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Bacterial metabolomics: sample preparation methods

Metabolomics is a comprehensive analysis of metabolites existing in biological systems. As one of the important “omics” tools, the approach has been widely employed in various fields in helping to better understand the complex cellular metabolic states and changes. Bacterial metabolomics has gained...

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Main Authors: Khairunnisa, Mohd Kamal, Mahamad Maifiah, Mohd Hafidz, Abdul Rahim, Nusaibah, Hashim, Yumi Zuhanis Has-Yun, Abdullah Sani, Muhamad Shirwan, Azizan, Kamalrul Azlan
Format: Article
Language:English
Published: HINDAWI 2022
Subjects:
QD Chemistry
QR Microbiology
TP Chemical technology
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/97665/7/97665_Bacterial%20metabolomics_sample%20preparation%20methods.pdf
http://irep.iium.edu.my/97665/
https://www.hindawi.com/journals/bri/2022/9186536/
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spelling my.iium.irep.976652022-04-18T00:47:02Z http://irep.iium.edu.my/97665/ Bacterial metabolomics: sample preparation methods Khairunnisa, Mohd Kamal Mahamad Maifiah, Mohd Hafidz Abdul Rahim, Nusaibah Hashim, Yumi Zuhanis Has-Yun Abdullah Sani, Muhamad Shirwan Azizan, Kamalrul Azlan QD Chemistry QR Microbiology TP Chemical technology TP248.13 Biotechnology Metabolomics is a comprehensive analysis of metabolites existing in biological systems. As one of the important “omics” tools, the approach has been widely employed in various fields in helping to better understand the complex cellular metabolic states and changes. Bacterial metabolomics has gained a significant interest as bacteria serve to provide a better subject or model at systems level. ,e approach in metabolomics is categorized into untargeted and targeted which serves different paradigms of interest. Nevertheless, the bottleneck in metabolomics has been the sample or metabolite preparation method. A custom-made method and design for a particular species or strain of bacteria might be necessary as most studies generally refer to other bacteria or even yeast and fungi that may lead to unreliable analysis. ,e paramount aspect of metabolomics design comprises sample harvesting, quenching, and metabolite extraction procedures. Depending on the type of samples and research objective, each step must be at optimal conditions which are significantly important in determining the final output. To date, there are no standardized nor single designated protocols that have been established for a specific bacteria strain for untargeted and targeted approaches. In this paper, the existing and current developments of sample preparation methods of bacterial metabolomics used in both approaches are reviewed. ,e review also highlights previous literature of optimized conditions used to propose the most ideal methods for metabolite preparation, particularly for bacterial cells. Advantages and limitations of methods are discussed for future improvement of bacterial metabolomics. HINDAWI 2022-04-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/97665/7/97665_Bacterial%20metabolomics_sample%20preparation%20methods.pdf Khairunnisa, Mohd Kamal and Mahamad Maifiah, Mohd Hafidz and Abdul Rahim, Nusaibah and Hashim, Yumi Zuhanis Has-Yun and Abdullah Sani, Muhamad Shirwan and Azizan, Kamalrul Azlan (2022) Bacterial metabolomics: sample preparation methods. Biochemistry Research International, 2022 (9186536). pp. 1-14. ISSN 2090-2247 E-ISSN 2090-2255 https://www.hindawi.com/journals/bri/2022/9186536/ 10.1155/2022/9186536
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QD Chemistry
QR Microbiology
TP Chemical technology
TP248.13 Biotechnology
spellingShingle QD Chemistry
QR Microbiology
TP Chemical technology
TP248.13 Biotechnology
Khairunnisa, Mohd Kamal
Mahamad Maifiah, Mohd Hafidz
Abdul Rahim, Nusaibah
Hashim, Yumi Zuhanis Has-Yun
Abdullah Sani, Muhamad Shirwan
Azizan, Kamalrul Azlan
Bacterial metabolomics: sample preparation methods
description Metabolomics is a comprehensive analysis of metabolites existing in biological systems. As one of the important “omics” tools, the approach has been widely employed in various fields in helping to better understand the complex cellular metabolic states and changes. Bacterial metabolomics has gained a significant interest as bacteria serve to provide a better subject or model at systems level. ,e approach in metabolomics is categorized into untargeted and targeted which serves different paradigms of interest. Nevertheless, the bottleneck in metabolomics has been the sample or metabolite preparation method. A custom-made method and design for a particular species or strain of bacteria might be necessary as most studies generally refer to other bacteria or even yeast and fungi that may lead to unreliable analysis. ,e paramount aspect of metabolomics design comprises sample harvesting, quenching, and metabolite extraction procedures. Depending on the type of samples and research objective, each step must be at optimal conditions which are significantly important in determining the final output. To date, there are no standardized nor single designated protocols that have been established for a specific bacteria strain for untargeted and targeted approaches. In this paper, the existing and current developments of sample preparation methods of bacterial metabolomics used in both approaches are reviewed. ,e review also highlights previous literature of optimized conditions used to propose the most ideal methods for metabolite preparation, particularly for bacterial cells. Advantages and limitations of methods are discussed for future improvement of bacterial metabolomics.
format Article
author Khairunnisa, Mohd Kamal
Mahamad Maifiah, Mohd Hafidz
Abdul Rahim, Nusaibah
Hashim, Yumi Zuhanis Has-Yun
Abdullah Sani, Muhamad Shirwan
Azizan, Kamalrul Azlan
author_facet Khairunnisa, Mohd Kamal
Mahamad Maifiah, Mohd Hafidz
Abdul Rahim, Nusaibah
Hashim, Yumi Zuhanis Has-Yun
Abdullah Sani, Muhamad Shirwan
Azizan, Kamalrul Azlan
author_sort Khairunnisa, Mohd Kamal
title Bacterial metabolomics: sample preparation methods
title_short Bacterial metabolomics: sample preparation methods
title_full Bacterial metabolomics: sample preparation methods
title_fullStr Bacterial metabolomics: sample preparation methods
title_full_unstemmed Bacterial metabolomics: sample preparation methods
title_sort bacterial metabolomics: sample preparation methods
publisher HINDAWI
publishDate 2022
url http://irep.iium.edu.my/97665/7/97665_Bacterial%20metabolomics_sample%20preparation%20methods.pdf
http://irep.iium.edu.my/97665/
https://www.hindawi.com/journals/bri/2022/9186536/
_version_ 1731225382118490112
score 13.211869

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