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General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate

Western blotting also known as immunoblotting is a technique which was widely used in the detection of proteins. The protein of interest can be derived from various biological products. Western blotting receives its name due to the popular Southern blot (for DNA detection). The specificity of this t...

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Main Authors:	Mohd Nasir, Mohd Hamzah, Kamaruzzaman, Puteri Amirah Adib, Othman, Mohd Saiful, Muhamad Azdhar, Muhamad Shahrizar
Format:	Copyright
Language:	English English
Published:	2019
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/87553/1/general%20techniques%20in%20Western%20blotting-%20CR1%20%281%29.pdf http://irep.iium.edu.my/87553/2/WesternBlot-Manual-FinalVersion.pdf http://irep.iium.edu.my/87553/
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spelling	my.iium.irep.875532021-03-09T05:27:57Z http://irep.iium.edu.my/87553/ General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate Mohd Nasir, Mohd Hamzah Kamaruzzaman, Puteri Amirah Adib Othman, Mohd Saiful Muhamad Azdhar, Muhamad Shahrizar Q Science (General) Western blotting also known as immunoblotting is a technique which was widely used in the detection of proteins. The protein of interest can be derived from various biological products. Western blotting receives its name due to the popular Southern blot (for DNA detection). The specificity of this technique is mainly dictated by the set of antibodies used for each western blotting experiment. The precise binding between an antibody and its target protein epitope allows detection of highly specific amino acid sequence within a protein. Thus, proper experimental design and material selection is a must prior to the experiment. 2019 Copyright NonPeerReviewed application/pdf en http://irep.iium.edu.my/87553/1/general%20techniques%20in%20Western%20blotting-%20CR1%20%281%29.pdf application/pdf en http://irep.iium.edu.my/87553/2/WesternBlot-Manual-FinalVersion.pdf Mohd Nasir, Mohd Hamzah and Kamaruzzaman, Puteri Amirah Adib and Othman, Mohd Saiful and Muhamad Azdhar, Muhamad Shahrizar (2019) General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate. [Copyright] (Unpublished)
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Mohd Nasir, Mohd Hamzah Kamaruzzaman, Puteri Amirah Adib Othman, Mohd Saiful Muhamad Azdhar, Muhamad Shahrizar General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate
description	Western blotting also known as immunoblotting is a technique which was widely used in the detection of proteins. The protein of interest can be derived from various biological products. Western blotting receives its name due to the popular Southern blot (for DNA detection). The specificity of this technique is mainly dictated by the set of antibodies used for each western blotting experiment. The precise binding between an antibody and its target protein epitope allows detection of highly specific amino acid sequence within a protein. Thus, proper experimental design and material selection is a must prior to the experiment.
format	Copyright
author	Mohd Nasir, Mohd Hamzah Kamaruzzaman, Puteri Amirah Adib Othman, Mohd Saiful Muhamad Azdhar, Muhamad Shahrizar
author_facet	Mohd Nasir, Mohd Hamzah Kamaruzzaman, Puteri Amirah Adib Othman, Mohd Saiful Muhamad Azdhar, Muhamad Shahrizar
author_sort	Mohd Nasir, Mohd Hamzah
title	General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate
title_short	General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate
title_full	General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate
title_fullStr	General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate
title_full_unstemmed	General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate
title_sort	general techniques in western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate
publishDate	2019
url	http://irep.iium.edu.my/87553/1/general%20techniques%20in%20Western%20blotting-%20CR1%20%281%29.pdf http://irep.iium.edu.my/87553/2/WesternBlot-Manual-FinalVersion.pdf http://irep.iium.edu.my/87553/
_version_	1695530648608964608
score	13.211869

General techniques in Western blotting: towards effective and accurate immunoblot detection of proteins from cell lysate

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