Cover Image

Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology

Abstract: Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, wh...

Full description

Saved in:
Bibliographic Details
Main Authors: Mahmud, Israa Ali, Mirghani, Mohamed Elwathig Saeed, Yusof, Faridah, Al-Khatib, Ma'an Fahmi Rashid
Format: Article
Language:English
Published: MDPI 2019
Subjects:
T Technology (General)
Online Access:http://irep.iium.edu.my/75015/1/preprints201907.0055.v1.pdf
http://irep.iium.edu.my/75015/
https://www.preprints.org/manuscript/201907.0055/v1
https://www.preprints.org/manuscript/201907.0055/v1
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.iium.irep.75015
record_format dspace
spelling my.iium.irep.750152024-05-02T07:12:10Z http://irep.iium.edu.my/75015/ Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Yusof, Faridah Al-Khatib, Ma'an Fahmi Rashid T Technology (General) Abstract: Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, while NEPP is neglected. Thus, this study aimed to release NEPP from the remaining powder residue of Barhi date palm kernels (BDPK) with acid hydrolysis. The related extraction conditions were determined and optimised using response surface methodology (RSM) for maximisation of NEPP with highest cytotoxic and antioxidant activities. The face-centred central composite design (FCCCD) was used to establish treatments based on three independent variables, namely; extraction temperature, time, and solvent/sample ratio. Under the optimal conditions, the experimental values for DPPH radical-scavenging capacity of NEPP (IC50=57.52µg/mL), and cytotoxicity of NEPP against A549 and HT29 cells were IC50=17.4 µg/mL and 31.4µg/mL, respectively. The experimental values were in agreement with those predicted by RSM models, confirming the suitability of the model employed and the success of RSM for optimisation of the extraction conditions for NEPP from BDPK. These results indicate that NEPP from industrial date fruit waste could be a promising candidate as natural antioxidants with significant antiproliferation effect against A549 and HT29 cancer cells in-vitro. MDPI 2019-07 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/75015/1/preprints201907.0055.v1.pdf Mahmud, Israa Ali and Mirghani, Mohamed Elwathig Saeed and Yusof, Faridah and Al-Khatib, Ma'an Fahmi Rashid (2019) Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology. Preprints. pp. 1-36. https://www.preprints.org/manuscript/201907.0055/v1 https://www.preprints.org/manuscript/201907.0055/v1
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Mahmud, Israa Ali
Mirghani, Mohamed Elwathig Saeed
Yusof, Faridah
Al-Khatib, Ma'an Fahmi Rashid
Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
description Abstract: Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, while NEPP is neglected. Thus, this study aimed to release NEPP from the remaining powder residue of Barhi date palm kernels (BDPK) with acid hydrolysis. The related extraction conditions were determined and optimised using response surface methodology (RSM) for maximisation of NEPP with highest cytotoxic and antioxidant activities. The face-centred central composite design (FCCCD) was used to establish treatments based on three independent variables, namely; extraction temperature, time, and solvent/sample ratio. Under the optimal conditions, the experimental values for DPPH radical-scavenging capacity of NEPP (IC50=57.52µg/mL), and cytotoxicity of NEPP against A549 and HT29 cells were IC50=17.4 µg/mL and 31.4µg/mL, respectively. The experimental values were in agreement with those predicted by RSM models, confirming the suitability of the model employed and the success of RSM for optimisation of the extraction conditions for NEPP from BDPK. These results indicate that NEPP from industrial date fruit waste could be a promising candidate as natural antioxidants with significant antiproliferation effect against A549 and HT29 cancer cells in-vitro.
format Article
author Mahmud, Israa Ali
Mirghani, Mohamed Elwathig Saeed
Yusof, Faridah
Al-Khatib, Ma'an Fahmi Rashid
author_facet Mahmud, Israa Ali
Mirghani, Mohamed Elwathig Saeed
Yusof, Faridah
Al-Khatib, Ma'an Fahmi Rashid
author_sort Mahmud, Israa Ali
title Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
title_short Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
title_full Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
title_fullStr Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
title_full_unstemmed Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
title_sort effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
publisher MDPI
publishDate 2019
url http://irep.iium.edu.my/75015/1/preprints201907.0055.v1.pdf
http://irep.iium.edu.my/75015/
https://www.preprints.org/manuscript/201907.0055/v1
https://www.preprints.org/manuscript/201907.0055/v1
_version_ 1800081747118915584
score 13.211869

Similar Items