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A high glucose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: Results of an in vitro investigation

Background: Glucose is associated with weight gain, which increases the risk of cancer. There is insufficient information on the effects of high glucose concentrations on cell lines. This study evaluated the viability patterns of human cancer and normal cell lines treated with glucose. Methods: Hu...

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Main Authors: Mazlan, Muhammad Alif, Mat Yusof, Afzan, Md Isa, Muhammad Lokman, ,
Format: Article
Language:English
Published: Universitas Indonesia 2020
Subjects:
Q Science (General)
R Medicine (General)
RT Nursing
Online Access:http://irep.iium.edu.my/89819/1/89819_A%20high%20glucose%20concentration%20is%20well%20tolerated%20by%20colorectal%20adenocarcinoma%20and%20melanoma%20cells.pdf
http://irep.iium.edu.my/89819/
https://scholarhub.ui.ac.id/mjhr/vol24/iss1/9/
https://doi.org/10.7454/msk.v24i1.1179
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spelling my.iium.irep.898192021-05-12T05:09:09Z http://irep.iium.edu.my/89819/ A high glucose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: Results of an in vitro investigation Mazlan, Muhammad Alif Mat Yusof, Afzan Md Isa, Muhammad Lokman , Q Science (General) R Medicine (General) RT Nursing Background: Glucose is associated with weight gain, which increases the risk of cancer. There is insufficient information on the effects of high glucose concentrations on cell lines. This study evaluated the viability patterns of human cancer and normal cell lines treated with glucose. Methods: Human gingival fibroblast (hGF), colorectal adenocarcinoma (HT29), and skin malignant melanoma (A375) cell lines were cultured and treated with additional glucose in three respective concentrations: 1 mg/ml, 5 mg/ml, and 10 mg/ml. Then, cell viability was measured using an MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide)-assay. The data were analyzed using the Statistical Package for the Social Sciences software. Results: The hGF cells’ percentage pattern of viability showed a rapid decline of nearly 95% on the third day of treatment. Both HT29 and A375 were able to survive in the high glucose condition when the cell viability percentage was above 100% on Day 5. The data exhibited significance; the p-value was less than 0.001. Conclusion: The high glucose concentration can be toxic to hGF. In addition, HT29 and A375 might be adaptive to the hyperglycaemic condition. Universitas Indonesia 2020-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/89819/1/89819_A%20high%20glucose%20concentration%20is%20well%20tolerated%20by%20colorectal%20adenocarcinoma%20and%20melanoma%20cells.pdf Mazlan, Muhammad Alif and Mat Yusof, Afzan and Md Isa, Muhammad Lokman and UNSPECIFIED (2020) A high glucose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: Results of an in vitro investigation. Makara Journal of Health Research, 24 (1). pp. 57-62. ISSN 2356-3664 E-ISSN 2356-3656 https://scholarhub.ui.ac.id/mjhr/vol24/iss1/9/ https://doi.org/10.7454/msk.v24i1.1179
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
R Medicine (General)
RT Nursing
spellingShingle Q Science (General)
R Medicine (General)
RT Nursing
Mazlan, Muhammad Alif
Mat Yusof, Afzan
Md Isa, Muhammad Lokman
,
A high glucose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: Results of an in vitro investigation
description Background: Glucose is associated with weight gain, which increases the risk of cancer. There is insufficient information on the effects of high glucose concentrations on cell lines. This study evaluated the viability patterns of human cancer and normal cell lines treated with glucose. Methods: Human gingival fibroblast (hGF), colorectal adenocarcinoma (HT29), and skin malignant melanoma (A375) cell lines were cultured and treated with additional glucose in three respective concentrations: 1 mg/ml, 5 mg/ml, and 10 mg/ml. Then, cell viability was measured using an MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide)-assay. The data were analyzed using the Statistical Package for the Social Sciences software. Results: The hGF cells’ percentage pattern of viability showed a rapid decline of nearly 95% on the third day of treatment. Both HT29 and A375 were able to survive in the high glucose condition when the cell viability percentage was above 100% on Day 5. The data exhibited significance; the p-value was less than 0.001. Conclusion: The high glucose concentration can be toxic to hGF. In addition, HT29 and A375 might be adaptive to the hyperglycaemic condition.
format Article
author Mazlan, Muhammad Alif
Mat Yusof, Afzan
Md Isa, Muhammad Lokman
,
author_facet Mazlan, Muhammad Alif
Mat Yusof, Afzan
Md Isa, Muhammad Lokman
,
author_sort Mazlan, Muhammad Alif
title A high glucose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: Results of an in vitro investigation
title_short A high glucose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: Results of an in vitro investigation
title_full A high glucose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: Results of an in vitro investigation
title_fullStr A high glucose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: Results of an in vitro investigation
title_full_unstemmed A high glucose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: Results of an in vitro investigation
title_sort high glucose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: results of an in vitro investigation
publisher Universitas Indonesia
publishDate 2020
url http://irep.iium.edu.my/89819/1/89819_A%20high%20glucose%20concentration%20is%20well%20tolerated%20by%20colorectal%20adenocarcinoma%20and%20melanoma%20cells.pdf
http://irep.iium.edu.my/89819/
https://scholarhub.ui.ac.id/mjhr/vol24/iss1/9/
https://doi.org/10.7454/msk.v24i1.1179
_version_ 1701162794429186048
score 13.211869

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