Cover Image

Virulence-genes Rib and Bca in Serotypes of Group B Streptococcus (GBS) Isolated from Symptomatic Pregnant Women in East Coast Malaysia

INTRODUCTION: Group B streptococcus (GBS) is a leading cause of maternally-acquired invasive infections in neonates. Nowadays maternal immunization is of utmost demand for prevention of these infections. We undertook capsular serotyping and virulence factor genes identification for local GBS isolate...

Full description

Saved in:
Bibliographic Details
Main Authors: Bahez, Ayesha, Mustafa Mahmoud, Mohammed Imad Al-Deen, Hamzah, Hairul Aini, Wahid, Hanan Hamimi
Format: Article
Language:English
English
Published: 2021
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/90764/7/90764_Virulence-genes%20Rib%20and%20Bca_Scopus.pdf
http://irep.iium.edu.my/90764/8/90764_Virulence-genes%20Rib%20and%20Bca.pdf
http://irep.iium.edu.my/90764/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.iium.irep.90764
record_format dspace
spelling my.iium.irep.907642021-08-12T01:17:36Z http://irep.iium.edu.my/90764/ Virulence-genes Rib and Bca in Serotypes of Group B Streptococcus (GBS) Isolated from Symptomatic Pregnant Women in East Coast Malaysia Bahez, Ayesha Mustafa Mahmoud, Mohammed Imad Al-Deen Hamzah, Hairul Aini Wahid, Hanan Hamimi R Medicine (General) INTRODUCTION: Group B streptococcus (GBS) is a leading cause of maternally-acquired invasive infections in neonates. Nowadays maternal immunization is of utmost demand for prevention of these infections. We undertook capsular serotyping and virulence factor genes identification for local GBS isolates as a pilot study, to identify potential candidates to propagate vaccine development. MATERIALS AND METHODS: This is a descriptive lab -based study to determine GBS serotypes and presence of genes coding virulence factors bca and rib in isolates obtained from symptomatic pregnant women in Hospital Tengku Ampuan Afzan, Kuantan, Pahang, Malaysia. Sixty-two GBS isolates from high vaginal swabs were collected. Latex agglutination test was performed to determine GBS serotypes. Real-time PCR was done to determine the presence of virulence genes. RESULTS: Of the 62 GBS isolates, 77.4% were serologically typeable, and 22.6% were non -typeable. Serotypes Ia and Ib (16.1% each) were the most common capsular types, followed by II, V, and VII (9.7% each), III (8.1%), VI (6.5%), and VIII (1.6 %). Furthermore, 67.7% of the isolates harboured the rib gene while 98.4% possessed the bca gene. CONCLUSION: The five known prevalent serotypes worldwide, do not match the CPS distribution in symptomatic pregnant women in Kuantan. However, the frequency of virulence genes rib and bca is high among our isolates, which if confirmed by further bigger and wider studies makes the proteinaceous vaccine, N-terminal domains of Rib and AlpC a suitable candidate for GBS prevention in this geographical area. 2021-07-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/90764/7/90764_Virulence-genes%20Rib%20and%20Bca_Scopus.pdf application/pdf en http://irep.iium.edu.my/90764/8/90764_Virulence-genes%20Rib%20and%20Bca.pdf Bahez, Ayesha and Mustafa Mahmoud, Mohammed Imad Al-Deen and Hamzah, Hairul Aini and Wahid, Hanan Hamimi (2021) Virulence-genes Rib and Bca in Serotypes of Group B Streptococcus (GBS) Isolated from Symptomatic Pregnant Women in East Coast Malaysia. IIUM Medical Journal Malaysia, 20 (3). pp. 118-126. ISSN 1823-4631 E-ISSN 2735-2285
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic R Medicine (General)
spellingShingle R Medicine (General)
Bahez, Ayesha
Mustafa Mahmoud, Mohammed Imad Al-Deen
Hamzah, Hairul Aini
Wahid, Hanan Hamimi
Virulence-genes Rib and Bca in Serotypes of Group B Streptococcus (GBS) Isolated from Symptomatic Pregnant Women in East Coast Malaysia
description INTRODUCTION: Group B streptococcus (GBS) is a leading cause of maternally-acquired invasive infections in neonates. Nowadays maternal immunization is of utmost demand for prevention of these infections. We undertook capsular serotyping and virulence factor genes identification for local GBS isolates as a pilot study, to identify potential candidates to propagate vaccine development. MATERIALS AND METHODS: This is a descriptive lab -based study to determine GBS serotypes and presence of genes coding virulence factors bca and rib in isolates obtained from symptomatic pregnant women in Hospital Tengku Ampuan Afzan, Kuantan, Pahang, Malaysia. Sixty-two GBS isolates from high vaginal swabs were collected. Latex agglutination test was performed to determine GBS serotypes. Real-time PCR was done to determine the presence of virulence genes. RESULTS: Of the 62 GBS isolates, 77.4% were serologically typeable, and 22.6% were non -typeable. Serotypes Ia and Ib (16.1% each) were the most common capsular types, followed by II, V, and VII (9.7% each), III (8.1%), VI (6.5%), and VIII (1.6 %). Furthermore, 67.7% of the isolates harboured the rib gene while 98.4% possessed the bca gene. CONCLUSION: The five known prevalent serotypes worldwide, do not match the CPS distribution in symptomatic pregnant women in Kuantan. However, the frequency of virulence genes rib and bca is high among our isolates, which if confirmed by further bigger and wider studies makes the proteinaceous vaccine, N-terminal domains of Rib and AlpC a suitable candidate for GBS prevention in this geographical area.
format Article
author Bahez, Ayesha
Mustafa Mahmoud, Mohammed Imad Al-Deen
Hamzah, Hairul Aini
Wahid, Hanan Hamimi
author_facet Bahez, Ayesha
Mustafa Mahmoud, Mohammed Imad Al-Deen
Hamzah, Hairul Aini
Wahid, Hanan Hamimi
author_sort Bahez, Ayesha
title Virulence-genes Rib and Bca in Serotypes of Group B Streptococcus (GBS) Isolated from Symptomatic Pregnant Women in East Coast Malaysia
title_short Virulence-genes Rib and Bca in Serotypes of Group B Streptococcus (GBS) Isolated from Symptomatic Pregnant Women in East Coast Malaysia
title_full Virulence-genes Rib and Bca in Serotypes of Group B Streptococcus (GBS) Isolated from Symptomatic Pregnant Women in East Coast Malaysia
title_fullStr Virulence-genes Rib and Bca in Serotypes of Group B Streptococcus (GBS) Isolated from Symptomatic Pregnant Women in East Coast Malaysia
title_full_unstemmed Virulence-genes Rib and Bca in Serotypes of Group B Streptococcus (GBS) Isolated from Symptomatic Pregnant Women in East Coast Malaysia
title_sort virulence-genes rib and bca in serotypes of group b streptococcus (gbs) isolated from symptomatic pregnant women in east coast malaysia
publishDate 2021
url http://irep.iium.edu.my/90764/7/90764_Virulence-genes%20Rib%20and%20Bca_Scopus.pdf
http://irep.iium.edu.my/90764/8/90764_Virulence-genes%20Rib%20and%20Bca.pdf
http://irep.iium.edu.my/90764/
_version_ 1709667142075416576
score 13.211869

Similar Items