Anti-inflammatory and analgesic effects of trachyspermum ammi seed extract and Its GC-MS analysis
Introduction: Since ancient times Trachyspermum ammi was utilized for its anti-inflammatory and analgesic effects in traditional medicine system. Objectives: Present study aimed to evaluate the anti-inflammatory and analgesic effect and characterization of chemical composition seed extract of T...
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my.iium.irep.858742020-12-11T00:47:10Z http://irep.iium.edu.my/85874/ Anti-inflammatory and analgesic effects of trachyspermum ammi seed extract and Its GC-MS analysis Aslam, Adeel Nokhala, Ahmed Peerzada, Sohaib Ahmed, Shoaib Khan, Tanveer Siddiqui, Mohammad Jamshed Ahmad QD Chemistry Introduction: Since ancient times Trachyspermum ammi was utilized for its anti-inflammatory and analgesic effects in traditional medicine system. Objectives: Present study aimed to evaluate the anti-inflammatory and analgesic effect and characterization of chemical composition seed extract of T. ammi through GC-MS. Material and Methods: Seed extracts were prepared through soxhlet extraction using n-hexane, chloroform and methanol as solvents. Gas chromatography-mass spectroscopy (GCMS) analysis was performed by derivatized sample of T. ammi extract. Acute toxicity test performed at dose of 400mg/kg, 800mg/kg, 1600mg/kg and 3200mg/kg. Two different strengths (minimum therapeutic dose 500mg/ kg and maximum therapeutic dose1000mg/kg) were given to Wistar rats to measure anti-inflammatory and analgesic activity. Results: Results from analgesic and antiinflammatory activity demonstrate that in both activities standard drug (tramadol) have more analgesic (percentage of analgesia 26.08%) and regarding anti-inflammatory effect diclofenac sodium (percentage of inhibition of paw edema 29.68%) more effective as compared to test drug. When efficacy of all extracts compared with each other n-hexane extract showed more percentage of inhibition (percentage analgesia 20.69% and percentage inhibition of paw edema 22.21%) at maximum effective dose 1000mg/kg. In order to find out significance difference among groups ANOVA test was used at 95% significant level. A number of different bioactive compounds were identified through GC-MS analysis. Conclusion: Results showed that T. ammi seed extracts have anti-inflammatory and analgesic activity potentiating in neurotransmission of GABA and repression of receptor of glutamate along with suppression of nitric oxide (NO) pathway. Phenolic compounds present in seeds may responsible for anti-inflammatory activity. Medknow 2020-11-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/85874/1/JPBS_Abstract%20_ID99.pdf Aslam, Adeel and Nokhala, Ahmed and Peerzada, Sohaib and Ahmed, Shoaib and Khan, Tanveer and Siddiqui, Mohammad Jamshed Ahmad (2020) Anti-inflammatory and analgesic effects of trachyspermum ammi seed extract and Its GC-MS analysis. Journal of Pharmacy and Bioallied Sciences, 12 (Supplement 2). p. 886. ISSN 0976-4879 E-ISSN 0975-7406 http://www.jpbsonline.org |
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QD Chemistry Aslam, Adeel Nokhala, Ahmed Peerzada, Sohaib Ahmed, Shoaib Khan, Tanveer Siddiqui, Mohammad Jamshed Ahmad Anti-inflammatory and analgesic effects of trachyspermum ammi seed extract and Its GC-MS analysis |
| description |
Introduction: Since ancient times Trachyspermum ammi
was utilized for its anti-inflammatory and analgesic effects
in traditional medicine system. Objectives: Present study
aimed to evaluate the anti-inflammatory and analgesic
effect and characterization of chemical composition
seed extract of T. ammi through GC-MS. Material and
Methods: Seed extracts were prepared through soxhlet
extraction using n-hexane, chloroform and methanol as
solvents. Gas chromatography-mass spectroscopy (GCMS)
analysis was performed by derivatized sample of
T. ammi extract. Acute toxicity test performed at dose of
400mg/kg, 800mg/kg, 1600mg/kg and 3200mg/kg. Two
different strengths (minimum therapeutic dose 500mg/
kg and maximum therapeutic dose1000mg/kg) were
given to Wistar rats to measure anti-inflammatory and
analgesic activity. Results: Results from analgesic and antiinflammatory
activity demonstrate that in both activities
standard drug (tramadol) have more analgesic (percentage
of analgesia 26.08%) and regarding anti-inflammatory
effect diclofenac sodium (percentage of inhibition of paw
edema 29.68%) more effective as compared to test drug.
When efficacy of all extracts compared with each other
n-hexane extract showed more percentage of inhibition
(percentage analgesia 20.69% and percentage inhibition of
paw edema 22.21%) at maximum effective dose 1000mg/kg.
In order to find out significance difference among groups
ANOVA test was used at 95% significant level. A number
of different bioactive compounds were identified through
GC-MS analysis. Conclusion: Results showed that T. ammi
seed extracts have anti-inflammatory and analgesic activity
potentiating in neurotransmission of GABA and repression
of receptor of glutamate along with suppression of nitric
oxide (NO) pathway. Phenolic compounds present in seeds
may responsible for anti-inflammatory activity. |
| format |
Article |
| author |
Aslam, Adeel Nokhala, Ahmed Peerzada, Sohaib Ahmed, Shoaib Khan, Tanveer Siddiqui, Mohammad Jamshed Ahmad |
| author_facet |
Aslam, Adeel Nokhala, Ahmed Peerzada, Sohaib Ahmed, Shoaib Khan, Tanveer Siddiqui, Mohammad Jamshed Ahmad |
| author_sort |
Aslam, Adeel |
| title |
Anti-inflammatory and analgesic effects of trachyspermum ammi seed extract and Its GC-MS analysis |
| title_short |
Anti-inflammatory and analgesic effects of trachyspermum ammi seed extract and Its GC-MS analysis |
| title_full |
Anti-inflammatory and analgesic effects of trachyspermum ammi seed extract and Its GC-MS analysis |
| title_fullStr |
Anti-inflammatory and analgesic effects of trachyspermum ammi seed extract and Its GC-MS analysis |
| title_full_unstemmed |
Anti-inflammatory and analgesic effects of trachyspermum ammi seed extract and Its GC-MS analysis |
| title_sort |
anti-inflammatory and analgesic effects of trachyspermum ammi seed extract and its gc-ms analysis |
| publisher |
Medknow |
| publishDate |
2020 |
| url |
http://irep.iium.edu.my/85874/1/JPBS_Abstract%20_ID99.pdf http://irep.iium.edu.my/85874/ http://www.jpbsonline.org |
| _version_ |
1687393115690762240 |
| score |
13.211869 |