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Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design

The gene encoding for phytase from ASUIA279 has been successfully cloned and expressed in pichia pastoris X-33 under the control of AOX1 promoter, named as HSUIA279(5H)AX. In this study, process and media components were screened for high yield phytase production in small scale culture by using plac...

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Main Authors: Mahamad Maifiah, Mohd Hafidz, Mohd. Salleh, Hamzah, Jamal, Parveen, Nuge, Tamrin
Format: Conference or Workshop Item
Language:English
Published: 2011
Subjects:
TA164 Bioengineering
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/9779/1/hafidz.pdf
http://irep.iium.edu.my/9779/
http://www.iium.edu.my/icbioe/2011/
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spelling my.iium.irep.97792021-06-23T06:49:44Z http://irep.iium.edu.my/9779/ Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design Mahamad Maifiah, Mohd Hafidz Mohd. Salleh, Hamzah Jamal, Parveen Nuge, Tamrin TA164 Bioengineering TP248.13 Biotechnology The gene encoding for phytase from ASUIA279 has been successfully cloned and expressed in pichia pastoris X-33 under the control of AOX1 promoter, named as HSUIA279(5H)AX. In this study, process and media components were screened for high yield phytase production in small scale culture by using plackett burman design. Three factors identified (Agitation, YNB, Yeast extract) were further optimized by Response Surface methodology (RSM) using Face Centered Composite Design (FCCD). Phytase activity in the optimized media (28 U/ml) increase 64% higher over than it basal media (17.004 U/ml) in the shaken flask culture. The cloning of the phytase enzyme from ASUIA279 into the Pichia pastoris system with minimal and potential media components would be a significant concern for a cost effective measure to produce a high yield and activity enzyme for animal feed application. 2011 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/9779/1/hafidz.pdf Mahamad Maifiah, Mohd Hafidz and Mohd. Salleh, Hamzah and Jamal, Parveen and Nuge, Tamrin (2011) Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TA164 Bioengineering
TP248.13 Biotechnology
spellingShingle TA164 Bioengineering
TP248.13 Biotechnology
Mahamad Maifiah, Mohd Hafidz
Mohd. Salleh, Hamzah
Jamal, Parveen
Nuge, Tamrin
Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
description The gene encoding for phytase from ASUIA279 has been successfully cloned and expressed in pichia pastoris X-33 under the control of AOX1 promoter, named as HSUIA279(5H)AX. In this study, process and media components were screened for high yield phytase production in small scale culture by using plackett burman design. Three factors identified (Agitation, YNB, Yeast extract) were further optimized by Response Surface methodology (RSM) using Face Centered Composite Design (FCCD). Phytase activity in the optimized media (28 U/ml) increase 64% higher over than it basal media (17.004 U/ml) in the shaken flask culture. The cloning of the phytase enzyme from ASUIA279 into the Pichia pastoris system with minimal and potential media components would be a significant concern for a cost effective measure to produce a high yield and activity enzyme for animal feed application.
format Conference or Workshop Item
author Mahamad Maifiah, Mohd Hafidz
Mohd. Salleh, Hamzah
Jamal, Parveen
Nuge, Tamrin
author_facet Mahamad Maifiah, Mohd Hafidz
Mohd. Salleh, Hamzah
Jamal, Parveen
Nuge, Tamrin
author_sort Mahamad Maifiah, Mohd Hafidz
title Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
title_short Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
title_full Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
title_fullStr Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
title_full_unstemmed Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
title_sort screening and optimization of media components for phytase production of asuia 279 in pichia pastoris system using statistical design
publishDate 2011
url http://irep.iium.edu.my/9779/1/hafidz.pdf
http://irep.iium.edu.my/9779/
http://www.iium.edu.my/icbioe/2011/
_version_ 1703960127774654464
score 13.211869

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