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Reduced graphene oxide on screen-printed carbon electrodes as biosensor for Escherichia coli O157:H7 detection

Rapid development and deployment of biosensors for pathogen quantification is needed for flood-stricken areas. Drinking-water supplies in flooded areas become a disease threat owing to mixture with sewage discharge. In such exigent conditions, water is filtered through a microfiltration technique th...

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Main Authors: Barthasarathy, Piravin Raj, Wan Salim, Wan Wardatul Amani
Format: Conference or Workshop Item
Language:English
Published: MDPI 2020
Subjects:
QR Microbiology
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/84241/1/84241_Reduced%20Graphene%20Oxide%20on%20Screen-Printed.pdf
http://irep.iium.edu.my/84241/
https://sciforum.net/paper/view/conference/7056
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spelling my.iium.irep.842412020-11-04T02:57:55Z http://irep.iium.edu.my/84241/ Reduced graphene oxide on screen-printed carbon electrodes as biosensor for Escherichia coli O157:H7 detection Barthasarathy, Piravin Raj Wan Salim, Wan Wardatul Amani QR Microbiology TP248.13 Biotechnology Rapid development and deployment of biosensors for pathogen quantification is needed for flood-stricken areas. Drinking-water supplies in flooded areas become a disease threat owing to mixture with sewage discharge. In such exigent conditions, water is filtered through a microfiltration technique that is moderately effective in removing bacteria and non-effective in removing viruses. Quantification of pathogens using biochemical techniques does not provide real-time data and requires transportation of water samples to laboratories for analysis. However, transportation services are severely affected during flooding, and hence on-site testing may be the only option. WHO specifies that diagnostic devices are to be made ASSURED – affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable to end-users. To fulfil ASSURED criteria, we developed a low-cost electrochemical biosensor integrated with an Arduino® microprocessor encased in a portable platform for E. coli O157:H7 quantification. The working electrode (WE) of a screen-printed carbon electrode (SPCE) was modified with reduced graphene oxide (rGO). Rabbit serum IgG, a non-specific antibody towards E. coli O157:H7, was immobilized on the WE. We tested the specificity of E. coli O157:H7 binding to IgG/rGO/SPCEs and rGO/SPCEs at 4 to 4 × 108 CFU/ml. We chose a potential range of -0.35 to 0.07 in LSV to compare the current values at different concentrations. At -0.14V, IgG/rGO/SPCEs distinguished E. coli concentrations of 4 and 4 × 108 CFU/ml with current value of 17 µA and 1 µA, respectively. However, the IgG/rGO/SPCE does not distinguish between E. coli concentration of 4 × 107 and 4 × 108 CFU/ml. The rGO/SPCE does not distinguish E. coli concentration of 4 and 4 × 108 CFU/ml, as evidenced by almost similar current values in both concentrations. This study indicates the potential of non-specific antibodies for E. coli O157:H7 quantification during water-quality monitoring in flooded areas. MDPI 2020-11-02 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/84241/1/84241_Reduced%20Graphene%20Oxide%20on%20Screen-Printed.pdf Barthasarathy, Piravin Raj and Wan Salim, Wan Wardatul Amani (2020) Reduced graphene oxide on screen-printed carbon electrodes as biosensor for Escherichia coli O157:H7 detection. In: The 1st International Electronic Conference on Biosensors (IECB 2020), 02nd-17th November 2020, Online platform. https://sciforum.net/paper/view/conference/7056 10.3390/IECB2020-07056
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QR Microbiology
TP248.13 Biotechnology
spellingShingle QR Microbiology
TP248.13 Biotechnology
Barthasarathy, Piravin Raj
Wan Salim, Wan Wardatul Amani
Reduced graphene oxide on screen-printed carbon electrodes as biosensor for Escherichia coli O157:H7 detection
description Rapid development and deployment of biosensors for pathogen quantification is needed for flood-stricken areas. Drinking-water supplies in flooded areas become a disease threat owing to mixture with sewage discharge. In such exigent conditions, water is filtered through a microfiltration technique that is moderately effective in removing bacteria and non-effective in removing viruses. Quantification of pathogens using biochemical techniques does not provide real-time data and requires transportation of water samples to laboratories for analysis. However, transportation services are severely affected during flooding, and hence on-site testing may be the only option. WHO specifies that diagnostic devices are to be made ASSURED – affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable to end-users. To fulfil ASSURED criteria, we developed a low-cost electrochemical biosensor integrated with an Arduino® microprocessor encased in a portable platform for E. coli O157:H7 quantification. The working electrode (WE) of a screen-printed carbon electrode (SPCE) was modified with reduced graphene oxide (rGO). Rabbit serum IgG, a non-specific antibody towards E. coli O157:H7, was immobilized on the WE. We tested the specificity of E. coli O157:H7 binding to IgG/rGO/SPCEs and rGO/SPCEs at 4 to 4 × 108 CFU/ml. We chose a potential range of -0.35 to 0.07 in LSV to compare the current values at different concentrations. At -0.14V, IgG/rGO/SPCEs distinguished E. coli concentrations of 4 and 4 × 108 CFU/ml with current value of 17 µA and 1 µA, respectively. However, the IgG/rGO/SPCE does not distinguish between E. coli concentration of 4 × 107 and 4 × 108 CFU/ml. The rGO/SPCE does not distinguish E. coli concentration of 4 and 4 × 108 CFU/ml, as evidenced by almost similar current values in both concentrations. This study indicates the potential of non-specific antibodies for E. coli O157:H7 quantification during water-quality monitoring in flooded areas.
format Conference or Workshop Item
author Barthasarathy, Piravin Raj
Wan Salim, Wan Wardatul Amani
author_facet Barthasarathy, Piravin Raj
Wan Salim, Wan Wardatul Amani
author_sort Barthasarathy, Piravin Raj
title Reduced graphene oxide on screen-printed carbon electrodes as biosensor for Escherichia coli O157:H7 detection
title_short Reduced graphene oxide on screen-printed carbon electrodes as biosensor for Escherichia coli O157:H7 detection
title_full Reduced graphene oxide on screen-printed carbon electrodes as biosensor for Escherichia coli O157:H7 detection
title_fullStr Reduced graphene oxide on screen-printed carbon electrodes as biosensor for Escherichia coli O157:H7 detection
title_full_unstemmed Reduced graphene oxide on screen-printed carbon electrodes as biosensor for Escherichia coli O157:H7 detection
title_sort reduced graphene oxide on screen-printed carbon electrodes as biosensor for escherichia coli o157:h7 detection
publisher MDPI
publishDate 2020
url http://irep.iium.edu.my/84241/1/84241_Reduced%20Graphene%20Oxide%20on%20Screen-Printed.pdf
http://irep.iium.edu.my/84241/
https://sciforum.net/paper/view/conference/7056
_version_ 1683230374526713856
score 13.211869

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