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Ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy

Background: Chemotherapy is commonly used in oral cancer therapy, especially as the disease advances. However, it is associated with terrible adverse effects and the occurrence of chemoresistance which causes treatment failure. Thus, discovering a new potential anticancer agent and developing a safe...

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Main Authors: Mohammad Hamdi, Nurul Ain, Harun Ismail, Ahmad Fahmi, Haris @ Harith, Muhammad Salahuddin, Lestari, Widya
Format: Article
Language:English
English
Published: Faculty of Pharmaceutical Sciences, Chulalongkorn University 2023
Subjects:
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/97489/2/Accepted%20Email.pdf
http://irep.iium.edu.my/97489/19/97489_Ascorbic%20acid-loaded%20poly%28lactic-co-glycolic%20acid%29%20nanoparticles.pdf
http://irep.iium.edu.my/97489/
https://digital.car.chula.ac.th/tjps/vol46/iss6/10?utm_source=digital.car.chula.ac.th%2Ftjps%2Fvol46%2Fiss6%2F10&utm_medium=PDF&utm_campaign=PDFCoverPages
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spelling my.iium.irep.974892023-09-04T07:25:35Z http://irep.iium.edu.my/97489/ Ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy Mohammad Hamdi, Nurul Ain Harun Ismail, Ahmad Fahmi Haris @ Harith, Muhammad Salahuddin Lestari, Widya RS Pharmacy and materia medica Background: Chemotherapy is commonly used in oral cancer therapy, especially as the disease advances. However, it is associated with terrible adverse effects and the occurrence of chemoresistance which causes treatment failure. Thus, discovering a new potential anticancer agent and developing a safe, effective and non-invasive drug delivery are necessary. Objective: The objective of the current study is to develop ascorbic acid-loaded poly(lactic-co-glycolic) acid (AA-PLGA) nanoparticles incorporated into polyacrylic acid gel intended to treat oral cancer. Materials and methods: Double emulsion solvent evaporation method was used to fabricate AA-PLGA nanoparticles. Optimisation was carried out in the primary emulsion based on multilevel factorial design by testing at varying surfactant types and concentrations. The optimised nanoparticles formulation was further incorporated into different concentrations of polyacrylic acid gel, and compared with a mucoadhesive polyacrylic acid-based commercial product (Kin Care) as a reference. The optimised AA-PLGA nanoparticles were subjected to cytotoxic assay against the SCC-25 cell line. Results: For the optimised formulation, we observed particle size of 252 ± 2.98 nm, polydispersity index (PDI) of 0.151 ± 0.02, zeta potential of -20.93 ± 0.87 mV, and encapsulation efficiency of 69.73 ± 1.07%. Polyacrylic acid polymer with a strength of 1% was chosen as the optimum gelling agent for AA-PLGA nanoparticles-in-gel formulation. Cytotoxicity study of the optimised nanoparticle demonstrated significant (p-value < 0.05) reduction of cancer cell viability in a dose-dependent manner with a half-maximal inhibitory concentration value of 2.42 mg/mL. Conclusion: The results of the present study support the feasibility of AA-PLGA nanoparticles-in-gel formulation for oral cancer therapy. Faculty of Pharmaceutical Sciences, Chulalongkorn University 2023-06-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/97489/2/Accepted%20Email.pdf application/pdf en http://irep.iium.edu.my/97489/19/97489_Ascorbic%20acid-loaded%20poly%28lactic-co-glycolic%20acid%29%20nanoparticles.pdf Mohammad Hamdi, Nurul Ain and Harun Ismail, Ahmad Fahmi and Haris @ Harith, Muhammad Salahuddin and Lestari, Widya (2023) Ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy. Thai Journal of Pharmaceutical Sciences, 46 (6). pp. 696-710. ISSN 0125-4685 E-ISSN 1905-4637 https://digital.car.chula.ac.th/tjps/vol46/iss6/10?utm_source=digital.car.chula.ac.th%2Ftjps%2Fvol46%2Fiss6%2F10&utm_medium=PDF&utm_campaign=PDFCoverPages
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Mohammad Hamdi, Nurul Ain
Harun Ismail, Ahmad Fahmi
Haris @ Harith, Muhammad Salahuddin
Lestari, Widya
Ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy
description Background: Chemotherapy is commonly used in oral cancer therapy, especially as the disease advances. However, it is associated with terrible adverse effects and the occurrence of chemoresistance which causes treatment failure. Thus, discovering a new potential anticancer agent and developing a safe, effective and non-invasive drug delivery are necessary. Objective: The objective of the current study is to develop ascorbic acid-loaded poly(lactic-co-glycolic) acid (AA-PLGA) nanoparticles incorporated into polyacrylic acid gel intended to treat oral cancer. Materials and methods: Double emulsion solvent evaporation method was used to fabricate AA-PLGA nanoparticles. Optimisation was carried out in the primary emulsion based on multilevel factorial design by testing at varying surfactant types and concentrations. The optimised nanoparticles formulation was further incorporated into different concentrations of polyacrylic acid gel, and compared with a mucoadhesive polyacrylic acid-based commercial product (Kin Care) as a reference. The optimised AA-PLGA nanoparticles were subjected to cytotoxic assay against the SCC-25 cell line. Results: For the optimised formulation, we observed particle size of 252 ± 2.98 nm, polydispersity index (PDI) of 0.151 ± 0.02, zeta potential of -20.93 ± 0.87 mV, and encapsulation efficiency of 69.73 ± 1.07%. Polyacrylic acid polymer with a strength of 1% was chosen as the optimum gelling agent for AA-PLGA nanoparticles-in-gel formulation. Cytotoxicity study of the optimised nanoparticle demonstrated significant (p-value < 0.05) reduction of cancer cell viability in a dose-dependent manner with a half-maximal inhibitory concentration value of 2.42 mg/mL. Conclusion: The results of the present study support the feasibility of AA-PLGA nanoparticles-in-gel formulation for oral cancer therapy.
format Article
author Mohammad Hamdi, Nurul Ain
Harun Ismail, Ahmad Fahmi
Haris @ Harith, Muhammad Salahuddin
Lestari, Widya
author_facet Mohammad Hamdi, Nurul Ain
Harun Ismail, Ahmad Fahmi
Haris @ Harith, Muhammad Salahuddin
Lestari, Widya
author_sort Mohammad Hamdi, Nurul Ain
title Ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy
title_short Ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy
title_full Ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy
title_fullStr Ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy
title_full_unstemmed Ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy
title_sort ascorbic acid-loaded poly(lactic-co-glycolic acid) nanoparticles incorporated into a polyacrylic acid gel as a promising tool for site-specific oral cancer therapy
publisher Faculty of Pharmaceutical Sciences, Chulalongkorn University
publishDate 2023
url http://irep.iium.edu.my/97489/2/Accepted%20Email.pdf
http://irep.iium.edu.my/97489/19/97489_Ascorbic%20acid-loaded%20poly%28lactic-co-glycolic%20acid%29%20nanoparticles.pdf
http://irep.iium.edu.my/97489/
https://digital.car.chula.ac.th/tjps/vol46/iss6/10?utm_source=digital.car.chula.ac.th%2Ftjps%2Fvol46%2Fiss6%2F10&utm_medium=PDF&utm_campaign=PDFCoverPages
_version_ 1776246850800582656
score 13.211869

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