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Assessment of concordance between immunohistochemistry and MSI analysis and their association with clinicopathological features in Malaysian colorectal cancer patients

Introduction: Colorectal cancer (CRC) is the second most common tumour in Malaysia. Universal screening for the identification of microsatellite instability/mismatch repair (MSI/MMR) status in CRC patients is recommended by several guidelines. The detection of MSI/MMR status in CRC patients is not...

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Main Authors: Faizee, Muhammad Ishaque, Hamdan, Asmah Hanim, Abdullah, Nor Zamzila, A. Talib, Norlelawati
Format: Article
Language:English
English
English
Published: KAHS 2021
Subjects:
RB Pathology
Online Access:http://irep.iium.edu.my/90518/7/View%20of%20ASSESSMENT%20OF%20CONCORDANCE%20BETWEEN%20IMMUNOHISTOCHEMISTRY%20AND%20MSI%20ANALYSIS%20AND%20THEIR%20ASSOCIATION%20WITH%20CLINICOPATHOLOGICAL%20FEATURES%20IN%20MALAYSIAN%20COLORECTAL%20CANCER%20PATIENTS.html
http://irep.iium.edu.my/90518/1/abstract%20KRD%202020.pdf
http://irep.iium.edu.my/90518/8/90518_Assessment%20of%20concordance%20between%20immunohistochemistry.pdf
http://irep.iium.edu.my/90518/
https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/565/531
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spelling my.iium.irep.905182021-07-05T06:44:52Z http://irep.iium.edu.my/90518/ Assessment of concordance between immunohistochemistry and MSI analysis and their association with clinicopathological features in Malaysian colorectal cancer patients Faizee, Muhammad Ishaque Hamdan, Asmah Hanim Abdullah, Nor Zamzila A. Talib, Norlelawati RB Pathology Introduction: Colorectal cancer (CRC) is the second most common tumour in Malaysia. Universal screening for the identification of microsatellite instability/mismatch repair (MSI/MMR) status in CRC patients is recommended by several guidelines. The detection of MSI/MMR status in CRC patients is not only essential to identify Lynch Syndrome (LS), but it also has predictive and prognostic values. The study aimed to investigate the MMR and MSI status among CRC patients as well as to assess the concordance between immunohistochemistry (IHC) and MSI analysis. Method: Formalin-fixed paraffin-embedded (FFPE) tissue blocks of 123 CRC patients were retrieved for the years 2017-2018. For IHC and MSI analysis, EnVisionTM FLEX, Mini Kit, High PH, and MSI Analysis System 1.2 (Promega) were utilized, respectively. MSI analysis was performed on selected deficient mismatch repair (dMMR) and proficient mismatch repair (pMMR) cases. Results: IHC detected 11.4% (14 out of 123) patients as dMMR and 88.6% (109 out of 123) as pMMR. MSI analysis identified 26% (13 out of 50) patients as MSI-H, 6% (3 out of 50) patients as MSI-L, and 64% (32 out of 50) patients as MSS. Both the IHC and MSI analysis showed perfect agreement (0.896, Kappa value) for the recognition of dMMR or MSI-H CRC patients while demonstrating only 4% (2 out of 50) discordant results. Almost all dMMR patients detected by IHC were recognized by MSI analysis as MSI-H except one. Conclusion: The significant prevalence of dMMR in the current cohort supports the recommendation that the assessment of MSI/MMR status should be addressed in CRC patients. The selection of the choice method may be based on the availability of expertise and equipment. Since IHC is an affordable, reproducible, and readily available in most histopathological laboratories, it can be used as a primary screening test to detect MSI/MMR status in CRC patients. KAHS 2021-08-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/90518/7/View%20of%20ASSESSMENT%20OF%20CONCORDANCE%20BETWEEN%20IMMUNOHISTOCHEMISTRY%20AND%20MSI%20ANALYSIS%20AND%20THEIR%20ASSOCIATION%20WITH%20CLINICOPATHOLOGICAL%20FEATURES%20IN%20MALAYSIAN%20COLORECTAL%20CANCER%20PATIENTS.html application/pdf en http://irep.iium.edu.my/90518/1/abstract%20KRD%202020.pdf application/pdf en http://irep.iium.edu.my/90518/8/90518_Assessment%20of%20concordance%20between%20immunohistochemistry.pdf Faizee, Muhammad Ishaque and Hamdan, Asmah Hanim and Abdullah, Nor Zamzila and A. Talib, Norlelawati (2021) Assessment of concordance between immunohistochemistry and MSI analysis and their association with clinicopathological features in Malaysian colorectal cancer patients. International Journal of Allied Health Sciences, 5 (1). p. 2128. E-ISSN 2600-8491 https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/565/531
