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Effect of pH on hematoxylin and eosin staining of rats intestinal tissue

Background: Optimising hematoxylin and eosin (H&E) protocol does not only ensure reproducible and excellent staining quality, it also helps to optimise the use of lab materials and resources. This study investigates the effect of hematoxylin pH on H&E staining quality. Method: In an...

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Main Authors: Mohd Radzuan, Hazulin, Wan Omar, Wan Fatein Nabeila, Halim, Mohd Dhiyaulhaq
Format: Conference or Workshop Item
Language:English
English
Published: 2020
Subjects:
Q Science (General)
QD Chemistry
R Medicine (General)
RB Pathology
RZ Other systems of medicine
Online Access:http://irep.iium.edu.my/90853/2/poster%20KRD2020.pdf
http://irep.iium.edu.my/90853/3/HAZULIN%20MOHD%20RADZUAN%2013.pdf
http://irep.iium.edu.my/90853/
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spelling my.iium.irep.908532021-11-24T00:01:29Z http://irep.iium.edu.my/90853/ Effect of pH on hematoxylin and eosin staining of rats intestinal tissue Mohd Radzuan, Hazulin Wan Omar, Wan Fatein Nabeila Halim, Mohd Dhiyaulhaq Q Science (General) QD Chemistry R Medicine (General) RB Pathology RZ Other systems of medicine Background: Optimising hematoxylin and eosin (H&E) protocol does not only ensure reproducible and excellent staining quality, it also helps to optimise the use of lab materials and resources. This study investigates the effect of hematoxylin pH on H&E staining quality. Method: In an experimental study, 3 μm tissue sections of formalin-fixed paraffin embedded intestinal tissues of three healthy female Sprague-Dawley rats were subjected to the standard H&E protocol. Modified Gill’s hematoxylins of four pH (2.5, 2.75, 3.0 and 3.5) and eosin (pH 5.0) as counterstain were used. Results: At hematoxypin pH 2.5, the tissues appeared acidophilic with indistinctive epithelial lining. At pH 2.75, the hematoxylin dye was most prominent with good balance of staining, and crisp epithelial lining was observed. At pH 3.0 or higher, although the lining was crisp, blue-staining mucin were observed. Conclusion: pH 2.74 is the most optimal pH to achieve balance of coloration and definitive epithelial lining in H&E staining. 2020-10-01 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/90853/2/poster%20KRD2020.pdf application/pdf en http://irep.iium.edu.my/90853/3/HAZULIN%20MOHD%20RADZUAN%2013.pdf Mohd Radzuan, Hazulin and Wan Omar, Wan Fatein Nabeila and Halim, Mohd Dhiyaulhaq (2020) Effect of pH on hematoxylin and eosin staining of rats intestinal tissue. In: Kuantan Research Day 2020: A National Online Event for E-poster Presentation, 1st October 2020 – 5th January 2021, Kuantan, Pahang. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic Q Science (General)
QD Chemistry
R Medicine (General)
RB Pathology
RZ Other systems of medicine
spellingShingle Q Science (General)
QD Chemistry
R Medicine (General)
RB Pathology
RZ Other systems of medicine
Mohd Radzuan, Hazulin
Wan Omar, Wan Fatein Nabeila
Halim, Mohd Dhiyaulhaq
Effect of pH on hematoxylin and eosin staining of rats intestinal tissue
description Background: Optimising hematoxylin and eosin (H&E) protocol does not only ensure reproducible and excellent staining quality, it also helps to optimise the use of lab materials and resources. This study investigates the effect of hematoxylin pH on H&E staining quality. Method: In an experimental study, 3 μm tissue sections of formalin-fixed paraffin embedded intestinal tissues of three healthy female Sprague-Dawley rats were subjected to the standard H&E protocol. Modified Gill’s hematoxylins of four pH (2.5, 2.75, 3.0 and 3.5) and eosin (pH 5.0) as counterstain were used. Results: At hematoxypin pH 2.5, the tissues appeared acidophilic with indistinctive epithelial lining. At pH 2.75, the hematoxylin dye was most prominent with good balance of staining, and crisp epithelial lining was observed. At pH 3.0 or higher, although the lining was crisp, blue-staining mucin were observed. Conclusion: pH 2.74 is the most optimal pH to achieve balance of coloration and definitive epithelial lining in H&E staining.
format Conference or Workshop Item
author Mohd Radzuan, Hazulin
Wan Omar, Wan Fatein Nabeila
Halim, Mohd Dhiyaulhaq
author_facet Mohd Radzuan, Hazulin
Wan Omar, Wan Fatein Nabeila
Halim, Mohd Dhiyaulhaq
author_sort Mohd Radzuan, Hazulin
title Effect of pH on hematoxylin and eosin staining of rats intestinal tissue
title_short Effect of pH on hematoxylin and eosin staining of rats intestinal tissue
title_full Effect of pH on hematoxylin and eosin staining of rats intestinal tissue
title_fullStr Effect of pH on hematoxylin and eosin staining of rats intestinal tissue
title_full_unstemmed Effect of pH on hematoxylin and eosin staining of rats intestinal tissue
title_sort effect of ph on hematoxylin and eosin staining of rats intestinal tissue
publishDate 2020
url http://irep.iium.edu.my/90853/2/poster%20KRD2020.pdf
http://irep.iium.edu.my/90853/3/HAZULIN%20MOHD%20RADZUAN%2013.pdf
http://irep.iium.edu.my/90853/
_version_ 1718925597612179456
score 13.211869

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