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Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis

A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL...

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Main Authors: Bakar, Nur Husnina Iffah, Lee, Thong Chuan, Mohd-Hairul, Ab Rahim, Ahmad Raus, Raha, Basri, Dayang Fredalina, Adzahar, Noor Suhana
Format: Book Chapter
Language:English
English
Published: Trans Tech Publications Ltd 2020
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/82587/1/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_scopus.pdf
http://irep.iium.edu.my/82587/7/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_ft.pdf
http://irep.iium.edu.my/82587/
https://www.scientific.net/MSF.981.265
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spelling my.iium.irep.825872020-09-02T07:55:50Z http://irep.iium.edu.my/82587/ Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis Bakar, Nur Husnina Iffah Lee, Thong Chuan Mohd-Hairul, Ab Rahim Ahmad Raus, Raha Basri, Dayang Fredalina Adzahar, Noor Suhana TP248.13 Biotechnology A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1- dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract. Trans Tech Publications Ltd 2020-03-27 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/82587/1/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_scopus.pdf application/pdf en http://irep.iium.edu.my/82587/7/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_ft.pdf Bakar, Nur Husnina Iffah and Lee, Thong Chuan and Mohd-Hairul, Ab Rahim and Ahmad Raus, Raha and Basri, Dayang Fredalina and Adzahar, Noor Suhana (2020) Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis. In: Materials Science Forum. Trans Tech Publications Ltd, Kuantan, Malaysia, pp. 265-270. ISBN 978-303571664-1 https://www.scientific.net/MSF.981.265 10.4028/www.scientific.net/MSF.981.265
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Bakar, Nur Husnina Iffah
Lee, Thong Chuan
Mohd-Hairul, Ab Rahim
Ahmad Raus, Raha
Basri, Dayang Fredalina
Adzahar, Noor Suhana
Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
description A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1- dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract.
format Book Chapter
author Bakar, Nur Husnina Iffah
Lee, Thong Chuan
Mohd-Hairul, Ab Rahim
Ahmad Raus, Raha
Basri, Dayang Fredalina
Adzahar, Noor Suhana
author_facet Bakar, Nur Husnina Iffah
Lee, Thong Chuan
Mohd-Hairul, Ab Rahim
Ahmad Raus, Raha
Basri, Dayang Fredalina
Adzahar, Noor Suhana
author_sort Bakar, Nur Husnina Iffah
title Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
title_short Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
title_full Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
title_fullStr Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
title_full_unstemmed Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
title_sort real-time cytotoxicity assay of water extracts hibiscus rosa-sinensis
publisher Trans Tech Publications Ltd
publishDate 2020
url http://irep.iium.edu.my/82587/1/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_scopus.pdf
http://irep.iium.edu.my/82587/7/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_ft.pdf
http://irep.iium.edu.my/82587/
https://www.scientific.net/MSF.981.265
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score 13.211869

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