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DNA barcoding of ichthyoplankton and juvenile fishes of a tropical river in Malaysia

Taxonomic identification of early larval stages of fishes using conventional morphological keys is extremely laborious due to the overlapping characters shared between genetically closer species. Especially, species-level differentiation during their ontological development is challenging due to th...

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Main Authors: John, Akbar, Sheikh Mohamed, Hassan Ibrahim, Khan Chowdhury, Ahmed Jalal, Yunus, Kamaruzzaman, Hossain, Md. Sanower, Mohd Noor, Hanisah, Rahman, Mohammad Mustafizur, Mohamed, Rozihan
Format: Book Chapter
Language:English
Published: Springer 2020
Subjects:
SH Aquaculture. Fisheries. Angling
Online Access:http://irep.iium.edu.my/83231/1/83231_DNA%20Barcoding%20of%20Ichthyoplankton.pdf
http://irep.iium.edu.my/83231/
https://link.springer.com/chapter/10.1007/978-3-030-50075-7_15
https://doi.org/10.1007/978-3-030-50075-7_15
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spelling my.iium.irep.832312021-02-01T03:48:03Z http://irep.iium.edu.my/83231/ DNA barcoding of ichthyoplankton and juvenile fishes of a tropical river in Malaysia John, Akbar Sheikh Mohamed, Hassan Ibrahim Khan Chowdhury, Ahmed Jalal Yunus, Kamaruzzaman Hossain, Md. Sanower Mohd Noor, Hanisah Rahman, Mohammad Mustafizur Mohamed, Rozihan SH Aquaculture. Fisheries. Angling Taxonomic identification of early larval stages of fishes using conventional morphological keys is extremely laborious due to the overlapping characters shared between genetically closer species. Especially, species-level differentiation during their ontological development is challenging due to the paucity of information on their diagnostic features. In the present study, we aimed to use universal DNA barcoding technology to identify Ichthyoplankton and juvenile fishes of a tropical river (Kuantan River in Pahang) in Malaysia. This sampling station was chosen in order to check the distribution of juvenile and fish Ichthyoplankton samples after the recent massive flood encountered in East peninsular Malaysia during 2014. We adopted mitochondrial cytochrome oxidase C subunit 1 gene sequencing to identify fish samples. A total of 28 species from 15 families and 5 orders were identified successfully to the species level from the total of 58 DNA barcodes. Unlike the previous report, the most dominant fishes in this study belong to the Cyprinidae family followed by toxotidae, ambasidae, and eleotridae. We admit that the modified bubu light trap method adopted for larval collection in this study has its limitation to attract larvae which had negative phototactic behavior (i.e., Ariidae fishes). Phylogenetic and BLAST analysis showed accuracy of species identification with high bootstrap and percentage similarity value, respectively. Results in this study confirmed the efficiency of universal DNA barcode technology in species-level delimitation of morphologically cryptic species identification. The data presented in this study are valuable for analyzing post-flood effect on fish distribution in Tropical River and implementing plans for future fishery resource management in Kuantan River, Pahang, Malaysia. Springer 2020-08-25 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/83231/1/83231_DNA%20Barcoding%20of%20Ichthyoplankton.pdf John, Akbar and Sheikh Mohamed, Hassan Ibrahim and Khan Chowdhury, Ahmed Jalal and Yunus, Kamaruzzaman and Hossain, Md. Sanower and Mohd Noor, Hanisah and Rahman, Mohammad Mustafizur and Mohamed, Rozihan (2020) DNA barcoding of ichthyoplankton and juvenile fishes of a tropical river in Malaysia. In: DNA barcoding and molecular phylogeny. Springer, Switzerland, pp. 237-251. ISBN 978-3-030-50074-0 https://link.springer.com/chapter/10.1007/978-3-030-50075-7_15 https://doi.org/10.1007/978-3-030-50075-7_15
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic SH Aquaculture. Fisheries. Angling
spellingShingle SH Aquaculture. Fisheries. Angling
John, Akbar
Sheikh Mohamed, Hassan Ibrahim
Khan Chowdhury, Ahmed Jalal
Yunus, Kamaruzzaman
Hossain, Md. Sanower
Mohd Noor, Hanisah
Rahman, Mohammad Mustafizur
Mohamed, Rozihan
DNA barcoding of ichthyoplankton and juvenile fishes of a tropical river in Malaysia
description Taxonomic identification of early larval stages of fishes using conventional morphological keys is extremely laborious due to the overlapping characters shared between genetically closer species. Especially, species-level differentiation during their ontological development is challenging due to the paucity of information on their diagnostic features. In the present study, we aimed to use universal DNA barcoding technology to identify Ichthyoplankton and juvenile fishes of a tropical river (Kuantan River in Pahang) in Malaysia. This sampling station was chosen in order to check the distribution of juvenile and fish Ichthyoplankton samples after the recent massive flood encountered in East peninsular Malaysia during 2014. We adopted mitochondrial cytochrome oxidase C subunit 1 gene sequencing to identify fish samples. A total of 28 species from 15 families and 5 orders were identified successfully to the species level from the total of 58 DNA barcodes. Unlike the previous report, the most dominant fishes in this study belong to the Cyprinidae family followed by toxotidae, ambasidae, and eleotridae. We admit that the modified bubu light trap method adopted for larval collection in this study has its limitation to attract larvae which had negative phototactic behavior (i.e., Ariidae fishes). Phylogenetic and BLAST analysis showed accuracy of species identification with high bootstrap and percentage similarity value, respectively. Results in this study confirmed the efficiency of universal DNA barcode technology in species-level delimitation of morphologically cryptic species identification. The data presented in this study are valuable for analyzing post-flood effect on fish distribution in Tropical River and implementing plans for future fishery resource management in Kuantan River, Pahang, Malaysia.
format Book Chapter
author John, Akbar
Sheikh Mohamed, Hassan Ibrahim
Khan Chowdhury, Ahmed Jalal
Yunus, Kamaruzzaman
Hossain, Md. Sanower
Mohd Noor, Hanisah
Rahman, Mohammad Mustafizur
Mohamed, Rozihan
author_facet John, Akbar
Sheikh Mohamed, Hassan Ibrahim
Khan Chowdhury, Ahmed Jalal
Yunus, Kamaruzzaman
Hossain, Md. Sanower
Mohd Noor, Hanisah
Rahman, Mohammad Mustafizur
Mohamed, Rozihan
author_sort John, Akbar
title DNA barcoding of ichthyoplankton and juvenile fishes of a tropical river in Malaysia
title_short DNA barcoding of ichthyoplankton and juvenile fishes of a tropical river in Malaysia
title_full DNA barcoding of ichthyoplankton and juvenile fishes of a tropical river in Malaysia
title_fullStr DNA barcoding of ichthyoplankton and juvenile fishes of a tropical river in Malaysia
title_full_unstemmed DNA barcoding of ichthyoplankton and juvenile fishes of a tropical river in Malaysia
title_sort dna barcoding of ichthyoplankton and juvenile fishes of a tropical river in malaysia
publisher Springer
publishDate 2020
url http://irep.iium.edu.my/83231/1/83231_DNA%20Barcoding%20of%20Ichthyoplankton.pdf
http://irep.iium.edu.my/83231/
https://link.springer.com/chapter/10.1007/978-3-030-50075-7_15
https://doi.org/10.1007/978-3-030-50075-7_15
_version_ 1691732909321355264
score 13.222552

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