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Engineered meniscus scaffolds using sonication decellularization treatment system

Meniscus located in the weight-bearing area responsible for the movement and functions of the knee. However, the frequent injuries within the avascular region of meniscus have lack of healing capability. Thus, the emerging decellularized scaffolds serve as one the interventions for the regeneration...

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Main Authors: Yusof, Fatihah, Sha'ban, Munirah, Noor Azmi, Azran Azhim
Format: Article
Language:English
English
English
Published: Penerbit UTHM 2019
Subjects:
TA164 Bioengineering
Online Access:http://irep.iium.edu.my/74941/6/74941_Engineered%20meniscus%20scaffolds%20using%20sonication%20decellularization%20treatment%20system_Scopus.pdf
http://irep.iium.edu.my/74941/11/74941_Engineered%20Meniscus%20Scaffolds%20using%20Sonication%20_wos.pdf
http://irep.iium.edu.my/74941/17/74941_Engineered%20meniscus%20scaffolds%20using%20sonication%20decellularization%20treatment%20system.pdf
http://irep.iium.edu.my/74941/
https://publisher.uthm.edu.my/ojs/index.php/ijie/article/view/4542/2944
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spelling my.iium.irep.749412020-06-29T01:14:22Z http://irep.iium.edu.my/74941/ Engineered meniscus scaffolds using sonication decellularization treatment system Yusof, Fatihah Sha'ban, Munirah Noor Azmi, Azran Azhim TA164 Bioengineering Meniscus located in the weight-bearing area responsible for the movement and functions of the knee. However, the frequent injuries within the avascular region of meniscus have lack of healing capability. Thus, the emerging decellularized scaffolds serve as one the interventions for the regeneration of new tissues to treat early degenerative joint disease. The aim of this study is to investigate the effectiveness of sonication treatment system in decellularization of meniscus tissues. The decellularization process was conducted in 40 kHz frequency with 0.1% SDS solution for 10 hours and proceeds with five days washing process. The decellularization efficiency was evaluated through histology, gel electrophoresis and biochemical assays to observe the cellular components removal and preservation of extracellular matrix (ECM). Compared to the control group, the histological evaluation of sonication decellularized scaffolds based on staining van Gieson showed complete removal of cellular components. Picrosirius red and Safranin O/fast green staining revealed the well preservation of the distribution of collagen and glycosaminoglycans (GAGs) networks in sonication decellularized scaffolds and no visible of DNA bands in the electrophoresis of agarose gel. Biochemical assessment for DNA quantification illustrated a significant decrement of DNA residues and GAGs for sonication decellularized scaffolds while maintained in collagen content. Based on the results, it can be deduced that sonication decellularization treatment system successfully prepared scaffolds with low cellular contents and maintained extracellular matrix components. Therefore, sonication decellularization treatment system can serve as one of the potential physical decellularization method in tissue engineering and regenerative medicine fields. Penerbit UTHM 2019-09-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/74941/6/74941_Engineered%20meniscus%20scaffolds%20using%20sonication%20decellularization%20treatment%20system_Scopus.pdf application/pdf en http://irep.iium.edu.my/74941/11/74941_Engineered%20Meniscus%20Scaffolds%20using%20Sonication%20_wos.pdf application/pdf en http://irep.iium.edu.my/74941/17/74941_Engineered%20meniscus%20scaffolds%20using%20sonication%20decellularization%20treatment%20system.pdf Yusof, Fatihah and Sha'ban, Munirah and Noor Azmi, Azran Azhim (2019) Engineered meniscus scaffolds using sonication decellularization treatment system. International Journal of Integrated Engineering, 11 (3 Special Issue 2019: Electrical and Electronic Engineering). pp. 81-89. ISSN 2229-838X E-ISSN 2600-7916 https://publisher.uthm.edu.my/ojs/index.php/ijie/article/view/4542/2944 10.30880/ijie.2019.11.03.009
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic TA164 Bioengineering
spellingShingle TA164 Bioengineering
Yusof, Fatihah
Sha'ban, Munirah
Noor Azmi, Azran Azhim
Engineered meniscus scaffolds using sonication decellularization treatment system
description Meniscus located in the weight-bearing area responsible for the movement and functions of the knee. However, the frequent injuries within the avascular region of meniscus have lack of healing capability. Thus, the emerging decellularized scaffolds serve as one the interventions for the regeneration of new tissues to treat early degenerative joint disease. The aim of this study is to investigate the effectiveness of sonication treatment system in decellularization of meniscus tissues. The decellularization process was conducted in 40 kHz frequency with 0.1% SDS solution for 10 hours and proceeds with five days washing process. The decellularization efficiency was evaluated through histology, gel electrophoresis and biochemical assays to observe the cellular components removal and preservation of extracellular matrix (ECM). Compared to the control group, the histological evaluation of sonication decellularized scaffolds based on staining van Gieson showed complete removal of cellular components. Picrosirius red and Safranin O/fast green staining revealed the well preservation of the distribution of collagen and glycosaminoglycans (GAGs) networks in sonication decellularized scaffolds and no visible of DNA bands in the electrophoresis of agarose gel. Biochemical assessment for DNA quantification illustrated a significant decrement of DNA residues and GAGs for sonication decellularized scaffolds while maintained in collagen content. Based on the results, it can be deduced that sonication decellularization treatment system successfully prepared scaffolds with low cellular contents and maintained extracellular matrix components. Therefore, sonication decellularization treatment system can serve as one of the potential physical decellularization method in tissue engineering and regenerative medicine fields.
format Article
author Yusof, Fatihah
Sha'ban, Munirah
Noor Azmi, Azran Azhim
author_facet Yusof, Fatihah
Sha'ban, Munirah
Noor Azmi, Azran Azhim
author_sort Yusof, Fatihah
title Engineered meniscus scaffolds using sonication decellularization treatment system
title_short Engineered meniscus scaffolds using sonication decellularization treatment system
title_full Engineered meniscus scaffolds using sonication decellularization treatment system
title_fullStr Engineered meniscus scaffolds using sonication decellularization treatment system
title_full_unstemmed Engineered meniscus scaffolds using sonication decellularization treatment system
title_sort engineered meniscus scaffolds using sonication decellularization treatment system
publisher Penerbit UTHM
publishDate 2019
url http://irep.iium.edu.my/74941/6/74941_Engineered%20meniscus%20scaffolds%20using%20sonication%20decellularization%20treatment%20system_Scopus.pdf
http://irep.iium.edu.my/74941/11/74941_Engineered%20Meniscus%20Scaffolds%20using%20Sonication%20_wos.pdf
http://irep.iium.edu.my/74941/17/74941_Engineered%20meniscus%20scaffolds%20using%20sonication%20decellularization%20treatment%20system.pdf
http://irep.iium.edu.my/74941/
https://publisher.uthm.edu.my/ojs/index.php/ijie/article/view/4542/2944
_version_ 1672610134125182976
score 13.211869

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