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Chlorella vulgaris ameliorates oxidative stress and improves the muscle regenerative capacity of young and old Sprague-Dawley rats

Muscle atrophy in ageing is a multifactorial degenerative process impacted by cellular ageing biology, which includes oxidative stress. Chlorella vulgaris is a coccoid green eukaryotic microalga rich in antioxidants. The aim of this study was to determine the effect of C. vulgaris in ameliorating ox...

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Main Authors: Zainul Azlan, Nurhazirah, Mohd Yusof, Yasmin Anum, Makpol, Suzana
Format: Article
Language:English
English
English
Published: MDPI 2020
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/86571/7/86571_Chlorella%20vulgaris%20ameliorates%20oxidative%20stress%20and%20improves%20the%20muscle%20regenerative%20capacity%20of%20young%20and%20old%20sprague-dawley%20rats.pdf
http://irep.iium.edu.my/86571/13/86571_Chlorella%20vulgaris%20ameliorates%20oxidative%20stress_scopus.pdf
http://irep.iium.edu.my/86571/18/86571_Chlorella%20vulgaris%20ameliorates%20oxidative%20stress%20and%20improves%20the%20muscle_WOS.pdf
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spelling my.iium.irep.865712021-02-03T08:21:49Z http://irep.iium.edu.my/86571/ Chlorella vulgaris ameliorates oxidative stress and improves the muscle regenerative capacity of young and old Sprague-Dawley rats Zainul Azlan, Nurhazirah Mohd Yusof, Yasmin Anum Makpol, Suzana QD Chemistry Muscle atrophy in ageing is a multifactorial degenerative process impacted by cellular ageing biology, which includes oxidative stress. Chlorella vulgaris is a coccoid green eukaryotic microalga rich in antioxidants. The aim of this study was to determine the effect of C. vulgaris in ameliorating oxidative stress, thus elucidating its mechanism in improving muscle mass, strength and function in young and old rats. Fifty-six male Sprague-Dawley (SD) rats aged 3 months (young) and 21 months (old) were divided into three groups: Group 1 (control) was given distilled water; Group 2 was treated with 150 mg/kg body weight (BW) of C. vulgaris; and Group 3 was treated with 300 mg/kg BW of C. vulgaris for three months. Grip and muscle strength and muscle integrity were determined on days 0, 30, 60, and 90 of treatment. Urine and blood were collected on days 0 and 90 of treatment for oxidative stress marker determination, while the gastrocnemius muscles were collected for muscle oxidative stress analysis. Increased grip strength of the front and hind paws was observed in young C. vulgaris-treated rats on days 30, 60, and 90 compared to the untreated control on the same days (p < 0.05). There was a significant increase in lean bone mineral content (BMC) in young rats treated with 300 mg/kg BW C. vulgaris compared to untreated rats on days 30 and 60. The fat mass was significantly decreased in young and old C. vulgaris-treated rats on day 90 compared to the untreated control. The total path was significantly increased for old rats treated with 300 mg/kg BW C. vulgaris on days 60 and 90 compared to day 0. Young and old C. vulgaris-treated rats demonstrated a significant decrease in urinary isoprostane F2t and plasma creatine kinase-MM (CKMM) compared to the control on day 90. A significant decrease in malondialdehyde (MDA) and 4-hydroxyalkenal (HAE) levels were observed in young and old rats treated with C. vulgaris. C. vulgaris improved the muscle mass, strength, and function in young and old rats. This effect could be due to its potency in ameliorating oxidative stress in the skeletal muscle of young and old rats. MDPI 2020-12-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/86571/7/86571_Chlorella%20vulgaris%20ameliorates%20oxidative%20stress%20and%20improves%20the%20muscle%20regenerative%20capacity%20of%20young%20and%20old%20sprague-dawley%20rats.pdf application/pdf en http://irep.iium.edu.my/86571/13/86571_Chlorella%20vulgaris%20ameliorates%20oxidative%20stress_scopus.pdf application/pdf en http://irep.iium.edu.my/86571/18/86571_Chlorella%20vulgaris%20ameliorates%20oxidative%20stress%20and%20improves%20the%20muscle_WOS.pdf Zainul Azlan, Nurhazirah and Mohd Yusof, Yasmin Anum and Makpol, Suzana (2020) Chlorella vulgaris ameliorates oxidative stress and improves the muscle regenerative capacity of young and old Sprague-Dawley rats. Nutrients, 12 (12). pp. 1-22. ISSN 2072-6643 https://www.mdpi.com/2072-6643/12/12/3752/htm 10.3390/nu12123752
