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Extraction and quantification of eugenol from clove buds using HPLC

Background: Eugenol is the main constituent of clove essential oil. Past studies have found that clove oil has diverse uses in the pharmaceutical field due to its antioxidant, antibacterial and anesthetic properties. Objective: This work compares the performance of different extraction methods and...

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Main Authors:	Shafira, Khadiza Fitri, Azad, Abul Kalam, Labu, Zubair Khalid, Helal Uddin, A.B.M.
Format:	Article
Language:	English
Published:	Bentham Science 2020
Subjects:	QD Chemistry RM214 Diet therapy. Diet and dietectics in disease RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:	http://irep.iium.edu.my/93611/2/93611_Extraction%20and%20quantification%20of%20eugenol.pdf http://irep.iium.edu.my/93611/ https://www.eurekaselect.com/185015/article https://doi.org/10.2174/2213240607999200818161356
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spelling	my.iium.irep.936112022-11-07T02:20:20Z http://irep.iium.edu.my/93611/ Extraction and quantification of eugenol from clove buds using HPLC Shafira, Khadiza Fitri Azad, Abul Kalam Labu, Zubair Khalid Helal Uddin, A.B.M. QD Chemistry RM214 Diet therapy. Diet and dietectics in disease RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Background: Eugenol is the main constituent of clove essential oil. Past studies have found that clove oil has diverse uses in the pharmaceutical field due to its antioxidant, antibacterial and anesthetic properties. Objective: This work compares the performance of different extraction methods and factors and identifies the effect of the treatments on oil yields and eugenol content. Materials and Methods: Maceration, Hydro distillation, microwave-assisted extraction (MAE),and Soxhlet were performed. The best technique was identified according to yield and content. Further studies were conducted to examine the effects of different factors, such as solvent types (ethanol and methanol) and sample-to-solvent ratio (1:10 and 1:15). HPLC UV-Vis was utilized in the analysis of eugenol concentration. Results and Discussion: Soxhlet extraction provided the highest yield (39.98%) and eugenol con- tent (15.83%), compared to other methods. The results observed from several Soxhlet extraction factors showed that there is no significant difference between the different factors. In the mean- time, methanol 1:15 provided the greatest amount of yields (57.83%) and eugenol content (22.21%). In this regard, the higher ratio resulted in higher eugenol content. Conclusion: The results obtained are less comparable because the processing time, the working sol- vent, and the separation technique were carried out differently for each method. In the meantime, as there is no past study that compared the selected methods and factors, this study’s findings will contribute substantially to fill the gap in this field. Bentham Science 2020-08-18 Article PeerReviewed application/pdf en http://irep.iium.edu.my/93611/2/93611_Extraction%20and%20quantification%20of%20eugenol.pdf Shafira, Khadiza Fitri and Azad, Abul Kalam and Labu, Zubair Khalid and Helal Uddin, A.B.M. (2020) Extraction and quantification of eugenol from clove buds using HPLC. Current Chromatography, 7 (1). pp. 17-23. ISSN 2213-2406 E-ISSN 2213-2414 https://www.eurekaselect.com/185015/article https://doi.org/10.2174/2213240607999200818161356
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	QD Chemistry RM214 Diet therapy. Diet and dietectics in disease RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle	QD Chemistry RM214 Diet therapy. Diet and dietectics in disease RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Shafira, Khadiza Fitri Azad, Abul Kalam Labu, Zubair Khalid Helal Uddin, A.B.M. Extraction and quantification of eugenol from clove buds using HPLC
description	Background: Eugenol is the main constituent of clove essential oil. Past studies have found that clove oil has diverse uses in the pharmaceutical field due to its antioxidant, antibacterial and anesthetic properties. Objective: This work compares the performance of different extraction methods and factors and identifies the effect of the treatments on oil yields and eugenol content. Materials and Methods: Maceration, Hydro distillation, microwave-assisted extraction (MAE),and Soxhlet were performed. The best technique was identified according to yield and content. Further studies were conducted to examine the effects of different factors, such as solvent types (ethanol and methanol) and sample-to-solvent ratio (1:10 and 1:15). HPLC UV-Vis was utilized in the analysis of eugenol concentration. Results and Discussion: Soxhlet extraction provided the highest yield (39.98%) and eugenol con- tent (15.83%), compared to other methods. The results observed from several Soxhlet extraction factors showed that there is no significant difference between the different factors. In the mean- time, methanol 1:15 provided the greatest amount of yields (57.83%) and eugenol content (22.21%). In this regard, the higher ratio resulted in higher eugenol content. Conclusion: The results obtained are less comparable because the processing time, the working sol- vent, and the separation technique were carried out differently for each method. In the meantime, as there is no past study that compared the selected methods and factors, this study’s findings will contribute substantially to fill the gap in this field.
format	Article
author	Shafira, Khadiza Fitri Azad, Abul Kalam Labu, Zubair Khalid Helal Uddin, A.B.M.
author_facet	Shafira, Khadiza Fitri Azad, Abul Kalam Labu, Zubair Khalid Helal Uddin, A.B.M.
author_sort	Shafira, Khadiza Fitri
title	Extraction and quantification of eugenol from clove buds using HPLC
title_short	Extraction and quantification of eugenol from clove buds using HPLC
title_full	Extraction and quantification of eugenol from clove buds using HPLC
title_fullStr	Extraction and quantification of eugenol from clove buds using HPLC
title_full_unstemmed	Extraction and quantification of eugenol from clove buds using HPLC
title_sort	extraction and quantification of eugenol from clove buds using hplc
publisher	Bentham Science
publishDate	2020
url	http://irep.iium.edu.my/93611/2/93611_Extraction%20and%20quantification%20of%20eugenol.pdf http://irep.iium.edu.my/93611/ https://www.eurekaselect.com/185015/article https://doi.org/10.2174/2213240607999200818161356
_version_	1751535903935823872
score	13.222552

Extraction and quantification of eugenol from clove buds using HPLC

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