Inhibition of cystathionine-γ-lyase-mediated hydrogen sulfide production in LPS-stimulated RAW 264.7 macrophages by polyherbal extract
The polyherbal formulation chandraprabha vati (CV) comprising 29 herbs is traditionally used as an anti-inflammatory agent for arthritis and urinary ailments in Indian Siddha medicine. Earlier, we reported that lipopolysaccharide (LPS) induced apoptosis in RAW 264.7 macrophages is mediated by hydr...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
NISCAIR Pubications
2021
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/88179/1/IJEB%2059%282%29%20111-117.pdf http://irep.iium.edu.my/88179/ http://nopr.niscair.res.in/bitstream/123456789/56022/1/IJEB%2059%282%29%20111-117.pdf |
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| Summary: | The polyherbal formulation chandraprabha vati (CV) comprising 29 herbs is traditionally used as an anti-inflammatory
agent for arthritis and urinary ailments in Indian Siddha medicine. Earlier, we reported that lipopolysaccharide (LPS)
induced apoptosis in RAW 264.7 macrophages is mediated by hydrogen sulfide (H2S), a potential target for many
inflammatory diseases. Here, we hypothesized that pretreatment with CV decreases H2S level and thereby alleviate the
inflammatory conditions induced by LPS in RAW 264.7 macrophages, and this protective effect occurs through alterations
in cystathionine-γ-lyase (CSE), a H2S synthesizing enzyme. Accordingly, we evaluated the anti-inflammatory effect of CV
in LPS-stimulated RAW 264.7 macrophages in vitro. The CV pretreatment followed by 12 h of LPS-stimulation showed
significantly decreased TNF-α, H2S production possibly through CSE gene expression and NF-кB activation compared to
the non pretreated macrophages. Our results further confirm |
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