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3D culture of cancer cells in alginate hydrogel beads as an effective technique for emergency cell storage and transportation in the pandemic era

Due to the restrictions in accessing research laboratories and the challenges in providing proper storage and transportation of cells during the COVID-19 pandemic, having an effective and feasible mean to solve these challenges would be of immense help. Therefore, we developed a 3D culture setting o...

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Main Authors: Alallam, Batoul, Oo, May Kyaw, Ibrahim, Wisam Nabeel, Doolaanea, Abd Almonem
Format: Article
Language:English
Published: Wiley 2021
Subjects:
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/95076/13/95076_3D%20culture%20of%20cancer%20cells%20in%20alginate%20hydrogel%20beads%20as.pdf
http://irep.iium.edu.my/95076/
http://wileyonlinelibrary.com/journal/jcmm
https://doi.org/10.1111/jcmm.17078
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spelling my.iium.irep.950762021-12-28T08:56:28Z http://irep.iium.edu.my/95076/ 3D culture of cancer cells in alginate hydrogel beads as an effective technique for emergency cell storage and transportation in the pandemic era Alallam, Batoul Oo, May Kyaw Ibrahim, Wisam Nabeel Doolaanea, Abd Almonem RS Pharmacy and materia medica Due to the restrictions in accessing research laboratories and the challenges in providing proper storage and transportation of cells during the COVID-19 pandemic, having an effective and feasible mean to solve these challenges would be of immense help. Therefore, we developed a 3D culture setting of cancer cells using alginate beads and tested its effectiveness in different storage and transportation conditions. The viability and proliferation of cancer cells were assessed using trypan blue staining and quantitative CCK-8 kit, respectively. The developed beads allowed cancer cells survival up to 4 weeks with less frequent maintenance measures such as change of the culture media or subculture of cells. In addition, the recovery of cancer cells and proliferation pattern were significantly faster with better outcomes in the developed 3D alginate beads compared to the standard cryopreservation of cells or the 2D culture conditions. The 3D alginate beads also supported the viability of cells while the shipment at room temperature for a duration of up to 5 days with no humidity or CO2 support. Therefore, 3D culture in alginate beads can be used to store or ship biological cells with ease at room temperature with minimal preparations. Wiley 2021-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/95076/13/95076_3D%20culture%20of%20cancer%20cells%20in%20alginate%20hydrogel%20beads%20as.pdf Alallam, Batoul and Oo, May Kyaw and Ibrahim, Wisam Nabeel and Doolaanea, Abd Almonem (2021) 3D culture of cancer cells in alginate hydrogel beads as an effective technique for emergency cell storage and transportation in the pandemic era. Journal of Cellular and Molecular Medicine. pp. 1-4. ISSN 1582-1838 E-ISSN 1582-4934 (In Press) http://wileyonlinelibrary.com/journal/jcmm https://doi.org/10.1111/jcmm.17078
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Alallam, Batoul
Oo, May Kyaw
Ibrahim, Wisam Nabeel
Doolaanea, Abd Almonem
3D culture of cancer cells in alginate hydrogel beads as an effective technique for emergency cell storage and transportation in the pandemic era
description Due to the restrictions in accessing research laboratories and the challenges in providing proper storage and transportation of cells during the COVID-19 pandemic, having an effective and feasible mean to solve these challenges would be of immense help. Therefore, we developed a 3D culture setting of cancer cells using alginate beads and tested its effectiveness in different storage and transportation conditions. The viability and proliferation of cancer cells were assessed using trypan blue staining and quantitative CCK-8 kit, respectively. The developed beads allowed cancer cells survival up to 4 weeks with less frequent maintenance measures such as change of the culture media or subculture of cells. In addition, the recovery of cancer cells and proliferation pattern were significantly faster with better outcomes in the developed 3D alginate beads compared to the standard cryopreservation of cells or the 2D culture conditions. The 3D alginate beads also supported the viability of cells while the shipment at room temperature for a duration of up to 5 days with no humidity or CO2 support. Therefore, 3D culture in alginate beads can be used to store or ship biological cells with ease at room temperature with minimal preparations.
format Article
author Alallam, Batoul
Oo, May Kyaw
Ibrahim, Wisam Nabeel
Doolaanea, Abd Almonem
author_facet Alallam, Batoul
Oo, May Kyaw
Ibrahim, Wisam Nabeel
Doolaanea, Abd Almonem
author_sort Alallam, Batoul
title 3D culture of cancer cells in alginate hydrogel beads as an effective technique for emergency cell storage and transportation in the pandemic era
title_short 3D culture of cancer cells in alginate hydrogel beads as an effective technique for emergency cell storage and transportation in the pandemic era
title_full 3D culture of cancer cells in alginate hydrogel beads as an effective technique for emergency cell storage and transportation in the pandemic era
title_fullStr 3D culture of cancer cells in alginate hydrogel beads as an effective technique for emergency cell storage and transportation in the pandemic era
title_full_unstemmed 3D culture of cancer cells in alginate hydrogel beads as an effective technique for emergency cell storage and transportation in the pandemic era
title_sort 3d culture of cancer cells in alginate hydrogel beads as an effective technique for emergency cell storage and transportation in the pandemic era
publisher Wiley
publishDate 2021
url http://irep.iium.edu.my/95076/13/95076_3D%20culture%20of%20cancer%20cells%20in%20alginate%20hydrogel%20beads%20as.pdf
http://irep.iium.edu.my/95076/
http://wileyonlinelibrary.com/journal/jcmm
https://doi.org/10.1111/jcmm.17078
_version_ 1720436672499286016
score 13.211869

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