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In Vitro Cytotoxicity Analysis of Bioceramic Root Canal Sealers on Human Gingival Fibroblast Cells and Human Osteoblast-like Cells

Introduction: Cytotoxicity of bioceramic (BC) root canal sealers (RCS) are not well understood due to the limited scientific data. Previous research focused on comparing the conventional and BC RCS but there was no comparison between the latter. This study aims to evaluate the cytotoxicity of BC RCS...

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Main Authors: Ja'apar, Siti Aisyah Nadirah, Ichwan, Solachuddin J. A., Mustaffa, Musliana
Format: Conference or Workshop Item
Language:English
English
English
English
Published: 2021
Subjects:
L Education (General)
RK Dentistry
Online Access:http://irep.iium.edu.my/93136/1/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_slide.pdf
http://irep.iium.edu.my/93136/2/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_schedule.pdf
http://irep.iium.edu.my/93136/3/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_abstract.docx
http://irep.iium.edu.my/93136/4/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_cert.pdf
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spelling my.iium.irep.931362021-11-16T07:23:35Z http://irep.iium.edu.my/93136/ In Vitro Cytotoxicity Analysis of Bioceramic Root Canal Sealers on Human Gingival Fibroblast Cells and Human Osteoblast-like Cells Ja'apar, Siti Aisyah Nadirah Ichwan, Solachuddin J. A. Mustaffa, Musliana L Education (General) RK Dentistry Introduction: Cytotoxicity of bioceramic (BC) root canal sealers (RCS) are not well understood due to the limited scientific data. Previous research focused on comparing the conventional and BC RCS but there was no comparison between the latter. This study aims to evaluate the cytotoxicity of BC RCS and to propose the material that is less cytotoxic for clinical purposes. Methods: GuttaFlow Bioseal, MTA Fillapex, CeraSeal BC RCS and iRoot SP RCS were mixed according to the manufacturers’ instructions and placed into a sterilised cylindrical silicone mold of 5mm diameter and 3mm thickness. Fourier transform infrared spectroscopy was used to analyse changes in the quality and consistency of all BC RCS. Human gingival fibroblast (hGFC) and osteoblast-like cells (hOLC) were cultivated and exposed to the material extracts for 24 h, 48 h and 72 h in a 5% CO2 humidified incubator at 37 °C. The MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay was conducted to determine cell viability. Cell viability was qualitatively graded as severe (< 30%), moderate (30% - 60%), mild (60% - 90%), or non-cytotoxic (> 90%). Results: GuttaFlow Bioseal showed less cytotoxicity followed by CeraSeal BC RCS, iRoot SP RCS, and MTA Fillapex when exposed to hGFC. The percentage of cell viability for all BC RCS was increased from 24 h to 72 h. When the hOLC were exposed to material extracts, GuttaFlow Bioseal and MTA Fillapex showed inconsistent cytotoxic effects, however GuttaFlow Bioseal appeared to be less cytotoxic than MTA Fillapex. CeraSeal BC RCS and iRoot SP RCS were more cytotoxic throughout all incubation periods. Conclusions: It could be suggested that the cytotoxicity of BC RCS was influenced by the types of materials, incubation periods and types of culture cells. Clinicians should select BC RCS that have low cytotoxic effects during root canal treatment procedure. 2021-10-16 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/93136/1/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_slide.pdf application/pdf en http://irep.iium.edu.my/93136/2/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_schedule.pdf application/pdf en http://irep.iium.edu.my/93136/3/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_abstract.docx application/pdf en http://irep.iium.edu.my/93136/4/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_cert.pdf Ja'apar, Siti Aisyah Nadirah and Ichwan, Solachuddin J. A. and Mustaffa, Musliana (2021) In Vitro Cytotoxicity Analysis of Bioceramic Root Canal Sealers on Human Gingival Fibroblast Cells and Human Osteoblast-like Cells. In: International Dental Students Conference 2021, Online Conference. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
