Staff View: COVID-19 outbreak in Malaysia: decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang

COVID-19 outbreak in Malaysia: decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang

SARS-CoV-2 is a very transmissible and pathogenic coronavirus which detected in Malaysia in January 2020. Nevertheless, the sample from Malaysia is still under-sequenced. Hence lacking clarity of the circulating strain in Malaysia leads to a deadlock in understanding the virus infectivity. This stud...

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Main Authors:	Mat Yassim, Aini Syahida, Farida Asras, Mohd Fazli, Gazali, Ahmad Mahfuz, Martin S, Martial Coba, Zainulabid, Ummu Afeera, Ahmad, Hajar Fauzan
Format:	Article
Language:	English
Published:	2021
Subjects:	RC Internal medicine RC111 Infectious and Parasitic Diseases
Online Access:	http://irep.iium.edu.my/90189/7/90189_COVID-19%20outbreak%20in%20Malaysia%20decoding%20D614G%20mutation%20of%20SARS-CoV-2.pdf http://irep.iium.edu.my/90189/ https://pubmed.ncbi.nlm.nih.gov/33680867/
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spelling	my.iium.irep.901892021-07-09T09:39:06Z http://irep.iium.edu.my/90189/ COVID-19 outbreak in Malaysia: decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang Mat Yassim, Aini Syahida Farida Asras, Mohd Fazli Gazali, Ahmad Mahfuz Martin S, Martial Coba Zainulabid, Ummu Afeera Ahmad, Hajar Fauzan RC Internal medicine RC111 Infectious and Parasitic Diseases SARS-CoV-2 is a very transmissible and pathogenic coronavirus which detected in Malaysia in January 2020. Nevertheless, the sample from Malaysia is still under-sequenced. Hence lacking clarity of the circulating strain in Malaysia leads to a deadlock in understanding the virus infectivity. This study aimed to investigate the genome identity of circulating COVID-19 strains in Pahang and understand disease epidemiology during the pandemic. This study leveraged high-throughput sequencing analysis for the whole genome sequencing and implemented bioinformatic technique for the analysis. Here we reported that the virus with D614G mutation in Spike protein circulates in a few Malaysia states before the Sivagangga cluster announced in Kedah in July 2020. This mutated virus includes our virus sample isolated in April 2020 from an asymptomatic patient in Pahang. Based on the phylogenetic analysis, we discovered the origin of our sample Pahang/IIUM91 was not related to Sivagangga cluster. Here, we have generated 3D structure model of Pahang/IIUM91 Spike protein. D614G mutation in Pahang/IIUM91 Spike protein increases viral stability and flexibility, hence render higher infectivity. Collectively, our results suggest for the establishment of a complete SARS-CoV-2 genome database in Malaysia. Hence, more research should be established to learn the behaviour of this virus. 2021-02 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/90189/7/90189_COVID-19%20outbreak%20in%20Malaysia%20decoding%20D614G%20mutation%20of%20SARS-CoV-2.pdf Mat Yassim, Aini Syahida and Farida Asras, Mohd Fazli and Gazali, Ahmad Mahfuz and Martin S, Martial Coba and Zainulabid, Ummu Afeera and Ahmad, Hajar Fauzan (2021) COVID-19 outbreak in Malaysia: decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang. Materials Today: Proceedings. E-ISSN 2214-7853 (In Press) https://pubmed.ncbi.nlm.nih.gov/33680867/
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	RC Internal medicine RC111 Infectious and Parasitic Diseases
spellingShingle	RC Internal medicine RC111 Infectious and Parasitic Diseases Mat Yassim, Aini Syahida Farida Asras, Mohd Fazli Gazali, Ahmad Mahfuz Martin S, Martial Coba Zainulabid, Ummu Afeera Ahmad, Hajar Fauzan COVID-19 outbreak in Malaysia: decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang
description	SARS-CoV-2 is a very transmissible and pathogenic coronavirus which detected in Malaysia in January 2020. Nevertheless, the sample from Malaysia is still under-sequenced. Hence lacking clarity of the circulating strain in Malaysia leads to a deadlock in understanding the virus infectivity. This study aimed to investigate the genome identity of circulating COVID-19 strains in Pahang and understand disease epidemiology during the pandemic. This study leveraged high-throughput sequencing analysis for the whole genome sequencing and implemented bioinformatic technique for the analysis. Here we reported that the virus with D614G mutation in Spike protein circulates in a few Malaysia states before the Sivagangga cluster announced in Kedah in July 2020. This mutated virus includes our virus sample isolated in April 2020 from an asymptomatic patient in Pahang. Based on the phylogenetic analysis, we discovered the origin of our sample Pahang/IIUM91 was not related to Sivagangga cluster. Here, we have generated 3D structure model of Pahang/IIUM91 Spike protein. D614G mutation in Pahang/IIUM91 Spike protein increases viral stability and flexibility, hence render higher infectivity. Collectively, our results suggest for the establishment of a complete SARS-CoV-2 genome database in Malaysia. Hence, more research should be established to learn the behaviour of this virus.
format	Article
author	Mat Yassim, Aini Syahida Farida Asras, Mohd Fazli Gazali, Ahmad Mahfuz Martin S, Martial Coba Zainulabid, Ummu Afeera Ahmad, Hajar Fauzan
author_facet	Mat Yassim, Aini Syahida Farida Asras, Mohd Fazli Gazali, Ahmad Mahfuz Martin S, Martial Coba Zainulabid, Ummu Afeera Ahmad, Hajar Fauzan
author_sort	Mat Yassim, Aini Syahida
title	COVID-19 outbreak in Malaysia: decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang
title_short	COVID-19 outbreak in Malaysia: decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang
title_full	COVID-19 outbreak in Malaysia: decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang
title_fullStr	COVID-19 outbreak in Malaysia: decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang
title_full_unstemmed	COVID-19 outbreak in Malaysia: decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang
title_sort	covid-19 outbreak in malaysia: decoding d614g mutation of sars-cov-2 virus isolated from an asymptomatic case in pahang
publishDate	2021
url	http://irep.iium.edu.my/90189/7/90189_COVID-19%20outbreak%20in%20Malaysia%20decoding%20D614G%20mutation%20of%20SARS-CoV-2.pdf http://irep.iium.edu.my/90189/ https://pubmed.ncbi.nlm.nih.gov/33680867/
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score	13.211869

COVID-19 outbreak in Malaysia: decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang

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