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Optimisation of Culture Condition for Sacha Inchi (Plukenetia Volubilis) Callus Induction

Plukenetia volubilis or commonly known as sacha inchi is reported to produce wide range of health‐promoting bioactive metabolites. These metabolites functions as supplements in eradicating various types of diseases. Sacha inchi has large edible seeds that are rich in phenolic content, minerals and e...

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Main Authors: Rosle, Norsyahira, Mohd Jalil, Jasrina, Abdul Wahab, Widya, Yunus, Muhamad Fahmi, Meenakshi Sundram, Tamil Chelvan
Format: Article
Language:English
Published: Zibeline International Publishing Sdn. Bhd. 2021
Subjects:
QH301 Biology
Online Access:http://irep.iium.edu.my/92677/1/92677_Optimisation%20of%20Culture%20Condition.pdf
http://irep.iium.edu.my/92677/
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spelling my.iium.irep.926772021-10-04T04:35:28Z http://irep.iium.edu.my/92677/ Optimisation of Culture Condition for Sacha Inchi (Plukenetia Volubilis) Callus Induction Rosle, Norsyahira Mohd Jalil, Jasrina Abdul Wahab, Widya Yunus, Muhamad Fahmi Meenakshi Sundram, Tamil Chelvan QH301 Biology Plukenetia volubilis or commonly known as sacha inchi is reported to produce wide range of health‐promoting bioactive metabolites. These metabolites functions as supplements in eradicating various types of diseases. Sacha inchi has large edible seeds that are rich in phenolic content, minerals and essential fatty acid, such as omega 3 (ω-3), omega 6 (ω-6), omega 7 (ω-7), and omega 9 (ω-9). In vitro cultures could serve as alternative in producing many essential sacha inchi bioactive compounds. As an initial step towards initiating in vitro culture, the effect of 2,4-D and TDZ on inducing callus cultures were investigated. Two different explants, sacha inchi leaf and male flower were used in this study. Surface sterilization of sacha inchi was first optimized to overcome culture contamination. The most effective surface sterilization is by using 70% ethanol (30 seconds) and 0.5% sodium hypochlorite (8 minutes), which resulted in 82.5% and 95% survival rate for leaf and flower explants respectively. Next, for calli induction the explants were cultured on MS medium supplemented with different concentrations of 2,4-D and TDZ, either alone or in combination and grown at 24 hours dark photoperiods. The morphology and size of callus were observed. The results obtained from the experiment varied depending on the treatments, producing either friable or compact calli of creamy white, pure white or brownish colour. For both, leaf and male flower explants, MS medium supplemented with 3% (w/v) sucrose in combination with 1.0 mg/L 2,4-D and 0.005 mg/L TDZ recorded the best response in term of callus size, forming friable creamy white callus. Zibeline International Publishing Sdn. Bhd. 2021-09-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/92677/1/92677_Optimisation%20of%20Culture%20Condition.pdf Rosle, Norsyahira and Mohd Jalil, Jasrina and Abdul Wahab, Widya and Yunus, Muhamad Fahmi and Meenakshi Sundram, Tamil Chelvan (2021) Optimisation of Culture Condition for Sacha Inchi (Plukenetia Volubilis) Callus Induction. Tropical Agroecosystems, 2 (2). pp. 87-90. E-ISSN 2735-0274 10.26480/taec.02.2021.87.90
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Rosle, Norsyahira
Mohd Jalil, Jasrina
Abdul Wahab, Widya
Yunus, Muhamad Fahmi
Meenakshi Sundram, Tamil Chelvan
Optimisation of Culture Condition for Sacha Inchi (Plukenetia Volubilis) Callus Induction
description Plukenetia volubilis or commonly known as sacha inchi is reported to produce wide range of health‐promoting bioactive metabolites. These metabolites functions as supplements in eradicating various types of diseases. Sacha inchi has large edible seeds that are rich in phenolic content, minerals and essential fatty acid, such as omega 3 (ω-3), omega 6 (ω-6), omega 7 (ω-7), and omega 9 (ω-9). In vitro cultures could serve as alternative in producing many essential sacha inchi bioactive compounds. As an initial step towards initiating in vitro culture, the effect of 2,4-D and TDZ on inducing callus cultures were investigated. Two different explants, sacha inchi leaf and male flower were used in this study. Surface sterilization of sacha inchi was first optimized to overcome culture contamination. The most effective surface sterilization is by using 70% ethanol (30 seconds) and 0.5% sodium hypochlorite (8 minutes), which resulted in 82.5% and 95% survival rate for leaf and flower explants respectively. Next, for calli induction the explants were cultured on MS medium supplemented with different concentrations of 2,4-D and TDZ, either alone or in combination and grown at 24 hours dark photoperiods. The morphology and size of callus were observed. The results obtained from the experiment varied depending on the treatments, producing either friable or compact calli of creamy white, pure white or brownish colour. For both, leaf and male flower explants, MS medium supplemented with 3% (w/v) sucrose in combination with 1.0 mg/L 2,4-D and 0.005 mg/L TDZ recorded the best response in term of callus size, forming friable creamy white callus.
format Article
author Rosle, Norsyahira
Mohd Jalil, Jasrina
Abdul Wahab, Widya
Yunus, Muhamad Fahmi
Meenakshi Sundram, Tamil Chelvan
author_facet Rosle, Norsyahira
Mohd Jalil, Jasrina
Abdul Wahab, Widya
Yunus, Muhamad Fahmi
Meenakshi Sundram, Tamil Chelvan
author_sort Rosle, Norsyahira
title Optimisation of Culture Condition for Sacha Inchi (Plukenetia Volubilis) Callus Induction
title_short Optimisation of Culture Condition for Sacha Inchi (Plukenetia Volubilis) Callus Induction
title_full Optimisation of Culture Condition for Sacha Inchi (Plukenetia Volubilis) Callus Induction
title_fullStr Optimisation of Culture Condition for Sacha Inchi (Plukenetia Volubilis) Callus Induction
title_full_unstemmed Optimisation of Culture Condition for Sacha Inchi (Plukenetia Volubilis) Callus Induction
title_sort optimisation of culture condition for sacha inchi (plukenetia volubilis) callus induction
publisher Zibeline International Publishing Sdn. Bhd.
publishDate 2021
url http://irep.iium.edu.my/92677/1/92677_Optimisation%20of%20Culture%20Condition.pdf
http://irep.iium.edu.my/92677/
_version_ 1713199558266191872
score 13.211869

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