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Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.

Researches concerning antioxidants are extensively studied nowadays. Antioxidants are well known to be associated with reduction in cancer incidence since they act against deteriorative effects of free radicals. These antioxidants include the yellow-to-red coloured pigments known as carotenoids, are...

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Main Authors: Mohd. Zaifuddin, Fatimah Azzah, Mohd Hassan, Norazian, Othman, Rashidi
Format: Conference or Workshop Item
Language:English
Published: 2011
Subjects:
QK Botany
SB Plant culture
Online Access:http://irep.iium.edu.my/8278/1/POSTER.pdf
http://irep.iium.edu.my/8278/
http://www.icnp2011.upm.edu.my
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spelling my.iium.irep.82782021-07-24T15:23:04Z http://irep.iium.edu.my/8278/ Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers. Mohd. Zaifuddin, Fatimah Azzah Mohd Hassan, Norazian Othman, Rashidi QK Botany SB Plant culture Researches concerning antioxidants are extensively studied nowadays. Antioxidants are well known to be associated with reduction in cancer incidence since they act against deteriorative effects of free radicals. These antioxidants include the yellow-to-red coloured pigments known as carotenoids, are essential for betterment in human immunity. Carotenoids can be found abundantly in dark green vegetables and also yellow, orange and red coloured fruits. They have to be taken up from daily diets since they cannot be produced by human biological system. Some carotenoids serve as pro-vitamin A that supple human visual and skin health. In this study, we aim to determine the carotenoid(s) content of Ruta angustifolia and their profile in the intact plant, callus and shoot cultures. The callus cultures were induced from stem explants and subsequently subcultured until the calli successfully generated in vitro shoots. The carotenoids extracts from the plant tissues were analysed qualitatively and quantitatively by using UV spectrophotometry and HPLC. The result showed that there was significant difference in accumulation of carotenoid(s) among the plant sources and the most common carotenoids found were β-carotene and lutein. The intact plant showed the highest content of the carotenoids whilst the callus possess the least. The outcome of this study could be used for further manipulation of the in vitro cultures for the carotenoids production enhancement. 2011-11-14 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/8278/1/POSTER.pdf Mohd. Zaifuddin, Fatimah Azzah and Mohd Hassan, Norazian and Othman, Rashidi (2011) Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers. In: International Conference on Natural Products (ICNP 2011): Metabolomics - A New Frontier in Natural Products Science, 14-16 November 2011, Putrajaya, Malaysia. http://www.icnp2011.upm.edu.my
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QK Botany
SB Plant culture
spellingShingle QK Botany
SB Plant culture
Mohd. Zaifuddin, Fatimah Azzah
Mohd Hassan, Norazian
Othman, Rashidi
Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
description Researches concerning antioxidants are extensively studied nowadays. Antioxidants are well known to be associated with reduction in cancer incidence since they act against deteriorative effects of free radicals. These antioxidants include the yellow-to-red coloured pigments known as carotenoids, are essential for betterment in human immunity. Carotenoids can be found abundantly in dark green vegetables and also yellow, orange and red coloured fruits. They have to be taken up from daily diets since they cannot be produced by human biological system. Some carotenoids serve as pro-vitamin A that supple human visual and skin health. In this study, we aim to determine the carotenoid(s) content of Ruta angustifolia and their profile in the intact plant, callus and shoot cultures. The callus cultures were induced from stem explants and subsequently subcultured until the calli successfully generated in vitro shoots. The carotenoids extracts from the plant tissues were analysed qualitatively and quantitatively by using UV spectrophotometry and HPLC. The result showed that there was significant difference in accumulation of carotenoid(s) among the plant sources and the most common carotenoids found were β-carotene and lutein. The intact plant showed the highest content of the carotenoids whilst the callus possess the least. The outcome of this study could be used for further manipulation of the in vitro cultures for the carotenoids production enhancement.
format Conference or Workshop Item
author Mohd. Zaifuddin, Fatimah Azzah
Mohd Hassan, Norazian
Othman, Rashidi
author_facet Mohd. Zaifuddin, Fatimah Azzah
Mohd Hassan, Norazian
Othman, Rashidi
author_sort Mohd. Zaifuddin, Fatimah Azzah
title Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
title_short Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
title_full Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
title_fullStr Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
title_full_unstemmed Determination of carotenoid(s) composition in intact plant, callus and shoot cultures of Ruta angustifolia (L.) Pers.
title_sort determination of carotenoid(s) composition in intact plant, callus and shoot cultures of ruta angustifolia (l.) pers.
publishDate 2011
url http://irep.iium.edu.my/8278/1/POSTER.pdf
http://irep.iium.edu.my/8278/
http://www.icnp2011.upm.edu.my
_version_ 1706956458930733056
score 13.211869

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