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Hypotensive effects of aqueous extract of Eugenia polyantha leaves are partly mediated via cholinergic receptor

Eugenia polyantha leaves, a popular fresh salad (‘ulam’) used by the Kelantanese has been claimed as a cure for hypertension. In this study, the hypotensive effects of aqueous extract of Eugenia polyantha leaves (AEEP) on the anaesthetized male normotensive Wistar-Kyoto rats were described. Increasi...

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Main Authors: Ismail, Azlini, Sulaiman, Siti Amrah, Mohamed, Mahaneem, Syed Sahil Jamalullail, Syed Mohsin
Format: Article
Language:English
Published: National Library of Malaysia 2011
Subjects:
RM Therapeutics. Pharmacology
RM147 Administration of Drugs and Other Therapeutic Agents
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/90204/1/90204_Hypotensive%20effects%20of%20aqueous%20extract%20of%20Eugenia.pdf
http://irep.iium.edu.my/90204/
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spelling my.iium.irep.902042021-06-21T14:25:08Z http://irep.iium.edu.my/90204/ Hypotensive effects of aqueous extract of Eugenia polyantha leaves are partly mediated via cholinergic receptor Ismail, Azlini Sulaiman, Siti Amrah Mohamed, Mahaneem Syed Sahil Jamalullail, Syed Mohsin RM Therapeutics. Pharmacology RM147 Administration of Drugs and Other Therapeutic Agents RS Pharmacy and materia medica Eugenia polyantha leaves, a popular fresh salad (‘ulam’) used by the Kelantanese has been claimed as a cure for hypertension. In this study, the hypotensive effects of aqueous extract of Eugenia polyantha leaves (AEEP) on the anaesthetized male normotensive Wistar-Kyoto rats were described. Increasing intravenous doses of AEEP (0.01, 0.1, 1, 10 and 100 mg/kg) significantly reduced the mean arterial blood pressure (MAP) of twelve animals by 6.06 ± 4.1 %, 8.02 ± 3.9 %, 6.35 ± 0.6 %, 9.34 ± 1.6 % and 30.19 ± 2.0 % (Wilcoxon-Signed Rank test or WSR, p<0.01) respectively. However, only reductions in MAP, by the doses of 0.1, 1, 10 and 100 mg/kg of AEEP and by the positive control (5 µg/kg acetylcholine, 37.87 ± 1.7 %) were significantly higher than the reduction of MAP by the negative control (0.9 % normal saline, 5.372 ± 0.5 %) (WSR test, p<0.05). From the dose-response curve, the ED50 value for hypotensive effects of AEEP was 35.5 mg/kg. Subsequently, a pharmacological antagonistic study was carried out either of pre-treatment with propranolol (2 mg/kg) or by pre-treatment with atropine (2 mg/kg). Pre-treatment with atropine (2 mg/kg) significantly reduced the hypotensive effect of 100 mg/kg of AEEP (WSR test, p<0.05) whilst pre-treatment with propranolol (2 mg/kg) does not significantly reduced the hypotensive effect of 100 mg/kg AEEP (WSR test, p>0.05). This attenuation suggested that the hypotensive effect of AEEP may be partly mediated by cholinergic but not by β-adrenergic receptor pathways. National Library of Malaysia 2011-06-22 Article PeerReviewed application/pdf en http://irep.iium.edu.my/90204/1/90204_Hypotensive%20effects%20of%20aqueous%20extract%20of%20Eugenia.pdf Ismail, Azlini and Sulaiman, Siti Amrah and Mohamed, Mahaneem and Syed Sahil Jamalullail, Syed Mohsin (2011) Hypotensive effects of aqueous extract of Eugenia polyantha leaves are partly mediated via cholinergic receptor. Conference Proceedings 16th National Conference on Medical and Heath Sciences. pp. 20-28. ISSN 978-967-5547-35-5
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RM Therapeutics. Pharmacology
RM147 Administration of Drugs and Other Therapeutic Agents
RS Pharmacy and materia medica
spellingShingle RM Therapeutics. Pharmacology
RM147 Administration of Drugs and Other Therapeutic Agents
RS Pharmacy and materia medica
Ismail, Azlini
Sulaiman, Siti Amrah
Mohamed, Mahaneem
Syed Sahil Jamalullail, Syed Mohsin
Hypotensive effects of aqueous extract of Eugenia polyantha leaves are partly mediated via cholinergic receptor
description Eugenia polyantha leaves, a popular fresh salad (‘ulam’) used by the Kelantanese has been claimed as a cure for hypertension. In this study, the hypotensive effects of aqueous extract of Eugenia polyantha leaves (AEEP) on the anaesthetized male normotensive Wistar-Kyoto rats were described. Increasing intravenous doses of AEEP (0.01, 0.1, 1, 10 and 100 mg/kg) significantly reduced the mean arterial blood pressure (MAP) of twelve animals by 6.06 ± 4.1 %, 8.02 ± 3.9 %, 6.35 ± 0.6 %, 9.34 ± 1.6 % and 30.19 ± 2.0 % (Wilcoxon-Signed Rank test or WSR, p<0.01) respectively. However, only reductions in MAP, by the doses of 0.1, 1, 10 and 100 mg/kg of AEEP and by the positive control (5 µg/kg acetylcholine, 37.87 ± 1.7 %) were significantly higher than the reduction of MAP by the negative control (0.9 % normal saline, 5.372 ± 0.5 %) (WSR test, p<0.05). From the dose-response curve, the ED50 value for hypotensive effects of AEEP was 35.5 mg/kg. Subsequently, a pharmacological antagonistic study was carried out either of pre-treatment with propranolol (2 mg/kg) or by pre-treatment with atropine (2 mg/kg). Pre-treatment with atropine (2 mg/kg) significantly reduced the hypotensive effect of 100 mg/kg of AEEP (WSR test, p<0.05) whilst pre-treatment with propranolol (2 mg/kg) does not significantly reduced the hypotensive effect of 100 mg/kg AEEP (WSR test, p>0.05). This attenuation suggested that the hypotensive effect of AEEP may be partly mediated by cholinergic but not by β-adrenergic receptor pathways.
format Article
author Ismail, Azlini
Sulaiman, Siti Amrah
Mohamed, Mahaneem
Syed Sahil Jamalullail, Syed Mohsin
author_facet Ismail, Azlini
Sulaiman, Siti Amrah
Mohamed, Mahaneem
Syed Sahil Jamalullail, Syed Mohsin
author_sort Ismail, Azlini
title Hypotensive effects of aqueous extract of Eugenia polyantha leaves are partly mediated via cholinergic receptor
title_short Hypotensive effects of aqueous extract of Eugenia polyantha leaves are partly mediated via cholinergic receptor
title_full Hypotensive effects of aqueous extract of Eugenia polyantha leaves are partly mediated via cholinergic receptor
title_fullStr Hypotensive effects of aqueous extract of Eugenia polyantha leaves are partly mediated via cholinergic receptor
title_full_unstemmed Hypotensive effects of aqueous extract of Eugenia polyantha leaves are partly mediated via cholinergic receptor
title_sort hypotensive effects of aqueous extract of eugenia polyantha leaves are partly mediated via cholinergic receptor
publisher National Library of Malaysia
publishDate 2011
url http://irep.iium.edu.my/90204/1/90204_Hypotensive%20effects%20of%20aqueous%20extract%20of%20Eugenia.pdf
http://irep.iium.edu.my/90204/
_version_ 1703960211543293952
score 13.211869

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