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In silico CHARACTERIZATION OF UGT76G1 PROTEIN IN Stevia rebaudiana BERTONI ACCESSIONS MS007

Stevia rebaudiana is a member of the Asteraceae family which is being consumed as an artificial sweetener. Stevia has been identified to be safe for human consumption and has been used as an alternative sweetener for diabetic and obese patients. This study used an in silico analysis of the transcrip...

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Main Authors: Samsulrizal, Nurul Hidayah, Zainuddin, Zarina, Meenakshi Sundram, Tamil Chelvan
Format: Conference or Workshop Item
Language:English
English
English
English
English
Published: 2021
Subjects:
S Agriculture (General)
Online Access:http://irep.iium.edu.my/90114/1/ABSTRACT-ICMBB.pdf
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spelling my.iium.irep.901142021-06-18T03:18:24Z http://irep.iium.edu.my/90114/ In silico CHARACTERIZATION OF UGT76G1 PROTEIN IN Stevia rebaudiana BERTONI ACCESSIONS MS007 Samsulrizal, Nurul Hidayah Zainuddin, Zarina Meenakshi Sundram, Tamil Chelvan S Agriculture (General) Stevia rebaudiana is a member of the Asteraceae family which is being consumed as an artificial sweetener. Stevia has been identified to be safe for human consumption and has been used as an alternative sweetener for diabetic and obese patients. This study used an in silico analysis of the transcriptome dataset of Stevia rebaudiana accession MS007 to describe the structure and gene content involved in the production of UGT76G1 protein. Homologous search using BLASTp resulting high similarity to UDP-glycosyltransferase 76G1-like Helianthus annuus (ID: XP_021973845.1) as the highest percentage of identity. Protein family search using InterPro showed the presence and entry of IPR002213 available at positions 89 to 246. The phylogenetic tree was constructed by selecting 14 out of 100 protein sequences from BLASTp using MEGA X software. The phylogenetic tree analysis showed the same protein family which is Asteraceae. The protein structure prediction of primary, secondary and tertiary of UGT76G1 protein was computed using ProtParam ExPasy, PSIPRED and Phyre2. The tertiary structure prediction of UGT76G1 protein scored 100.0% confidence by the single highest scoring template and coverage of 98% and the dimension of the model is (Å) of X: 52.453, Y: 61.270, and Z: 48.102. The findings of this study will lead to a deeper understanding of the characteristics of UDP-glycosyltransferase 76G1 (S. rebaudiana MS007) and enhance target recognition mechanisms, resulting in a better understanding of protein-protein interaction in Stevia rebaudiana MS007. 2021 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/90114/1/ABSTRACT-ICMBB.pdf application/pdf en http://irep.iium.edu.my/90114/2/Poster%20Nor%20Iswani%20Mokthar.jpg application/pdf en http://irep.iium.edu.my/90114/3/watch_v%3DSet056Ncdqk%26ab_channel%3D4thICMBB2021 application/pdf en http://irep.iium.edu.my/90114/13/Programme%20Book_4thICMBB2021%20%281%29.pdf application/pdf en http://irep.iium.edu.my/90114/14/ICMBB2021%20Participation%20eCert.pdf Samsulrizal, Nurul Hidayah and Zainuddin, Zarina and Meenakshi Sundram, Tamil Chelvan (2021) In silico CHARACTERIZATION OF UGT76G1 PROTEIN IN Stevia rebaudiana BERTONI ACCESSIONS MS007. In: 4th International Conference on Molecular Biology and Biotechnology (4th ICMBB2021), Kuala Lumpur, Malaysia, Virtual Seminar. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
English
English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Samsulrizal, Nurul Hidayah
Zainuddin, Zarina
Meenakshi Sundram, Tamil Chelvan
In silico CHARACTERIZATION OF UGT76G1 PROTEIN IN Stevia rebaudiana BERTONI ACCESSIONS MS007
description Stevia rebaudiana is a member of the Asteraceae family which is being consumed as an artificial sweetener. Stevia has been identified to be safe for human consumption and has been used as an alternative sweetener for diabetic and obese patients. This study used an in silico analysis of the transcriptome dataset of Stevia rebaudiana accession MS007 to describe the structure and gene content involved in the production of UGT76G1 protein. Homologous search using BLASTp resulting high similarity to UDP-glycosyltransferase 76G1-like Helianthus annuus (ID: XP_021973845.1) as the highest percentage of identity. Protein family search using InterPro showed the presence and entry of IPR002213 available at positions 89 to 246. The phylogenetic tree was constructed by selecting 14 out of 100 protein sequences from BLASTp using MEGA X software. The phylogenetic tree analysis showed the same protein family which is Asteraceae. The protein structure prediction of primary, secondary and tertiary of UGT76G1 protein was computed using ProtParam ExPasy, PSIPRED and Phyre2. The tertiary structure prediction of UGT76G1 protein scored 100.0% confidence by the single highest scoring template and coverage of 98% and the dimension of the model is (Å) of X: 52.453, Y: 61.270, and Z: 48.102. The findings of this study will lead to a deeper understanding of the characteristics of UDP-glycosyltransferase 76G1 (S. rebaudiana MS007) and enhance target recognition mechanisms, resulting in a better understanding of protein-protein interaction in Stevia rebaudiana MS007.
format Conference or Workshop Item
author Samsulrizal, Nurul Hidayah
Zainuddin, Zarina
Meenakshi Sundram, Tamil Chelvan
author_facet Samsulrizal, Nurul Hidayah
Zainuddin, Zarina
Meenakshi Sundram, Tamil Chelvan
author_sort Samsulrizal, Nurul Hidayah
title In silico CHARACTERIZATION OF UGT76G1 PROTEIN IN Stevia rebaudiana BERTONI ACCESSIONS MS007
title_short In silico CHARACTERIZATION OF UGT76G1 PROTEIN IN Stevia rebaudiana BERTONI ACCESSIONS MS007
title_full In silico CHARACTERIZATION OF UGT76G1 PROTEIN IN Stevia rebaudiana BERTONI ACCESSIONS MS007
title_fullStr In silico CHARACTERIZATION OF UGT76G1 PROTEIN IN Stevia rebaudiana BERTONI ACCESSIONS MS007
title_full_unstemmed In silico CHARACTERIZATION OF UGT76G1 PROTEIN IN Stevia rebaudiana BERTONI ACCESSIONS MS007
title_sort in silico characterization of ugt76g1 protein in stevia rebaudiana bertoni accessions ms007
publishDate 2021
url http://irep.iium.edu.my/90114/1/ABSTRACT-ICMBB.pdf
http://irep.iium.edu.my/90114/2/Poster%20Nor%20Iswani%20Mokthar.jpg
http://irep.iium.edu.my/90114/3/watch_v%3DSet056Ncdqk%26ab_channel%3D4thICMBB2021
http://irep.iium.edu.my/90114/13/Programme%20Book_4thICMBB2021%20%281%29.pdf
http://irep.iium.edu.my/90114/14/ICMBB2021%20Participation%20eCert.pdf
http://irep.iium.edu.my/90114/
_version_ 1703960205526564864
score 13.211869

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