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The supervirulence plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation efficiency of Hevea brasiliensis

Problem statement: The present study investigates the ability of the pTok47 supervirulence plasmid form Agrobacterium tumefaciens A281 to enhance genetic transformation in Hevea brasiliensis. Approach: Hevea anther callus was transformed via Agrobacterium-mediated genetic transformation using tw...

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Main Authors: Pappusamy, Arokiaraj, R, Leelawathy, Hoong, Yeet Yeang
Format: Article
Language:English
Published: Science Publications 2009
Subjects:
QR Microbiology
Online Access:http://irep.iium.edu.my/916/1/The_Supervirulence_Plasmid_pToK47_from_Agrobacterium_tumefaciens_A281.pdf
http://irep.iium.edu.my/916/
http://thescipub.com/abstract/10.3844/ajbbsp.2009.137.141
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spelling my.iium.irep.9162011-07-12T08:21:34Z http://irep.iium.edu.my/916/ The supervirulence plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation efficiency of Hevea brasiliensis Pappusamy, Arokiaraj R, Leelawathy Hoong, Yeet Yeang QR Microbiology Problem statement: The present study investigates the ability of the pTok47 supervirulence plasmid form Agrobacterium tumefaciens A281 to enhance genetic transformation in Hevea brasiliensis. Approach: Hevea anther callus was transformed via Agrobacterium-mediated genetic transformation using two strains of Agrobacterium (GV2260 and GV3850) harboring the human serum albumin cDNA and the supervirulent plasmid pToK47 from Agrobacterium tumefaciens A281. The transformed callus was selected using kanamycin as the selection agent Results: The Agrobacterium strain GV2260 benefited from the presence of the supervirulence plasmid in giving a higher frequency of 7.4% transformed callus, 344.8% embryogenesis and 11.6% plantlet production compared to the corresponding strain on its own giving 0.9% transformed callus, 204.5% embryogenesis and 4.4% plantlet production. Similarly, Agrobacterium strain GV3850 conferred a higher frequency using the supervirulent plasmid, resulting in 3.5% transformed callus, 138.5% embryogenesis and 3.5% plantlet production compared to the corresponding strain on its own giving 0.7% transformed callus, 137.5% embryogenesis and 9.0% plantlet production. These findings were confirmed by the Wilcoxon Signed Rank Test that compared the effectiveness of the supervirulence plasmid in increasing the rate of genetic transformation in the calli surviving in kanamycin growth medium for GV2260 (p<0.001) and for GV3850 (p<0.05). Conclusion: This study showed that both Agrobacterium strains benefited from the presence of the supervirulence plasmid in giving a higher frequency of transformed callus, embryoids and plantlets. These results suggest that a highly virulent binary vector system might prove especially useful in generating high frequency transformation of Hevea. Science Publications 2009 Article REM application/pdf en http://irep.iium.edu.my/916/1/The_Supervirulence_Plasmid_pToK47_from_Agrobacterium_tumefaciens_A281.pdf Pappusamy, Arokiaraj and R, Leelawathy and Hoong, Yeet Yeang (2009) The supervirulence plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation efficiency of Hevea brasiliensis. American Journal of Biochemistry and Biotechnology, 5 (3). pp. 137-141. ISSN 15533468 http://thescipub.com/abstract/10.3844/ajbbsp.2009.137.141 doi:10.3844/ajbbsp.2009.137.141
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Pappusamy, Arokiaraj
R, Leelawathy
Hoong, Yeet Yeang
The supervirulence plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation efficiency of Hevea brasiliensis
description Problem statement: The present study investigates the ability of the pTok47 supervirulence plasmid form Agrobacterium tumefaciens A281 to enhance genetic transformation in Hevea brasiliensis. Approach: Hevea anther callus was transformed via Agrobacterium-mediated genetic transformation using two strains of Agrobacterium (GV2260 and GV3850) harboring the human serum albumin cDNA and the supervirulent plasmid pToK47 from Agrobacterium tumefaciens A281. The transformed callus was selected using kanamycin as the selection agent Results: The Agrobacterium strain GV2260 benefited from the presence of the supervirulence plasmid in giving a higher frequency of 7.4% transformed callus, 344.8% embryogenesis and 11.6% plantlet production compared to the corresponding strain on its own giving 0.9% transformed callus, 204.5% embryogenesis and 4.4% plantlet production. Similarly, Agrobacterium strain GV3850 conferred a higher frequency using the supervirulent plasmid, resulting in 3.5% transformed callus, 138.5% embryogenesis and 3.5% plantlet production compared to the corresponding strain on its own giving 0.7% transformed callus, 137.5% embryogenesis and 9.0% plantlet production. These findings were confirmed by the Wilcoxon Signed Rank Test that compared the effectiveness of the supervirulence plasmid in increasing the rate of genetic transformation in the calli surviving in kanamycin growth medium for GV2260 (p<0.001) and for GV3850 (p<0.05). Conclusion: This study showed that both Agrobacterium strains benefited from the presence of the supervirulence plasmid in giving a higher frequency of transformed callus, embryoids and plantlets. These results suggest that a highly virulent binary vector system might prove especially useful in generating high frequency transformation of Hevea.
format Article
author Pappusamy, Arokiaraj
R, Leelawathy
Hoong, Yeet Yeang
author_facet Pappusamy, Arokiaraj
R, Leelawathy
Hoong, Yeet Yeang
author_sort Pappusamy, Arokiaraj
title The supervirulence plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation efficiency of Hevea brasiliensis
title_short The supervirulence plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation efficiency of Hevea brasiliensis
title_full The supervirulence plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation efficiency of Hevea brasiliensis
title_fullStr The supervirulence plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation efficiency of Hevea brasiliensis
title_full_unstemmed The supervirulence plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation efficiency of Hevea brasiliensis
title_sort supervirulence plasmid ptok47 from agrobacterium tumefaciens a281 improves transformation efficiency of hevea brasiliensis
publisher Science Publications
publishDate 2009
url http://irep.iium.edu.my/916/1/The_Supervirulence_Plasmid_pToK47_from_Agrobacterium_tumefaciens_A281.pdf
http://irep.iium.edu.my/916/
http://thescipub.com/abstract/10.3844/ajbbsp.2009.137.141
_version_ 1643604692892123136
score 13.211869

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