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Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches

Lipid oxidation and microbial contamination are the major factors contributing to food deterioration. Food additives like antioxidants and antibacterials can prevent food spoilage by delaying oxidation and preventing the growth of bacteria. Artocarpus altilis leaves exhibited biological properties t...

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Main Authors: Ahmad, Mohammad Norazmi, Karim, Nazatul Umira, Normaya, Erna, Mat Piah, Bijarimi, Iqbal, Anwar, Ku Bulat, Ku Halim, ,
Format: Article
Language:English
English
Published: Nature Research 2020
Subjects:
Q Science (General)
TP Chemical technology
TP368 Food processing and manufacture
Online Access:http://irep.iium.edu.my/82234/1/82234_Artocarpus%20altilis%20extracts%20as%20a%20food-borne%20pathogen%20and%20oxidation%20inhibitors_ft.pdf
http://irep.iium.edu.my/82234/2/82234_Artocarpus%20altilis%20extracts%20as%20a%20food-borne%20pathogen%20and%20oxidation%20inhibitors_scopus.pdf
http://irep.iium.edu.my/82234/
https://www.nature.com/articles/s41598-020-66488-7
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spelling my.iium.irep.822342020-08-17T08:12:55Z http://irep.iium.edu.my/82234/ Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches Ahmad, Mohammad Norazmi Karim, Nazatul Umira Normaya, Erna Mat Piah, Bijarimi Iqbal, Anwar Ku Bulat, Ku Halim , Q Science (General) TP Chemical technology TP368 Food processing and manufacture Lipid oxidation and microbial contamination are the major factors contributing to food deterioration. Food additives like antioxidants and antibacterials can prevent food spoilage by delaying oxidation and preventing the growth of bacteria. Artocarpus altilis leaves exhibited biological properties that suggested its use as a new source of natural antioxidant and antimicrobial. Supercritical fluid extraction (SFE) was used to optimize the extraction of bioactive compounds from the leaves using response surface methodology (yield and antioxidant activity). The optimum SFE conditions were 50.5 °C temperature, 3784 psi pressure and 52 min extraction time. Verification test results (Tukey’s test) showed that no significant difference between the expected and experimental DPPH activity and yield value (99%) were found. Gas-chromatography –mass spectrometry (GC-MS) analysis revealed three major bioactive compounds existed in A. altilis extract. The extract demonstrated antioxidant and antibacterial properties with 2,3-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, ferric reducing ability of plasma (FRAP), hydroxyl radical scavenging activity, tyrosinase mushrrom inhibition of 41.5%, 8.15 ± 1.31 (µg of ascorbic acid equivalents), 32%, 37% and inhibition zone diameter of 0.766 ± 0.06 cm (B. cereus) and 1.27 ± 0.12 cm (E. coli). Conductor like screening model for real solvents (COSMO RS) was performed to explain the extraction mechanism of the major bioactive compounds during SFE. Molecular electrostatic potential (MEP) shows the probability site of nucleophilic and electrophilic attack during bacterial inhibition. Based on molecular docking study, non-covalent interactions are the main interaction occurring between the major bioactive compounds and bacteria (antibacterial inhibition). Nature Research 2020-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82234/1/82234_Artocarpus%20altilis%20extracts%20as%20a%20food-borne%20pathogen%20and%20oxidation%20inhibitors_ft.pdf application/pdf en http://irep.iium.edu.my/82234/2/82234_Artocarpus%20altilis%20extracts%20as%20a%20food-borne%20pathogen%20and%20oxidation%20inhibitors_scopus.pdf Ahmad, Mohammad Norazmi and Karim, Nazatul Umira and Normaya, Erna and Mat Piah, Bijarimi and Iqbal, Anwar and Ku Bulat, Ku Halim and UNSPECIFIED (2020) Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches. Scientific Reports, 10 (1). ISSN 2045-2322 https://www.nature.com/articles/s41598-020-66488-7 10.1038/s41598-020-66488-7
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic Q Science (General)
TP Chemical technology
TP368 Food processing and manufacture
spellingShingle Q Science (General)
TP Chemical technology
TP368 Food processing and manufacture
Ahmad, Mohammad Norazmi
Karim, Nazatul Umira
Normaya, Erna
Mat Piah, Bijarimi
Iqbal, Anwar
Ku Bulat, Ku Halim
,
Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches
description Lipid oxidation and microbial contamination are the major factors contributing to food deterioration. Food additives like antioxidants and antibacterials can prevent food spoilage by delaying oxidation and preventing the growth of bacteria. Artocarpus altilis leaves exhibited biological properties that suggested its use as a new source of natural antioxidant and antimicrobial. Supercritical fluid extraction (SFE) was used to optimize the extraction of bioactive compounds from the leaves using response surface methodology (yield and antioxidant activity). The optimum SFE conditions were 50.5 °C temperature, 3784 psi pressure and 52 min extraction time. Verification test results (Tukey’s test) showed that no significant difference between the expected and experimental DPPH activity and yield value (99%) were found. Gas-chromatography –mass spectrometry (GC-MS) analysis revealed three major bioactive compounds existed in A. altilis extract. The extract demonstrated antioxidant and antibacterial properties with 2,3-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, ferric reducing ability of plasma (FRAP), hydroxyl radical scavenging activity, tyrosinase mushrrom inhibition of 41.5%, 8.15 ± 1.31 (µg of ascorbic acid equivalents), 32%, 37% and inhibition zone diameter of 0.766 ± 0.06 cm (B. cereus) and 1.27 ± 0.12 cm (E. coli). Conductor like screening model for real solvents (COSMO RS) was performed to explain the extraction mechanism of the major bioactive compounds during SFE. Molecular electrostatic potential (MEP) shows the probability site of nucleophilic and electrophilic attack during bacterial inhibition. Based on molecular docking study, non-covalent interactions are the main interaction occurring between the major bioactive compounds and bacteria (antibacterial inhibition).
format Article
author Ahmad, Mohammad Norazmi
Karim, Nazatul Umira
Normaya, Erna
Mat Piah, Bijarimi
Iqbal, Anwar
Ku Bulat, Ku Halim
,
author_facet Ahmad, Mohammad Norazmi
Karim, Nazatul Umira
Normaya, Erna
Mat Piah, Bijarimi
Iqbal, Anwar
Ku Bulat, Ku Halim
,
author_sort Ahmad, Mohammad Norazmi
title Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches
title_short Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches
title_full Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches
title_fullStr Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches
title_full_unstemmed Artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: RSM, COSMO RS, and molecular docking approaches
title_sort artocarpus altilis extracts as a food-borne pathogen and oxidation inhibitors: rsm, cosmo rs, and molecular docking approaches
publisher Nature Research
publishDate 2020
url http://irep.iium.edu.my/82234/1/82234_Artocarpus%20altilis%20extracts%20as%20a%20food-borne%20pathogen%20and%20oxidation%20inhibitors_ft.pdf
http://irep.iium.edu.my/82234/2/82234_Artocarpus%20altilis%20extracts%20as%20a%20food-borne%20pathogen%20and%20oxidation%20inhibitors_scopus.pdf
http://irep.iium.edu.my/82234/
https://www.nature.com/articles/s41598-020-66488-7
_version_ 1675327185516232704
score 13.211869

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