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The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney

Introduction: Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Exposure to arsenic has been linked with multiple health problems. Inflammatory genes such as interleukin 6 (IL-6) and interleukin 8 (IL-8) play an important role in pathophysiology of exposu...

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Main Authors: Wan Salleh, Wan Muhamad Salahudin, A.Talib, Norlelawati, Abdullah, Nor Zamzila, Buyong, Zunariah
Format: Conference or Workshop Item
Language:English
Published: 2020
Subjects:
RA1190 Toxicology
Online Access:http://irep.iium.edu.my/77425/1/77425_The%20effect%20of%20low%20dose%20organic%20arsenic.pdf
http://irep.iium.edu.my/77425/
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spelling my.iium.irep.774252020-01-30T01:32:49Z http://irep.iium.edu.my/77425/ The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney Wan Salleh, Wan Muhamad Salahudin A.Talib, Norlelawati Abdullah, Nor Zamzila Buyong, Zunariah RA1190 Toxicology Introduction: Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Exposure to arsenic has been linked with multiple health problems. Inflammatory genes such as interleukin 6 (IL-6) and interleukin 8 (IL-8) play an important role in pathophysiology of exposure to an acute high dose arsenic-mediated nephrotoxicity which led to proximal tubular injury. However, studies focusing on low dose organic arsenic exposure and its adverse effects on kidneys are limited. Objective: To study the effects of low dose arsenic exposure on the inflammatory genes expression in rats’ kidneys at three different duration intervals; 2 months, 4 months and 6 months. Materials and Method: Thirty-six male Sprague-Dawley rats were randomly divided into six groups (n=6); a treatment group and its control for each interval. The treatment groups were given daily oral gavage of MSMA at 63.0 mg/kg body weight (BW) which is equivalent to 1/20 LD50 of MSMA. While control groups received distilled water via oral gavage. At the end of study intervals, the kidney tissues were harvested for arsenic level analysis and molecular analysis. The RNA integrity was confirmed with Qiaxcel analysis. The expressions of inflammatory genes were analysed using RT2 SYBR Green qPCR Mastermix. Results: Tissue arsenic concentration were higher in all treated group. Both IL-6 and IL-8 showed a similar pattern of expressions. Organic arsenic down-regulated IL-6 and IL-8 in 2-month (both fold change -1.03) and 6-month groups (fold change -1.36,-1.15). However in the 4-month group, both IL-6 and IL-8 were up-regulated (both fold change 1.31). Conclusion: Interestingly, these findings suggest that low dose arsenic exposure has shown anti-inflammatory effect at 2-month and 6-month, however 4-month paradoxically demonstrated pro-inflammatory effect which consistent with the tissue arsenic levels. 2020 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/77425/1/77425_The%20effect%20of%20low%20dose%20organic%20arsenic.pdf Wan Salleh, Wan Muhamad Salahudin and A.Talib, Norlelawati and Abdullah, Nor Zamzila and Buyong, Zunariah (2020) The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney. In: Kuantan Research Day 2019, 23rd October 2019, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/index.php/events/show/kuantan-research-day-2019
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RA1190 Toxicology
spellingShingle RA1190 Toxicology
Wan Salleh, Wan Muhamad Salahudin
A.Talib, Norlelawati
Abdullah, Nor Zamzila
Buyong, Zunariah
The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
description Introduction: Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Exposure to arsenic has been linked with multiple health problems. Inflammatory genes such as interleukin 6 (IL-6) and interleukin 8 (IL-8) play an important role in pathophysiology of exposure to an acute high dose arsenic-mediated nephrotoxicity which led to proximal tubular injury. However, studies focusing on low dose organic arsenic exposure and its adverse effects on kidneys are limited. Objective: To study the effects of low dose arsenic exposure on the inflammatory genes expression in rats’ kidneys at three different duration intervals; 2 months, 4 months and 6 months. Materials and Method: Thirty-six male Sprague-Dawley rats were randomly divided into six groups (n=6); a treatment group and its control for each interval. The treatment groups were given daily oral gavage of MSMA at 63.0 mg/kg body weight (BW) which is equivalent to 1/20 LD50 of MSMA. While control groups received distilled water via oral gavage. At the end of study intervals, the kidney tissues were harvested for arsenic level analysis and molecular analysis. The RNA integrity was confirmed with Qiaxcel analysis. The expressions of inflammatory genes were analysed using RT2 SYBR Green qPCR Mastermix. Results: Tissue arsenic concentration were higher in all treated group. Both IL-6 and IL-8 showed a similar pattern of expressions. Organic arsenic down-regulated IL-6 and IL-8 in 2-month (both fold change -1.03) and 6-month groups (fold change -1.36,-1.15). However in the 4-month group, both IL-6 and IL-8 were up-regulated (both fold change 1.31). Conclusion: Interestingly, these findings suggest that low dose arsenic exposure has shown anti-inflammatory effect at 2-month and 6-month, however 4-month paradoxically demonstrated pro-inflammatory effect which consistent with the tissue arsenic levels.
format Conference or Workshop Item
author Wan Salleh, Wan Muhamad Salahudin
A.Talib, Norlelawati
Abdullah, Nor Zamzila
Buyong, Zunariah
author_facet Wan Salleh, Wan Muhamad Salahudin
A.Talib, Norlelawati
Abdullah, Nor Zamzila
Buyong, Zunariah
author_sort Wan Salleh, Wan Muhamad Salahudin
title The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
title_short The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
title_full The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
title_fullStr The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
title_full_unstemmed The effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
title_sort effect of low dose organic arsenic exposure on inflammatory genes expression in rat’s kidney
publishDate 2020
url http://irep.iium.edu.my/77425/1/77425_The%20effect%20of%20low%20dose%20organic%20arsenic.pdf
http://irep.iium.edu.my/77425/
http://www.iium.edu.my/index.php/events/show/kuantan-research-day-2019
_version_ 1657565960240889856
score 13.211869

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