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic QD Chemistry
spellingShingle QD Chemistry
Zainul Azlan, Nurhazirah
Mohd Yusof, Yasmin Anum
Makpol, Suzana
Chlorella vulgaris ameliorates oxidative stress and improves the muscle regenerative capacity of young and old Sprague-Dawley rats
description Muscle atrophy in ageing is a multifactorial degenerative process impacted by cellular ageing biology, which includes oxidative stress. Chlorella vulgaris is a coccoid green eukaryotic microalga rich in antioxidants. The aim of this study was to determine the effect of C. vulgaris in ameliorating oxidative stress, thus elucidating its mechanism in improving muscle mass, strength and function in young and old rats. Fifty-six male Sprague-Dawley (SD) rats aged 3 months (young) and 21 months (old) were divided into three groups: Group 1 (control) was given distilled water; Group 2 was treated with 150 mg/kg body weight (BW) of C. vulgaris; and Group 3 was treated with 300 mg/kg BW of C. vulgaris for three months. Grip and muscle strength and muscle integrity were determined on days 0, 30, 60, and 90 of treatment. Urine and blood were collected on days 0 and 90 of treatment for oxidative stress marker determination, while the gastrocnemius muscles were collected for muscle oxidative stress analysis. Increased grip strength of the front and hind paws was observed in young C. vulgaris-treated rats on days 30, 60, and 90 compared to the untreated control on the same days (p < 0.05). There was a significant increase in lean bone mineral content (BMC) in young rats treated with 300 mg/kg BW C. vulgaris compared to untreated rats on days 30 and 60. The fat mass was significantly decreased in young and old C. vulgaris-treated rats on day 90 compared to the untreated control. The total path was significantly increased for old rats treated with 300 mg/kg BW C. vulgaris on days 60 and 90 compared to day 0. Young and old C. vulgaris-treated rats demonstrated a significant decrease in urinary isoprostane F2t and plasma creatine kinase-MM (CKMM) compared to the control on day 90. A significant decrease in malondialdehyde (MDA) and 4-hydroxyalkenal (HAE) levels were observed in young and old rats treated with C. vulgaris. C. vulgaris improved the muscle mass, strength, and function in young and old rats. This effect could be due to its potency in ameliorating oxidative stress in the skeletal muscle of young and old rats.
format Article
author Zainul Azlan, Nurhazirah
Mohd Yusof, Yasmin Anum
Makpol, Suzana
author_facet Zainul Azlan, Nurhazirah
Mohd Yusof, Yasmin Anum
Makpol, Suzana
author_sort Zainul Azlan, Nurhazirah
title Chlorella vulgaris ameliorates oxidative stress and improves the muscle regenerative capacity of young and old Sprague-Dawley rats
title_short Chlorella vulgaris ameliorates oxidative stress and improves the muscle regenerative capacity of young and old Sprague-Dawley rats
title_full Chlorella vulgaris ameliorates oxidative stress and improves the muscle regenerative capacity of young and old Sprague-Dawley rats
title_fullStr Chlorella vulgaris ameliorates oxidative stress and improves the muscle regenerative capacity of young and old Sprague-Dawley rats
title_full_unstemmed Chlorella vulgaris ameliorates oxidative stress and improves the muscle regenerative capacity of young and old Sprague-Dawley rats
title_sort chlorella vulgaris ameliorates oxidative stress and improves the muscle regenerative capacity of young and old sprague-dawley rats
publisher MDPI
publishDate 2020
url http://irep.iium.edu.my/86571/7/86571_Chlorella%20vulgaris%20ameliorates%20oxidative%20stress%20and%20improves%20the%20muscle%20regenerative%20capacity%20of%20young%20and%20old%20sprague-dawley%20rats.pdf
http://irep.iium.edu.my/86571/13/86571_Chlorella%20vulgaris%20ameliorates%20oxidative%20stress_scopus.pdf
http://irep.iium.edu.my/86571/18/86571_Chlorella%20vulgaris%20ameliorates%20oxidative%20stress%20and%20improves%20the%20muscle_WOS.pdf
http://irep.iium.edu.my/86571/
https://www.mdpi.com/2072-6643/12/12/3752/htm
_version_ 1691732913094131712
score 13.211869

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