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Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay

This study aimed to investigate the cytotoxicity of Lagerstroemia speciosa (L.) leaves crude extract. It has been reported to show various bioactivities. The phytochemical screening of the extract has been carried qualitatively. The cytotoxic effect was determined through in vitro MTT assay of M...

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Main Authors: Azad, Abul Kalam, Helal Uddin, A.B.M.
Format: Article
Language:English
English
Published: International Journal of Drug Delivery Technology 2020
Subjects:
QD Chemistry
RM Therapeutics. Pharmacology
RM300 Drugs and their action
RS Pharmacy and materia medica
RS192 Materia Medica-Pharmaceutical Technology
RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:http://irep.iium.edu.my/82575/7/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa%20%28L.%29%20leaves%20extract%20by%20MCF-7%20cell%20line%20and%20brine%20shrimp%20lethality%20bioassay.pdf
http://irep.iium.edu.my/82575/13/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa.pdf
http://irep.iium.edu.my/82575/
https://ijddt.com/volume10issue1/
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spelling my.iium.irep.825752022-11-07T02:24:41Z http://irep.iium.edu.my/82575/ Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay Azad, Abul Kalam Helal Uddin, A.B.M. QD Chemistry RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry This study aimed to investigate the cytotoxicity of Lagerstroemia speciosa (L.) leaves crude extract. It has been reported to show various bioactivities. The phytochemical screening of the extract has been carried qualitatively. The cytotoxic effect was determined through in vitro MTT assay of MCF-7 cell line, and brine shrimp lethality bioassay. The presence or absence of alkaloid, carbohydrate, glycoside, saponin, terpene, steroid, phenol, and flavonoid in the extract was determined through the qualitative tests. The extract showed cell viability of 100% (1.95–3.9 μL/mL), 96% (1.95 μL/mL), ≈ 95% (3.9–15.62 μL/ mL) and 88% (250 μL/mL) while the mortality of brine shrimp nauplii was from 5% to 10% (7.8 – 125 μL/mL) respectively. For both assays, DMSO of 1 & 0.1% were used as vehicle controls, while the potassium dichromate as the positive control for the brine shrimp only. These results proved the leaves extract to be non-toxic. International Journal of Drug Delivery Technology 2020-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82575/7/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa%20%28L.%29%20leaves%20extract%20by%20MCF-7%20cell%20line%20and%20brine%20shrimp%20lethality%20bioassay.pdf application/pdf en http://irep.iium.edu.my/82575/13/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa.pdf Azad, Abul Kalam and Helal Uddin, A.B.M. (2020) Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay. International Journal of Drug Delivery Technology, 10 (1). pp. 40-44. ISSN 0975-4415 https://ijddt.com/volume10issue1/ 10.25258/ijddt.10.1.6
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
RM Therapeutics. Pharmacology
RM300 Drugs and their action
RS Pharmacy and materia medica
RS192 Materia Medica-Pharmaceutical Technology
RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle QD Chemistry
RM Therapeutics. Pharmacology
RM300 Drugs and their action
RS Pharmacy and materia medica
RS192 Materia Medica-Pharmaceutical Technology
RS403 Materia Medica-Pharmaceutical Chemistry
Azad, Abul Kalam
Helal Uddin, A.B.M.
Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay
description This study aimed to investigate the cytotoxicity of Lagerstroemia speciosa (L.) leaves crude extract. It has been reported to show various bioactivities. The phytochemical screening of the extract has been carried qualitatively. The cytotoxic effect was determined through in vitro MTT assay of MCF-7 cell line, and brine shrimp lethality bioassay. The presence or absence of alkaloid, carbohydrate, glycoside, saponin, terpene, steroid, phenol, and flavonoid in the extract was determined through the qualitative tests. The extract showed cell viability of 100% (1.95–3.9 μL/mL), 96% (1.95 μL/mL), ≈ 95% (3.9–15.62 μL/ mL) and 88% (250 μL/mL) while the mortality of brine shrimp nauplii was from 5% to 10% (7.8 – 125 μL/mL) respectively. For both assays, DMSO of 1 & 0.1% were used as vehicle controls, while the potassium dichromate as the positive control for the brine shrimp only. These results proved the leaves extract to be non-toxic.
format Article
author Azad, Abul Kalam
Helal Uddin, A.B.M.
author_facet Azad, Abul Kalam
Helal Uddin, A.B.M.
author_sort Azad, Abul Kalam
title Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay
title_short Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay
title_full Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay
title_fullStr Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay
title_full_unstemmed Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay
title_sort phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (l.) leaves extract by mcf-7 cell line and brine shrimp lethality bioassay
publisher International Journal of Drug Delivery Technology
publishDate 2020
url http://irep.iium.edu.my/82575/7/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa%20%28L.%29%20leaves%20extract%20by%20MCF-7%20cell%20line%20and%20brine%20shrimp%20lethality%20bioassay.pdf
http://irep.iium.edu.my/82575/13/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa.pdf
http://irep.iium.edu.my/82575/
https://ijddt.com/volume10issue1/
_version_ 1751535892675166208
score 13.211869

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