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A method review of surgically induced hypertension in pregnant rats model: modified Reduced Uterine Perfusion Pressure (RUPP)

Background: There are various animal models used in the study of pregnancy-associated hypertensive disorders. RUPP is one of the methods used to induce hypertension via surgical procedure by clipping both ovarian and uterine arteries with specified internal diameter of silver clip. In this study, we...

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Main Authors: Zulkiflee, Khodijah, Mohd Zainudin, Maizura, Zainal Abidin, Fadhilah, Ismawi, Hidayatul Radziah, Yusoff Azmi Merican, Yusoff Sharizal
Format: Conference or Workshop Item
Language:English
Published: Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2021
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/90828/1/90828_A%20method%20review%20of%20surgically.pdf
http://irep.iium.edu.my/90828/
https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/598/564
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spelling my.iium.irep.908282021-08-06T12:45:26Z http://irep.iium.edu.my/90828/ A method review of surgically induced hypertension in pregnant rats model: modified Reduced Uterine Perfusion Pressure (RUPP) Zulkiflee, Khodijah Mohd Zainudin, Maizura Zainal Abidin, Fadhilah Ismawi, Hidayatul Radziah Yusoff Azmi Merican, Yusoff Sharizal R Medicine (General) Background: There are various animal models used in the study of pregnancy-associated hypertensive disorders. RUPP is one of the methods used to induce hypertension via surgical procedure by clipping both ovarian and uterine arteries with specified internal diameter of silver clip. In this study, we modified the method by using a surgical suture instead of clip. Purpose: The objective of this study is to establish hypertension in a pregnant rat model by using a modified method of RUPP. Methodology: Three female Sprague-Dawley rats were used. One of them was non-pregnant rat (C) and the other two rats (R1 & R2) were mated. The establishment of pregnancy were confirmed with the visualization of sperm and it was recorded as D0 of gestation. On D14 of gestation, both uterine and ovarian arteries were tied in R1 by using a suture while in R2, only uterine arteries were tied. Blood pressure was measured via tail-cuff method at D20 of gestation. Result: Complete fetal resorption occurred in R1 and R2 died post-operatively due to infection. Conclusion: This method caused high morbidity and mortality and we could not establish hypertension in pregnant animal model. The alternative methods for induction of hypertension in pregnant animal model are by using a drug that inhibit Nitric Oxide synthase (L-NAME) or by genetic manipulation. Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2021-04-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/90828/1/90828_A%20method%20review%20of%20surgically.pdf Zulkiflee, Khodijah and Mohd Zainudin, Maizura and Zainal Abidin, Fadhilah and Ismawi, Hidayatul Radziah and Yusoff Azmi Merican, Yusoff Sharizal (2021) A method review of surgically induced hypertension in pregnant rats model: modified Reduced Uterine Perfusion Pressure (RUPP). In: 4th Kuantan Research Day 2020, Virtual. https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/598/564
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Zulkiflee, Khodijah
Mohd Zainudin, Maizura
Zainal Abidin, Fadhilah
Ismawi, Hidayatul Radziah
Yusoff Azmi Merican, Yusoff Sharizal
A method review of surgically induced hypertension in pregnant rats model: modified Reduced Uterine Perfusion Pressure (RUPP)
description Background: There are various animal models used in the study of pregnancy-associated hypertensive disorders. RUPP is one of the methods used to induce hypertension via surgical procedure by clipping both ovarian and uterine arteries with specified internal diameter of silver clip. In this study, we modified the method by using a surgical suture instead of clip. Purpose: The objective of this study is to establish hypertension in a pregnant rat model by using a modified method of RUPP. Methodology: Three female Sprague-Dawley rats were used. One of them was non-pregnant rat (C) and the other two rats (R1 & R2) were mated. The establishment of pregnancy were confirmed with the visualization of sperm and it was recorded as D0 of gestation. On D14 of gestation, both uterine and ovarian arteries were tied in R1 by using a suture while in R2, only uterine arteries were tied. Blood pressure was measured via tail-cuff method at D20 of gestation. Result: Complete fetal resorption occurred in R1 and R2 died post-operatively due to infection. Conclusion: This method caused high morbidity and mortality and we could not establish hypertension in pregnant animal model. The alternative methods for induction of hypertension in pregnant animal model are by using a drug that inhibit Nitric Oxide synthase (L-NAME) or by genetic manipulation.
format Conference or Workshop Item
author Zulkiflee, Khodijah
Mohd Zainudin, Maizura
Zainal Abidin, Fadhilah
Ismawi, Hidayatul Radziah
Yusoff Azmi Merican, Yusoff Sharizal
author_facet Zulkiflee, Khodijah
Mohd Zainudin, Maizura
Zainal Abidin, Fadhilah
Ismawi, Hidayatul Radziah
Yusoff Azmi Merican, Yusoff Sharizal
author_sort Zulkiflee, Khodijah
title A method review of surgically induced hypertension in pregnant rats model: modified Reduced Uterine Perfusion Pressure (RUPP)
title_short A method review of surgically induced hypertension in pregnant rats model: modified Reduced Uterine Perfusion Pressure (RUPP)
title_full A method review of surgically induced hypertension in pregnant rats model: modified Reduced Uterine Perfusion Pressure (RUPP)
title_fullStr A method review of surgically induced hypertension in pregnant rats model: modified Reduced Uterine Perfusion Pressure (RUPP)
title_full_unstemmed A method review of surgically induced hypertension in pregnant rats model: modified Reduced Uterine Perfusion Pressure (RUPP)
title_sort method review of surgically induced hypertension in pregnant rats model: modified reduced uterine perfusion pressure (rupp)
publisher Kulliyyah of Allied Health Sciences, International Islamic University Malaysia
publishDate 2021
url http://irep.iium.edu.my/90828/1/90828_A%20method%20review%20of%20surgically.pdf
http://irep.iium.edu.my/90828/
https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/598/564
_version_ 1707765669693489152
score 13.211869

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