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Effect of phenotypic switching on the susceptibility of candida krusei towards amphotericin B, nystatin and piper betle aqueous extract

Candida krusei (C. krusei) is associated with oral candidiasis, particularly in immune-compromised patients. The objective of the study was to determine the effect of phenotypic switching to the susceptibility of C. krusei towards amphotericin B, nystatin and aqueous extract of Piper betle (P. betle...

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Main Authors: Arzmi, Mohd Hafiz, Abdul Razak, Fathilah, Wan Harun, Wan Himratul Aznita, Wan Mohd Kamaluddin, Wan Nur Fatihah, Rismayuddin, Nurul Alia Risma
Format: Article
Language:English
Published: IIUM Press 2020
Subjects:
QR Microbiology
RK Dentistry
Online Access:http://irep.iium.edu.my/86011/13/86011_Effect%20of%20phenotypic%20switching%20on%20the%20susceptibility.pdf
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spelling my.iium.irep.860112020-12-15T19:31:48Z http://irep.iium.edu.my/86011/ Effect of phenotypic switching on the susceptibility of candida krusei towards amphotericin B, nystatin and piper betle aqueous extract Arzmi, Mohd Hafiz Abdul Razak, Fathilah Wan Harun, Wan Himratul Aznita Wan Mohd Kamaluddin, Wan Nur Fatihah Rismayuddin, Nurul Alia Risma QR Microbiology RK Dentistry Candida krusei (C. krusei) is associated with oral candidiasis, particularly in immune-compromised patients. The objective of the study was to determine the effect of phenotypic switching to the susceptibility of C. krusei towards amphotericin B, nystatin and aqueous extract of Piper betle (P. betle). To induce phenotypic switching, C. krusei was inoculated in yeast extract peptone dextrose (YEPD) broth supplemented with 5 mg mL-1 phloxine B and incubated for five hours at 25 C. Later, 100 μL of the suspension was inoculated on YEPD agar supplemented with 5 mg mL-1 phloxine B and incubated for five days at 25 C. Disc diffusion and minimum inhibitory concentration (MIC) assays were conducted to determine the susceptibility of C. krusei. The results showed that all C. krusei switched generations were susceptible towards amphotericin B and nystatin with the 3rd and 4th generations significantly more susceptible than the un-switched, respectively (P<0.05). All C. krusei switched generations were also observed to be susceptible towards the aqueous extract of P. betle. The MIC of amphotericin B, nystatin and P. betle were determined at 10 μg mL-1, 10 μg mL-1 and 12.5 mg mL-1, respectively for all generations of C. krusei. In conclusion, the susceptibility of C. krusei was phenotypically switched generation dependent towards amphotericin B, nystatin, and Piper betle aqueous extract. IIUM Press 2020 Article PeerReviewed application/pdf en http://irep.iium.edu.my/86011/13/86011_Effect%20of%20phenotypic%20switching%20on%20the%20susceptibility.pdf Arzmi, Mohd Hafiz and Abdul Razak, Fathilah and Wan Harun, Wan Himratul Aznita and Wan Mohd Kamaluddin, Wan Nur Fatihah and Rismayuddin, Nurul Alia Risma (2020) Effect of phenotypic switching on the susceptibility of candida krusei towards amphotericin B, nystatin and piper betle aqueous extract. IIUM Journal of Orofacial and Health Sciences, 1 (2). pp. 69-77. E-ISSN 2735-0584 https://journals.iium.edu.my/ktn/index.php/ijohs/about
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QR Microbiology
RK Dentistry
spellingShingle QR Microbiology
RK Dentistry
Arzmi, Mohd Hafiz
Abdul Razak, Fathilah
Wan Harun, Wan Himratul Aznita
Wan Mohd Kamaluddin, Wan Nur Fatihah
Rismayuddin, Nurul Alia Risma
Effect of phenotypic switching on the susceptibility of candida krusei towards amphotericin B, nystatin and piper betle aqueous extract
description Candida krusei (C. krusei) is associated with oral candidiasis, particularly in immune-compromised patients. The objective of the study was to determine the effect of phenotypic switching to the susceptibility of C. krusei towards amphotericin B, nystatin and aqueous extract of Piper betle (P. betle). To induce phenotypic switching, C. krusei was inoculated in yeast extract peptone dextrose (YEPD) broth supplemented with 5 mg mL-1 phloxine B and incubated for five hours at 25 C. Later, 100 μL of the suspension was inoculated on YEPD agar supplemented with 5 mg mL-1 phloxine B and incubated for five days at 25 C. Disc diffusion and minimum inhibitory concentration (MIC) assays were conducted to determine the susceptibility of C. krusei. The results showed that all C. krusei switched generations were susceptible towards amphotericin B and nystatin with the 3rd and 4th generations significantly more susceptible than the un-switched, respectively (P<0.05). All C. krusei switched generations were also observed to be susceptible towards the aqueous extract of P. betle. The MIC of amphotericin B, nystatin and P. betle were determined at 10 μg mL-1, 10 μg mL-1 and 12.5 mg mL-1, respectively for all generations of C. krusei. In conclusion, the susceptibility of C. krusei was phenotypically switched generation dependent towards amphotericin B, nystatin, and Piper betle aqueous extract.
format Article
author Arzmi, Mohd Hafiz
Abdul Razak, Fathilah
Wan Harun, Wan Himratul Aznita
Wan Mohd Kamaluddin, Wan Nur Fatihah
Rismayuddin, Nurul Alia Risma
author_facet Arzmi, Mohd Hafiz
Abdul Razak, Fathilah
Wan Harun, Wan Himratul Aznita
Wan Mohd Kamaluddin, Wan Nur Fatihah
Rismayuddin, Nurul Alia Risma
author_sort Arzmi, Mohd Hafiz
title Effect of phenotypic switching on the susceptibility of candida krusei towards amphotericin B, nystatin and piper betle aqueous extract
title_short Effect of phenotypic switching on the susceptibility of candida krusei towards amphotericin B, nystatin and piper betle aqueous extract
title_full Effect of phenotypic switching on the susceptibility of candida krusei towards amphotericin B, nystatin and piper betle aqueous extract
title_fullStr Effect of phenotypic switching on the susceptibility of candida krusei towards amphotericin B, nystatin and piper betle aqueous extract
title_full_unstemmed Effect of phenotypic switching on the susceptibility of candida krusei towards amphotericin B, nystatin and piper betle aqueous extract
title_sort effect of phenotypic switching on the susceptibility of candida krusei towards amphotericin b, nystatin and piper betle aqueous extract
publisher IIUM Press
publishDate 2020
url http://irep.iium.edu.my/86011/13/86011_Effect%20of%20phenotypic%20switching%20on%20the%20susceptibility.pdf
http://irep.iium.edu.my/86011/
https://journals.iium.edu.my/ktn/index.php/ijohs/about
_version_ 1687393127317372928
score 13.211869

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