Staff View: Detection of pork in processed meat products by species-specific PCR for halal verification: food fraud cases in Hat Yai, Thailand

Detection of pork in processed meat products by species-specific PCR for halal verification: food fraud cases in Hat Yai, Thailand

Consumer confidence in halal integrity of the unique and various food products provides Hat Yai, Thailand a great potential for a global destination of Muslim-friendly tourism. Islam prohibits the consumption of pork and its derivatives in any food products. The issue of food adulteration and contam...

Full description

Saved in:

Bibliographic Details
Main Authors:	Mahamad Maifiah, Mohd Hafidz, Makatar, W. H., Adilan, H., Nawawee, T.
Format:	Article
Language:	English English
Published:	Rynnye Lyan Resources 2020
Subjects:	HD9000 Special industries and trades QP Physiology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/81585/1/81585_Detection%20of%20pork%20in%20processed%20meat.pdf http://irep.iium.edu.my/81585/2/81585_Detection%20of%20pork%20in%20processed%20meat_SCOPUS.pdf http://irep.iium.edu.my/81585/ https://www.myfoodresearch.com/uploads/8/4/8/5/84855864/_31__fr-2019-s21_mohd_hafidz.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.iium.irep.81585
record_format	dspace
spelling	my.iium.irep.815852020-11-12T05:42:56Z http://irep.iium.edu.my/81585/ Detection of pork in processed meat products by species-specific PCR for halal verification: food fraud cases in Hat Yai, Thailand Mahamad Maifiah, Mohd Hafidz Makatar, W. H. Adilan, H. Nawawee, T. HD9000 Special industries and trades QP Physiology TP248.13 Biotechnology Consumer confidence in halal integrity of the unique and various food products provides Hat Yai, Thailand a great potential for a global destination of Muslim-friendly tourism. Islam prohibits the consumption of pork and its derivatives in any food products. The issue of food adulteration and contamination, particularly in the processed halal meat products with pork and its derivatives, greatly concern Muslim consumers. The aim of this study was to detect the presence of pork DNA from processed meat products collected from self-proclaimed “halal” Muslim street food stalls at Hat Yai, Thailand. Thirty-six samples of various processed meat products were randomly collected from seven Muslim street food stalls including patties, meatballs, and sausages containing processed chicken, beef, or a mixture of various meats. The detection of the presence of pork and its derivatives was performed by a conventional polymerase chain reaction (PCR) technique based on the pork-specific primers for a conserved region in the mitochondrial (mt) 12S ribosomal RNA (rRNA) gene. The results revealed that three out of the thirty-six samples (8.3%) were positively identified to contain porcine DNA by the detection of the expected single band of size 387 bp. The DNA method conveniently provides reliable results for routine food analysis for halal requirement. Overall, the study highlights the importance of halal integrity between the producers, suppliers, and street food business owners to provide halal food products particularly to Muslim consumers. © 2020 The Authors. Rynnye Lyan Resources 2020-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/81585/1/81585_Detection%20of%20pork%20in%20processed%20meat.pdf application/pdf en http://irep.iium.edu.my/81585/2/81585_Detection%20of%20pork%20in%20processed%20meat_SCOPUS.pdf Mahamad Maifiah, Mohd Hafidz and Makatar, W. H. and Adilan, H. and Nawawee, T. (2020) Detection of pork in processed meat products by species-specific PCR for halal verification: food fraud cases in Hat Yai, Thailand. Food Research, 4 ((S1)). pp. 244-249. E-ISSN 2550-2166 https://www.myfoodresearch.com/uploads/8/4/8/5/84855864/_31__fr-2019-s21_mohd_hafidz.pdf 10.26656/fr.2017.4(S1).S21
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English
topic	HD9000 Special industries and trades QP Physiology TP248.13 Biotechnology
spellingShingle	HD9000 Special industries and trades QP Physiology TP248.13 Biotechnology Mahamad Maifiah, Mohd Hafidz Makatar, W. H. Adilan, H. Nawawee, T. Detection of pork in processed meat products by species-specific PCR for halal verification: food fraud cases in Hat Yai, Thailand
description	Consumer confidence in halal integrity of the unique and various food products provides Hat Yai, Thailand a great potential for a global destination of Muslim-friendly tourism. Islam prohibits the consumption of pork and its derivatives in any food products. The issue of food adulteration and contamination, particularly in the processed halal meat products with pork and its derivatives, greatly concern Muslim consumers. The aim of this study was to detect the presence of pork DNA from processed meat products collected from self-proclaimed “halal” Muslim street food stalls at Hat Yai, Thailand. Thirty-six samples of various processed meat products were randomly collected from seven Muslim street food stalls including patties, meatballs, and sausages containing processed chicken, beef, or a mixture of various meats. The detection of the presence of pork and its derivatives was performed by a conventional polymerase chain reaction (PCR) technique based on the pork-specific primers for a conserved region in the mitochondrial (mt) 12S ribosomal RNA (rRNA) gene. The results revealed that three out of the thirty-six samples (8.3%) were positively identified to contain porcine DNA by the detection of the expected single band of size 387 bp. The DNA method conveniently provides reliable results for routine food analysis for halal requirement. Overall, the study highlights the importance of halal integrity between the producers, suppliers, and street food business owners to provide halal food products particularly to Muslim consumers. © 2020 The Authors.
format	Article
author	Mahamad Maifiah, Mohd Hafidz Makatar, W. H. Adilan, H. Nawawee, T.
author_facet	Mahamad Maifiah, Mohd Hafidz Makatar, W. H. Adilan, H. Nawawee, T.
author_sort	Mahamad Maifiah, Mohd Hafidz
title	Detection of pork in processed meat products by species-specific PCR for halal verification: food fraud cases in Hat Yai, Thailand
title_short	Detection of pork in processed meat products by species-specific PCR for halal verification: food fraud cases in Hat Yai, Thailand
title_full	Detection of pork in processed meat products by species-specific PCR for halal verification: food fraud cases in Hat Yai, Thailand
title_fullStr	Detection of pork in processed meat products by species-specific PCR for halal verification: food fraud cases in Hat Yai, Thailand
title_full_unstemmed	Detection of pork in processed meat products by species-specific PCR for halal verification: food fraud cases in Hat Yai, Thailand
title_sort	detection of pork in processed meat products by species-specific pcr for halal verification: food fraud cases in hat yai, thailand
publisher	Rynnye Lyan Resources
publishDate	2020
url	http://irep.iium.edu.my/81585/1/81585_Detection%20of%20pork%20in%20processed%20meat.pdf http://irep.iium.edu.my/81585/2/81585_Detection%20of%20pork%20in%20processed%20meat_SCOPUS.pdf http://irep.iium.edu.my/81585/ https://www.myfoodresearch.com/uploads/8/4/8/5/84855864/_31__fr-2019-s21_mohd_hafidz.pdf
_version_	1683230349684899840
score	13.211869

Detection of pork in processed meat products by species-specific PCR for halal verification: food fraud cases in Hat Yai, Thailand

Similar Items