Cytotoxic activity of eurycoma longifolia jack root extract against nasopharyngeal carcinoma cell line
Background: Eurycoma longifolia (E. longifolia) or Tongkat Ali is a tree that grows in southeast Asia, the roots of which contain bioactive components that exhibit cytotoxic properties against various cancer cell lines. However, no study has been conducted to relate the cytotoxic properties agains...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Pharmacognosy Network Worldwide
2021
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/90816/7/90816_%20Cytotoxic%20activity%20of%20eurycoma%20longifolia%20jack%20root%20extract.pdf http://irep.iium.edu.my/90816/13/90816_Cytotoxic%20activity%20of%20eurycoma%20longifolia%20jack%20root_SCOPUS.pdf http://irep.iium.edu.my/90816/ http://phcogj.com/article/1626 http://dx.doi.org/10.5530/pj.2021.13.131 |
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| Summary: | Background: Eurycoma longifolia (E. longifolia) or Tongkat Ali is a tree that grows in southeast Asia, the
roots of which contain bioactive components that exhibit cytotoxic properties against various cancer cell
lines. However, no study has been conducted to relate the cytotoxic properties against nasopharyngeal
carcinoma (NPC), a type of cancer that shows poor prognosis for metastatic disease. The purpose of this
study was to determine whether the E. longifolia root extract exerts cytotoxic activity against nasopharyngeal
carcinoma (ORL-115) cell lines. Materials and Method: E. longifolia root extracts were obtained through
Soxhlet extraction method and by using two different solvents; ethanol and dicholoromethane. MTS assay
was used to evaluate the cytotoxic effect of the root extracts against ORL-115 cell line for three different
incubation time which were 24-hour, 48-hour and 72-hour. Results: Ethanol extract was significantly
more potent compared to DCM extract. Ethanol extract exhibited lower IC50 value compared to DCM
extract. The IC50 of ethanol extract were 232.1 μg/ml, 66.86 μg/ml and 42.6 μg/ml. Meanwhile the IC50 of
DCM extract were 678.87 μg/ml, 136.71 μg/ml, 73.72 μg/ml for 24-hour, 48-hour and 72-hour incubation
period respectively. The cytotoxic activity of both extracts increased as the incubation time prolonged.
The cytotoxic activity of ethanol extract at each incubation time was significantly different from DCM
extract except at 72 hours. Conclusion: E. longifolia root extracts exerted cytotoxic activity against the
nasopharyngeal carcinoma (ORL-115) cell line. Ethanol extract exhibited lower IC50 value compared to
DCM extract. The cytotoxic activity of both extracts were dose dependent and time dependent.
Key words: E. longifolia, Cytotoxic activity, Nasopharyngeal carcinoma. |
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