Antibacterial composition of bioautographic fractions, characteristics, and stability of Carica papaya seed extract
The present work aimed to evaluate the potential of Carica papaya seed extract (CPSE) as an antibacterial agent against Salmonella Enteritidis, Bacillus cereus, Vibrio vulnificus, and Proteus mirabilis. The bioautography of the CPSE on thin-layer chromatography (TLC) plates was performed, followe...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universiti Putra Malaysia
2021
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/90636/13/90636_Antibacterial%20composition%20of%20bioautographic%20fractions.pdf http://irep.iium.edu.my/90636/ http://www.ifrj.upm.edu.my/28%20(03)%202021/04%20-%20IFRJ19934.R1.pdf |
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| Summary: | The present work aimed to evaluate the potential of Carica papaya seed extract (CPSE) as an
antibacterial agent against Salmonella Enteritidis, Bacillus cereus, Vibrio vulnificus, and
Proteus mirabilis. The bioautography of the CPSE on thin-layer chromatography (TLC) plates
was performed, followed by fractionation of the CPSE by column chromatography using
hexane:ethyl acetate (1:1) eluent. Minimum Inhibitory Concentration (MIC), toxicity,
composition, and stability of the crude, fractions, and sub-fractions of the CPSE were
evaluated. The bioautographic fractions of the CPSE at MIC of 5.63 mg/mL had shown that
hexane:ethyl acetate (1:1) fraction and its sub-fraction 3C (Rf = 0.94 ± 0.03) demonstrated the
equivalent MIC value (5.63 mg/mL) with the crude CPSE. However, the hexane:ethyl acetate
(1:1) fraction and sub-fraction 3C had higher toxicity (LC50 = 1.797 ± 0.305 and 0.332 ± 0.059
mg/mL, respectively) than the crude CPSE (LC50 = 5.505 ± 0.718 mg/mL). Thus, only the
crude CPSE was subjected to stability study. The dominant cis-vaccenic acid in sub-fraction
3C demonstrated the lowest MIC against B. cereus (1.41 mg/mL), P. mirabilis (1.41 mg/mL),
and S. Enteritidis (0.70 mg/mL) in its pure form. Hence, these results signified the potency of
the cis-vaccenic acid as an antibacterial compound from the CPSE. The stability study of the
crude CPSE solution showed that at MIC of 5.63 mg/mL, the crude CPSE solution acted as a
potent antibacterial agent in acidic condition (pH 4), water activity (Aw) < 0.950, and
temperature < 40°C. |
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