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Growth optimization of mangrove Streptomyces for enhancement of actinorhodin production

The family Streptomycetaceae, notably species in genus Streptomyces, have long been the subject of investigation due to their well-known ability to produce secondary metabolites. Even though thousands of secondary metabolites have been discovered from Streptomyces, less attention has been given in...

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Main Authors: Azizan, Nur Hafizah, Sharif, Faez, Zainal Abidin, Zaima Azira, Zainuddin, Zarina, Chowdhury, Ahmed Jalal Khan
Format: Article
Language:English
Published: 2021
Subjects:
QR Microbiology
Online Access:http://irep.iium.edu.my/89684/1/actinorhodin%20Azam%20full%20paper.pdf
http://irep.iium.edu.my/89684/
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spelling my.iium.irep.896842021-10-15T03:39:48Z http://irep.iium.edu.my/89684/ Growth optimization of mangrove Streptomyces for enhancement of actinorhodin production Azizan, Nur Hafizah Sharif, Faez Zainal Abidin, Zaima Azira Zainuddin, Zarina Chowdhury, Ahmed Jalal Khan QR Microbiology The family Streptomycetaceae, notably species in genus Streptomyces, have long been the subject of investigation due to their well-known ability to produce secondary metabolites. Even though thousands of secondary metabolites have been discovered from Streptomyces, less attention has been given in understanding the bioprospecting of the secondary metabolites in Streptomyces living in mangrove ecosystem. Therefore, the current study was designed to investigate the production of actinorhodin from mangrove Streptomyces which was grown in different parameters. Phylogenetic analysis showed that the Streptomyces sp. K2-11 belongs to Streptomyces prasinus strain NRRL B-12521 with 99.62% similarity. High-performance liquid chromatography analysis has detected the presence of actinorhodin compound in Streptomyces sp. k2-11. The compound showed inhibitory activity to Bacillus subtilis and no activity against Streptococcus aureus based on disc diffusion assay. Streptomyces sp. growth in SMMS media was recorded to be the highest (1.49 × 108 cfu/ mL) compared with SYE and R2 media. The production of actinorhodin was tested on three different parameters: incubation temperature, pH and glucose levels. The highest production of actinorhodin was recorded at 33°C (1.95 × 10–6 mol/L), pH 5 (7.1 × 10–6 mol/L) and 50% w/v glucose (9.56 × 10–6 mol/L) for 60 h of incubation. The present finding suggested that mangrove Streptomyces sp. K2-11 produced high yield of actinorhodin under extreme or stress condition. 2021-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/89684/1/actinorhodin%20Azam%20full%20paper.pdf Azizan, Nur Hafizah and Sharif, Faez and Zainal Abidin, Zaima Azira and Zainuddin, Zarina and Chowdhury, Ahmed Jalal Khan (2021) Growth optimization of mangrove Streptomyces for enhancement of actinorhodin production. Desalination and Water Treatment, 219. pp. 217-222. ISSN 1944-3994/1944-3986 10.5004/dwt.2021.26841
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Azizan, Nur Hafizah
Sharif, Faez
Zainal Abidin, Zaima Azira
Zainuddin, Zarina
Chowdhury, Ahmed Jalal Khan
Growth optimization of mangrove Streptomyces for enhancement of actinorhodin production
description The family Streptomycetaceae, notably species in genus Streptomyces, have long been the subject of investigation due to their well-known ability to produce secondary metabolites. Even though thousands of secondary metabolites have been discovered from Streptomyces, less attention has been given in understanding the bioprospecting of the secondary metabolites in Streptomyces living in mangrove ecosystem. Therefore, the current study was designed to investigate the production of actinorhodin from mangrove Streptomyces which was grown in different parameters. Phylogenetic analysis showed that the Streptomyces sp. K2-11 belongs to Streptomyces prasinus strain NRRL B-12521 with 99.62% similarity. High-performance liquid chromatography analysis has detected the presence of actinorhodin compound in Streptomyces sp. k2-11. The compound showed inhibitory activity to Bacillus subtilis and no activity against Streptococcus aureus based on disc diffusion assay. Streptomyces sp. growth in SMMS media was recorded to be the highest (1.49 × 108 cfu/ mL) compared with SYE and R2 media. The production of actinorhodin was tested on three different parameters: incubation temperature, pH and glucose levels. The highest production of actinorhodin was recorded at 33°C (1.95 × 10–6 mol/L), pH 5 (7.1 × 10–6 mol/L) and 50% w/v glucose (9.56 × 10–6 mol/L) for 60 h of incubation. The present finding suggested that mangrove Streptomyces sp. K2-11 produced high yield of actinorhodin under extreme or stress condition.
format Article
author Azizan, Nur Hafizah
Sharif, Faez
Zainal Abidin, Zaima Azira
Zainuddin, Zarina
Chowdhury, Ahmed Jalal Khan
author_facet Azizan, Nur Hafizah
Sharif, Faez
Zainal Abidin, Zaima Azira
Zainuddin, Zarina
Chowdhury, Ahmed Jalal Khan
author_sort Azizan, Nur Hafizah
title Growth optimization of mangrove Streptomyces for enhancement of actinorhodin production
title_short Growth optimization of mangrove Streptomyces for enhancement of actinorhodin production
title_full Growth optimization of mangrove Streptomyces for enhancement of actinorhodin production
title_fullStr Growth optimization of mangrove Streptomyces for enhancement of actinorhodin production
title_full_unstemmed Growth optimization of mangrove Streptomyces for enhancement of actinorhodin production
title_sort growth optimization of mangrove streptomyces for enhancement of actinorhodin production
publishDate 2021
url http://irep.iium.edu.my/89684/1/actinorhodin%20Azam%20full%20paper.pdf
http://irep.iium.edu.my/89684/
_version_ 1715189375371837440
score 13.211869

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