English
topic L Education (General)
RK Dentistry
spellingShingle L Education (General)
RK Dentistry
Ja'apar, Siti Aisyah Nadirah
Ichwan, Solachuddin J. A.
Mustaffa, Musliana
In Vitro Cytotoxicity Analysis of Bioceramic Root Canal Sealers on Human Gingival Fibroblast Cells and Human Osteoblast-like Cells
description Introduction: Cytotoxicity of bioceramic (BC) root canal sealers (RCS) are not well understood due to the limited scientific data. Previous research focused on comparing the conventional and BC RCS but there was no comparison between the latter. This study aims to evaluate the cytotoxicity of BC RCS and to propose the material that is less cytotoxic for clinical purposes. Methods: GuttaFlow Bioseal, MTA Fillapex, CeraSeal BC RCS and iRoot SP RCS were mixed according to the manufacturers’ instructions and placed into a sterilised cylindrical silicone mold of 5mm diameter and 3mm thickness. Fourier transform infrared spectroscopy was used to analyse changes in the quality and consistency of all BC RCS. Human gingival fibroblast (hGFC) and osteoblast-like cells (hOLC) were cultivated and exposed to the material extracts for 24 h, 48 h and 72 h in a 5% CO2 humidified incubator at 37 °C. The MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay was conducted to determine cell viability. Cell viability was qualitatively graded as severe (< 30%), moderate (30% - 60%), mild (60% - 90%), or non-cytotoxic (> 90%). Results: GuttaFlow Bioseal showed less cytotoxicity followed by CeraSeal BC RCS, iRoot SP RCS, and MTA Fillapex when exposed to hGFC. The percentage of cell viability for all BC RCS was increased from 24 h to 72 h. When the hOLC were exposed to material extracts, GuttaFlow Bioseal and MTA Fillapex showed inconsistent cytotoxic effects, however GuttaFlow Bioseal appeared to be less cytotoxic than MTA Fillapex. CeraSeal BC RCS and iRoot SP RCS were more cytotoxic throughout all incubation periods. Conclusions: It could be suggested that the cytotoxicity of BC RCS was influenced by the types of materials, incubation periods and types of culture cells. Clinicians should select BC RCS that have low cytotoxic effects during root canal treatment procedure.
format Conference or Workshop Item
author Ja'apar, Siti Aisyah Nadirah
Ichwan, Solachuddin J. A.
Mustaffa, Musliana
author_facet Ja'apar, Siti Aisyah Nadirah
Ichwan, Solachuddin J. A.
Mustaffa, Musliana
author_sort Ja'apar, Siti Aisyah Nadirah
title In Vitro Cytotoxicity Analysis of Bioceramic Root Canal Sealers on Human Gingival Fibroblast Cells and Human Osteoblast-like Cells
title_short In Vitro Cytotoxicity Analysis of Bioceramic Root Canal Sealers on Human Gingival Fibroblast Cells and Human Osteoblast-like Cells
title_full In Vitro Cytotoxicity Analysis of Bioceramic Root Canal Sealers on Human Gingival Fibroblast Cells and Human Osteoblast-like Cells
title_fullStr In Vitro Cytotoxicity Analysis of Bioceramic Root Canal Sealers on Human Gingival Fibroblast Cells and Human Osteoblast-like Cells
title_full_unstemmed In Vitro Cytotoxicity Analysis of Bioceramic Root Canal Sealers on Human Gingival Fibroblast Cells and Human Osteoblast-like Cells
title_sort in vitro cytotoxicity analysis of bioceramic root canal sealers on human gingival fibroblast cells and human osteoblast-like cells
publishDate 2021
url http://irep.iium.edu.my/93136/1/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_slide.pdf
http://irep.iium.edu.my/93136/2/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_schedule.pdf
http://irep.iium.edu.my/93136/3/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_abstract.docx
http://irep.iium.edu.my/93136/4/93136_In%20Vitro%20Cytotoxicity%20Analysis%20of%20Bioceramic%20Root%20Canal%20Sealers_cert.pdf
http://irep.iium.edu.my/93136/
_version_ 1717093001383116800
score 13.211869

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