Impedance spectroscopy of adherent mammalian cell culture for biochemical applications: a review
This review paper is focused on various applications of electric cell-substrate impedance sensing (ECIS) using adherent, two-dimensional (2D) mammalian cell culture. The ECIS technique is a non-destructive electrical approach to in vitro monitor in continuous fashion and real time the cell behavi...
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my.iium.irep.889952021-03-20T10:32:14Z http://irep.iium.edu.my/88995/ Impedance spectroscopy of adherent mammalian cell culture for biochemical applications: a review Voiculescu, Ioana R. Li, Fang Nordin, Anis Nurashikin TA Engineering (General). Civil engineering (General) TK Electrical engineering. Electronics Nuclear engineering This review paper is focused on various applications of electric cell-substrate impedance sensing (ECIS) using adherent, two-dimensional (2D) mammalian cell culture. The ECIS technique is a non-destructive electrical approach to in vitro monitor in continuous fashion and real time the cell behavior related to attachment, growth, morphology, motility, proliferation and viability. The use of living cells as sensorial elements provides the opportunity for analysis of a large variety of pharmaceutical compounds and toxicants that affect cellular responses. In this review, we will cover some of the most important applications of the ECIS technique such as: impedance of cancer cell, toxicity studies, and investigation of stem cell using impedance spectroscopy sensing. Contractile activity of beating cardiomyocytes recorded with ECIS will also be presented. Combination of impedance sensing and resonant frequency measurements of cell monolayer will be discussed. An innovative stretchable device with integrated ECIS electrodes will be discussed. Recently, the ECIS technique was combined with machine learning algorithms to distinguish stem cell proliferation from the differentiation processes. This review demonstrates that ECIS is a powerful tool, effective to investigate mammalian cell properties and physiological functions and provides advantages over conventional assays, including simple, rapid and noninvasive cell screening Institute of Electrical and Electronics Engineers Inc. 2021-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/88995/1/88995_Impedance%20Spectroscopy%20of%20Adherent_article.pdf application/pdf en http://irep.iium.edu.my/88995/2/88995_Impedance%20Spectroscopy%20of%20Adherent_scopus.pdf application/pdf en http://irep.iium.edu.my/88995/3/88995_Impedance%20Spectroscopy%20of%20Adherent_wos.pdf Voiculescu, Ioana R. and Li, Fang and Nordin, Anis Nurashikin (2021) Impedance spectroscopy of adherent mammalian cell culture for biochemical applications: a review. IEEE Sensors Journal, 21 (5). pp. 5612-5627. ISSN 1530-437X https://ieeexplore-ieee-org.ezproxy.um.edu.my/stamp/stamp.jsp?tp=&arnumber=9277877 10.1109/JSEN.2020.3041708 |
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TA Engineering (General). Civil engineering (General) TK Electrical engineering. Electronics Nuclear engineering |
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TA Engineering (General). Civil engineering (General) TK Electrical engineering. Electronics Nuclear engineering Voiculescu, Ioana R. Li, Fang Nordin, Anis Nurashikin Impedance spectroscopy of adherent mammalian cell culture for biochemical applications: a review |
| description |
This review paper is focused on various
applications of electric cell-substrate impedance sensing (ECIS) using adherent, two-dimensional (2D) mammalian
cell culture. The ECIS technique is a non-destructive electrical approach to in vitro monitor in continuous fashion and
real time the cell behavior related to attachment, growth,
morphology, motility, proliferation and viability. The use of
living cells as sensorial elements provides the opportunity for
analysis of a large variety of pharmaceutical compounds and
toxicants that affect cellular responses. In this review, we will
cover some of the most important applications of the ECIS
technique such as: impedance of cancer cell, toxicity studies,
and investigation of stem cell using impedance spectroscopy
sensing. Contractile activity of beating cardiomyocytes recorded with ECIS will also be presented. Combination of
impedance sensing and resonant frequency measurements of cell monolayer will be discussed. An innovative stretchable
device with integrated ECIS electrodes will be discussed. Recently, the ECIS technique was combined with machine
learning algorithms to distinguish stem cell proliferation from the differentiation processes. This review demonstrates
that ECIS is a powerful tool, effective to investigate mammalian cell properties and physiological functions and provides
advantages over conventional assays, including simple, rapid and noninvasive cell screening |
| format |
Article |
| author |
Voiculescu, Ioana R. Li, Fang Nordin, Anis Nurashikin |
| author_facet |
Voiculescu, Ioana R. Li, Fang Nordin, Anis Nurashikin |
| author_sort |
Voiculescu, Ioana R. |
| title |
Impedance spectroscopy of adherent mammalian cell culture for biochemical applications: a review |
| title_short |
Impedance spectroscopy of adherent mammalian cell culture for biochemical applications: a review |
| title_full |
Impedance spectroscopy of adherent mammalian cell culture for biochemical applications: a review |
| title_fullStr |
Impedance spectroscopy of adherent mammalian cell culture for biochemical applications: a review |
| title_full_unstemmed |
Impedance spectroscopy of adherent mammalian cell culture for biochemical applications: a review |
| title_sort |
impedance spectroscopy of adherent mammalian cell culture for biochemical applications: a review |
| publisher |
Institute of Electrical and Electronics Engineers Inc. |
| publishDate |
2021 |
| url |
http://irep.iium.edu.my/88995/1/88995_Impedance%20Spectroscopy%20of%20Adherent_article.pdf http://irep.iium.edu.my/88995/2/88995_Impedance%20Spectroscopy%20of%20Adherent_scopus.pdf http://irep.iium.edu.my/88995/3/88995_Impedance%20Spectroscopy%20of%20Adherent_wos.pdf http://irep.iium.edu.my/88995/ https://ieeexplore-ieee-org.ezproxy.um.edu.my/stamp/stamp.jsp?tp=&arnumber=9277877 |
| _version_ |
1695530671337897984 |
| score |
13.211869 |