Staff View: Lipid accumulation modulation by garcinia atroviridis fruit extract in 3T3-L1 adipocyte cells

Lipid accumulation modulation by garcinia atroviridis fruit extract in 3T3-L1 adipocyte cells

Garcinia atroviridis contains hydroxycitric acid (HCA) that affects obesity and obesityrelated diseases. This paper aims to quantify the amount of (HCA) available in the fruit extract of Garcinia atroviridis and investigate the lipid modulation properties possessed by the extract in the 3T3-L1 adip...

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Main Authors:	Darnis, Deny Susanti, Hamidon, Hanisuhana, Bakhtiar, M. Taher, Ahmad Rusmili, Muhamad Rusdi, Abd Hamid, Shafida, Zakaria, Zainul Amiruddin
Format:	Article
Language:	English
Published:	Taylor & Francis Online 2020
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/82840/1/Lipid%20Accumulation%20Modulation%20by%20Garcin...3-L1%20Adipocyte%20Cells%20_%20Enhanced%20Reader.pdf http://irep.iium.edu.my/82840/ https://www.tandfonline.com/doi/abs/10.1080/22311866.2020.1804448
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spelling	my.iium.irep.828402020-10-27T03:36:51Z http://irep.iium.edu.my/82840/ Lipid accumulation modulation by garcinia atroviridis fruit extract in 3T3-L1 adipocyte cells Darnis, Deny Susanti Hamidon, Hanisuhana Bakhtiar, M. Taher Ahmad Rusmili, Muhamad Rusdi Abd Hamid, Shafida Zakaria, Zainul Amiruddin RS Pharmacy and materia medica Garcinia atroviridis contains hydroxycitric acid (HCA) that affects obesity and obesityrelated diseases. This paper aims to quantify the amount of (HCA) available in the fruit extract of Garcinia atroviridis and investigate the lipid modulation properties possessed by the extract in the 3T3-L1 adipocyte cell line. High-performance liquid chromatography (HPLC) analysis was used to quantify HCA in the extract, whereas differentiated adipocytes were evaluated Oil Red O staining. The expression of PPARγ and C/EBPα was investigated using western blot, and the lipid content was confirmed using leptin ELISA assay. HPLC analysis quantified about 11.35 ± 0.55 % (w/w) of HCA in the fruit extract, with a retention time of 0.9 minutes corresponding to the standard. In the adipogenesis assay, an increased co-treatment concentration was observed and inhibited the formation of intracellular lipids. According to the western blot analysis, the treatment was found to significantly affect the expression of C/EBPα proteins solely. Meanwhile, the leptin assay finding was in agreement with the adipogenesis analysis as a decrement of leptin released was noted at increased treatment concentrations. Finally, the fruit extract enhanced the adipocyte breakdown (adipolysis) process as revealed by the glycerol concentration measured in the adipolysis assay. The findings indicated that the fruit extract of G. atroviridis played a role in the alteration of lipid accumulation processes by the intervention of proteins associated with adipogenesis and enhanced adipolysis. Taylor & Francis Online 2020-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82840/1/Lipid%20Accumulation%20Modulation%20by%20Garcin...3-L1%20Adipocyte%20Cells%20_%20Enhanced%20Reader.pdf Darnis, Deny Susanti and Hamidon, Hanisuhana and Bakhtiar, M. Taher and Ahmad Rusmili, Muhamad Rusdi and Abd Hamid, Shafida and Zakaria, Zainul Amiruddin (2020) Lipid accumulation modulation by garcinia atroviridis fruit extract in 3T3-L1 adipocyte cells. Journal of Biologically Active Products from Nature, 10 (4). pp. 303-316. ISSN 2231-1866 E-ISSN 2231-1874 https://www.tandfonline.com/doi/abs/10.1080/22311866.2020.1804448
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Darnis, Deny Susanti Hamidon, Hanisuhana Bakhtiar, M. Taher Ahmad Rusmili, Muhamad Rusdi Abd Hamid, Shafida Zakaria, Zainul Amiruddin Lipid accumulation modulation by garcinia atroviridis fruit extract in 3T3-L1 adipocyte cells
description	Garcinia atroviridis contains hydroxycitric acid (HCA) that affects obesity and obesityrelated diseases. This paper aims to quantify the amount of (HCA) available in the fruit extract of Garcinia atroviridis and investigate the lipid modulation properties possessed by the extract in the 3T3-L1 adipocyte cell line. High-performance liquid chromatography (HPLC) analysis was used to quantify HCA in the extract, whereas differentiated adipocytes were evaluated Oil Red O staining. The expression of PPARγ and C/EBPα was investigated using western blot, and the lipid content was confirmed using leptin ELISA assay. HPLC analysis quantified about 11.35 ± 0.55 % (w/w) of HCA in the fruit extract, with a retention time of 0.9 minutes corresponding to the standard. In the adipogenesis assay, an increased co-treatment concentration was observed and inhibited the formation of intracellular lipids. According to the western blot analysis, the treatment was found to significantly affect the expression of C/EBPα proteins solely. Meanwhile, the leptin assay finding was in agreement with the adipogenesis analysis as a decrement of leptin released was noted at increased treatment concentrations. Finally, the fruit extract enhanced the adipocyte breakdown (adipolysis) process as revealed by the glycerol concentration measured in the adipolysis assay. The findings indicated that the fruit extract of G. atroviridis played a role in the alteration of lipid accumulation processes by the intervention of proteins associated with adipogenesis and enhanced adipolysis.
format	Article
author	Darnis, Deny Susanti Hamidon, Hanisuhana Bakhtiar, M. Taher Ahmad Rusmili, Muhamad Rusdi Abd Hamid, Shafida Zakaria, Zainul Amiruddin
author_facet	Darnis, Deny Susanti Hamidon, Hanisuhana Bakhtiar, M. Taher Ahmad Rusmili, Muhamad Rusdi Abd Hamid, Shafida Zakaria, Zainul Amiruddin
author_sort	Darnis, Deny Susanti
title	Lipid accumulation modulation by garcinia atroviridis fruit extract in 3T3-L1 adipocyte cells
title_short	Lipid accumulation modulation by garcinia atroviridis fruit extract in 3T3-L1 adipocyte cells
title_full	Lipid accumulation modulation by garcinia atroviridis fruit extract in 3T3-L1 adipocyte cells
title_fullStr	Lipid accumulation modulation by garcinia atroviridis fruit extract in 3T3-L1 adipocyte cells
title_full_unstemmed	Lipid accumulation modulation by garcinia atroviridis fruit extract in 3T3-L1 adipocyte cells
title_sort	lipid accumulation modulation by garcinia atroviridis fruit extract in 3t3-l1 adipocyte cells
publisher	Taylor & Francis Online
publishDate	2020
url	http://irep.iium.edu.my/82840/1/Lipid%20Accumulation%20Modulation%20by%20Garcin...3-L1%20Adipocyte%20Cells%20_%20Enhanced%20Reader.pdf http://irep.iium.edu.my/82840/ https://www.tandfonline.com/doi/abs/10.1080/22311866.2020.1804448
_version_	1683230288428138496
score	13.211869

Lipid accumulation modulation by garcinia atroviridis fruit extract in 3T3-L1 adipocyte cells

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