DCM) and ethyl acetate (EtOAc) fractions of ALEX-M were subjected to column chromatography. Eight known compounds were isolated for the first time from this species. Compounds were identified using spectroscopic techniques (IR, UV, HRESIMS, and 1D and 2D NMR). Anti-inflammatory activity of both extract and isolated compounds were investigated in vitro. The fractions offered the isolation of epifriedelanol (1), 5-hydroxy-7,4′-dimethoxyflavone (2), luteolin-7,3′,4′-trimethyl ether (3), luteolin-7,4′-dimethyl ether (4), acacetin (5), aquilarinenside E (6), iriflophenone-2-O-α-l-rhamnopyranoside (7), and iriflophenone-3-C-β-glucoside (8). The findings suggest the pharmacological potential of the crude extract (ALEX-M) and its isolates as natural anti-inflammatory agents, capable of suppressing NO production in RAW 264.7 cells stimulated by LPS/IFN-γ.
format	Article
author	Eissa, Manar A. Hashim, Yumi Zuhanis Has-Yun Syed Abdul Azziz, Saripah Salbiah Mohd. Salleh, Hamzah Md Isa, Muhammad Lokman Abd Warif, Nor Malia Abdullah, Fauziah Ramadan, Eman El-Kersh, Dina
author_facet	Eissa, Manar A. Hashim, Yumi Zuhanis Has-Yun Syed Abdul Azziz, Saripah Salbiah Mohd. Salleh, Hamzah Md Isa, Muhammad Lokman Abd Warif, Nor Malia Abdullah, Fauziah Ramadan, Eman El-Kersh, Dina
author_sort	Eissa, Manar A.
title	Phytochemical constituents of aquilaria malaccensis leaf extract and their anti-Inflammatory activity against LPS/IFN-γ stimulated RAW 264.7 cell line
title_short	Phytochemical constituents of aquilaria malaccensis leaf extract and their anti-Inflammatory activity against LPS/IFN-γ stimulated RAW 264.7 cell line
title_full	Phytochemical constituents of aquilaria malaccensis leaf extract and their anti-Inflammatory activity against LPS/IFN-γ stimulated RAW 264.7 cell line
title_fullStr	Phytochemical constituents of aquilaria malaccensis leaf extract and their anti-Inflammatory activity against LPS/IFN-γ stimulated RAW 264.7 cell line
title_full_unstemmed	Phytochemical constituents of aquilaria malaccensis leaf extract and their anti-Inflammatory activity against LPS/IFN-γ stimulated RAW 264.7 cell line
title_sort	phytochemical constituents of aquilaria malaccensis leaf extract and their anti-inflammatory activity against lps/ifn-γ stimulated raw 264.7 cell line
publisher	ACS Publication
publishDate	2022
url	http://irep.iium.edu.my/97814/1/acsomega.2c00439.pdf http://irep.iium.edu.my/97814/ https://pubs.acs.org/
_version_	1732945018392412160
score	13.211869

Phytochemical constituents of aquilaria malaccensis leaf extract and their anti-Inflammatory activity against LPS/IFN-γ stimulated RAW 264.7 cell line

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