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Purification and Cytotoxicity Assay of TOmato (Lycopersicum esculentum) Leaves Methanol Extract as Potential Anticancer Agent

This research studied the cytotoxicity effect of tomato leaves methanol extract on cancer cells to address potential therapeutic in MCF-7 breast cancer cell lines and its toxicity towards Vero cells. The extraction was done in a shake flask by 82% methanol, 1:10 (w/v), agitated at 22°C with 110 rpm...

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Main Authors: Wan Chik, Wan Dalila, Amid, Azura, Jamal, Parveen
Format: Article
Language:English
Published: Asian Network for Scientific Information 2010
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/862/1/Anti_cancer_tomato_leaves.pdf
http://irep.iium.edu.my/862/
http://scialert.net/abstract/?doi=jas.2010.3283.3288
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spelling my.iium.irep.8622011-12-20T01:37:30Z http://irep.iium.edu.my/862/ Purification and Cytotoxicity Assay of TOmato (Lycopersicum esculentum) Leaves Methanol Extract as Potential Anticancer Agent Wan Chik, Wan Dalila Amid, Azura Jamal, Parveen TP248.13 Biotechnology This research studied the cytotoxicity effect of tomato leaves methanol extract on cancer cells to address potential therapeutic in MCF-7 breast cancer cell lines and its toxicity towards Vero cells. The extraction was done in a shake flask by 82% methanol, 1:10 (w/v), agitated at 22°C with 110 rpm within 24 h. Later, purification process was started by Thin Layer Chromatography (TLC) subjected to determine the best mobile phase for compound separation and collection by means of column chromatography. Next, the effect of purified sample towards MCF-7 breast cancer cell lines and Vero cells were observed using in vitro cytotoxicity assay to indicate its active fractions and its half maximal inhibitory concentration (IC50). Purified sample gave a rational effect towards MCF-7 breast cancer cells with IC50 value of 5.85 μg mL-1 compared to Taxol with IC50 value of 0.039 μg mL-1. The purified sample can also be judged to be harmless as it has IC50 value of 765.6 μg mL-1 in Vero cells treatment while Taxol gave IC50 value of 0.045 μg mL-1. Asian Network for Scientific Information 2010 Article REM application/pdf en http://irep.iium.edu.my/862/1/Anti_cancer_tomato_leaves.pdf Wan Chik, Wan Dalila and Amid, Azura and Jamal, Parveen (2010) Purification and Cytotoxicity Assay of TOmato (Lycopersicum esculentum) Leaves Methanol Extract as Potential Anticancer Agent. Journal of Applied Sciences, 10 (24). pp. 3283-3288. ISSN 1812-5662 (O), 1812-5654 (P) http://scialert.net/abstract/?doi=jas.2010.3283.3288 DOI: 10.3923/jas.2010.3283.3288
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Wan Chik, Wan Dalila
Amid, Azura
Jamal, Parveen
Purification and Cytotoxicity Assay of TOmato (Lycopersicum esculentum) Leaves Methanol Extract as Potential Anticancer Agent
description This research studied the cytotoxicity effect of tomato leaves methanol extract on cancer cells to address potential therapeutic in MCF-7 breast cancer cell lines and its toxicity towards Vero cells. The extraction was done in a shake flask by 82% methanol, 1:10 (w/v), agitated at 22°C with 110 rpm within 24 h. Later, purification process was started by Thin Layer Chromatography (TLC) subjected to determine the best mobile phase for compound separation and collection by means of column chromatography. Next, the effect of purified sample towards MCF-7 breast cancer cell lines and Vero cells were observed using in vitro cytotoxicity assay to indicate its active fractions and its half maximal inhibitory concentration (IC50). Purified sample gave a rational effect towards MCF-7 breast cancer cells with IC50 value of 5.85 μg mL-1 compared to Taxol with IC50 value of 0.039 μg mL-1. The purified sample can also be judged to be harmless as it has IC50 value of 765.6 μg mL-1 in Vero cells treatment while Taxol gave IC50 value of 0.045 μg mL-1.
format Article
author Wan Chik, Wan Dalila
Amid, Azura
Jamal, Parveen
author_facet Wan Chik, Wan Dalila
Amid, Azura
Jamal, Parveen
author_sort Wan Chik, Wan Dalila
title Purification and Cytotoxicity Assay of TOmato (Lycopersicum esculentum) Leaves Methanol Extract as Potential Anticancer Agent
title_short Purification and Cytotoxicity Assay of TOmato (Lycopersicum esculentum) Leaves Methanol Extract as Potential Anticancer Agent
title_full Purification and Cytotoxicity Assay of TOmato (Lycopersicum esculentum) Leaves Methanol Extract as Potential Anticancer Agent
title_fullStr Purification and Cytotoxicity Assay of TOmato (Lycopersicum esculentum) Leaves Methanol Extract as Potential Anticancer Agent
title_full_unstemmed Purification and Cytotoxicity Assay of TOmato (Lycopersicum esculentum) Leaves Methanol Extract as Potential Anticancer Agent
title_sort purification and cytotoxicity assay of tomato (lycopersicum esculentum) leaves methanol extract as potential anticancer agent
publisher Asian Network for Scientific Information
publishDate 2010
url http://irep.iium.edu.my/862/1/Anti_cancer_tomato_leaves.pdf
http://irep.iium.edu.my/862/
http://scialert.net/abstract/?doi=jas.2010.3283.3288
_version_ 1643604687020097536
score 13.211869

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