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic RB Pathology
spellingShingle RB Pathology
Faizee, Muhammad Ishaque
Hamdan, Asmah Hanim
Abdullah, Nor Zamzila
A. Talib, Norlelawati
Assessment of concordance between immunohistochemistry and MSI analysis and their association with clinicopathological features in Malaysian colorectal cancer patients
description Introduction: Colorectal cancer (CRC) is the second most common tumour in Malaysia. Universal screening for the identification of microsatellite instability/mismatch repair (MSI/MMR) status in CRC patients is recommended by several guidelines. The detection of MSI/MMR status in CRC patients is not only essential to identify Lynch Syndrome (LS), but it also has predictive and prognostic values. The study aimed to investigate the MMR and MSI status among CRC patients as well as to assess the concordance between immunohistochemistry (IHC) and MSI analysis. Method: Formalin-fixed paraffin-embedded (FFPE) tissue blocks of 123 CRC patients were retrieved for the years 2017-2018. For IHC and MSI analysis, EnVisionTM FLEX, Mini Kit, High PH, and MSI Analysis System 1.2 (Promega) were utilized, respectively. MSI analysis was performed on selected deficient mismatch repair (dMMR) and proficient mismatch repair (pMMR) cases. Results: IHC detected 11.4% (14 out of 123) patients as dMMR and 88.6% (109 out of 123) as pMMR. MSI analysis identified 26% (13 out of 50) patients as MSI-H, 6% (3 out of 50) patients as MSI-L, and 64% (32 out of 50) patients as MSS. Both the IHC and MSI analysis showed perfect agreement (0.896, Kappa value) for the recognition of dMMR or MSI-H CRC patients while demonstrating only 4% (2 out of 50) discordant results. Almost all dMMR patients detected by IHC were recognized by MSI analysis as MSI-H except one. Conclusion: The significant prevalence of dMMR in the current cohort supports the recommendation that the assessment of MSI/MMR status should be addressed in CRC patients. The selection of the choice method may be based on the availability of expertise and equipment. Since IHC is an affordable, reproducible, and readily available in most histopathological laboratories, it can be used as a primary screening test to detect MSI/MMR status in CRC patients.
format Article
author Faizee, Muhammad Ishaque
Hamdan, Asmah Hanim
Abdullah, Nor Zamzila
A. Talib, Norlelawati
author_facet Faizee, Muhammad Ishaque
Hamdan, Asmah Hanim
Abdullah, Nor Zamzila
A. Talib, Norlelawati
author_sort Faizee, Muhammad Ishaque
title Assessment of concordance between immunohistochemistry and MSI analysis and their association with clinicopathological features in Malaysian colorectal cancer patients
title_short Assessment of concordance between immunohistochemistry and MSI analysis and their association with clinicopathological features in Malaysian colorectal cancer patients
title_full Assessment of concordance between immunohistochemistry and MSI analysis and their association with clinicopathological features in Malaysian colorectal cancer patients
title_fullStr Assessment of concordance between immunohistochemistry and MSI analysis and their association with clinicopathological features in Malaysian colorectal cancer patients
title_full_unstemmed Assessment of concordance between immunohistochemistry and MSI analysis and their association with clinicopathological features in Malaysian colorectal cancer patients
title_sort assessment of concordance between immunohistochemistry and msi analysis and their association with clinicopathological features in malaysian colorectal cancer patients
publisher KAHS
publishDate 2021
url http://irep.iium.edu.my/90518/7/View%20of%20ASSESSMENT%20OF%20CONCORDANCE%20BETWEEN%20IMMUNOHISTOCHEMISTRY%20AND%20MSI%20ANALYSIS%20AND%20THEIR%20ASSOCIATION%20WITH%20CLINICOPATHOLOGICAL%20FEATURES%20IN%20MALAYSIAN%20COLORECTAL%20CANCER%20PATIENTS.html
http://irep.iium.edu.my/90518/1/abstract%20KRD%202020.pdf
http://irep.iium.edu.my/90518/8/90518_Assessment%20of%20concordance%20between%20immunohistochemistry.pdf
http://irep.iium.edu.my/90518/
https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/565/531
_version_ 1705056520747614208
score 13.211869

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